array_files=new Array(); array_files[0]=new Array(0,1,"http://www.imgge.bg.ac.yu/Srb/index.htm","2009-10-24","16K","IMGGI :: Pocetna ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGI :: Pocetna Institut za Molekularnu Genetiku i Genetičko Inženjerstvo (IMGGI – nekadašnji Centar za genetičko inženjerstvo) osnovan je 1986. godine, pošto je Jugoslovenski parlament usvojio Zakon o ratifikaciji statuta Internacionalnog Centra za Genetičko Inženjerstvo i Biotehnologiju (ICGEB).Na ovaj način Institut je povezan sa ICGEB-om u Trstu, Italija, i kao njegov pridruženi centar i uključen je u sve aktivnosti ove međunarodne organizacije.Većina aktivnosti Instituta usmerena je ka fundamentalnim istraživanjima u molekularnoj biologiji, molekularnoj genetici, tehnologiji rekombinantne DNK i biotehnologiji. U oblasti fundamentalnih istraživanja, projekti Instituta su najvećim delom fokusirani na istraživanja vezana za analizu genomske organizacije i regulacije genske ekspresije kod raznih organizama. Ruski naučnici u poseti IMGGI Janez Potočnik, evropski komesar za nauku i Božidar Đelić, ministar za nauku i tehnološki razvoj RS  posetili IMGGI Prezentacija firme LKB Alfatrade: Prezentacija i demonstracija rada QIAcube aparata Arhiva vesti ... Pogledajte naše slike ... IMGGE (bivši Centar za Gen. inženjerstvo) je osnovan 1986 godine ... Do nas možete doći autobuskim linijama : 33, 39 ili 25 ... Ministarstvo Nauke Univerzitet Beograd ICGEB ostali ... "); array_files[1]=new Array(0,1,"http://www.imgge.bg.ac.yu/Srb/seminari.htm","2009-10-24","15K","IMGGI :: Seminari i predavanja ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGI :: Seminari i predavanja Pocetna Datum Predavač Tema 29.09.2009 Lab03 Izvestaji sa kongresa 02.07.2009 dr Mariano Barbacid Cyclin-Dependent kinases, Cell Cycle and cancer 16.06.2009 dr Djordje Francuski Crystal Structure of the Antitoxin-Toxin Protein Complex RelB-RelEfrom Methanococcus jannaschii 09.06.2009 dr Karmen Stankov Genetika multifaktorijelnih oboljenja: uloga stresa endoplazmatskog retikuluma u predispoziciji za nastanak dijabetes melitus-a tip 1 05/06.2009 Lab03 Journal club 04.2009 Lab05 Seminar 02/03.2009 Lab03 Journal club 03.02.2009 Lab01 Izvestaji sa kongresa Upravni odbor, Direktor, Zamenik direktora, Naučno veće, Laboratorije ... Predsednik Upravnog odbora, Sekretar za finansije ... Do nas možete doći autobuskim linijama : 33, 39 ili 25 ... Pogledajte naše slike ... Ministarstvo Nauke Beogradski Univerzitet ICGEB ostali ... "); array_files[2]=new Array(0,1,"http://www.imgge.bg.ac.yu/Srb/Vesti/alphatrade/alphatrade.htm","2009-10-24","14K","IMGGE : Alphatrade ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGE : Alphatrade Pocetna U Institutu za molekularnu genetiku i geneticko inženjerstvo, 07.05.2009., održana je prezentacija i demonstracija rada QIAcube aparata za automatsku izolaciju DNK i RNK iz razlicitih uzoraka, kao i QIAxcel aparata za automatsku analizu fragmenata DNK i RNK proizvodjaca QIAGEN, u organizaciji firme ALFATRADE ENTERPRIZE D. O. O iz Beograda. Teorijsku i prakticnu prezentaciju održala je dr. sci. Carola Schade, zamenik direktora za automatizaciju firme Qiagen, a u prezentaciji su ucestvovali i predstavnici firme Mediline iz Slovenije. Demonstraciji su prisustvovale kolege iz Instituta za biološka istraživanja Siniša Stankovic, Instuta za zaštitu bilja i životnu sredinu, Instituta za kukuruz i laboratorijeA BIO TECH LAB D.O.O. Upravni odbor, Direktor, Zamenik direktora, Naučno veće, Laboratorije ... Predsednik Upravnog odbora, Sekretar za finansije ... Do nas možete doći autobuskim linijama : 33, 39 ili 25 ... Pogledajte naše slike ... Ministarstvo Nauke Beogradski Univerzitet ICGEB ostali ... "); array_files[3]=new Array(0,1,"http://www.imgge.bg.ac.yu/Srb/Vesti/janez/potocnik.htm","2009-10-24","14K","IMGGE : Poseta IMGGI ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGE : Poseta IMGGI Pocetna 29. juna 2009.godine Janez Potocnik, evropski komesar za nauku i Božidar Delic, ministar za nauku i tehnološki razvoj RS su posetili IMGGI, u pratnji: Žozep M. Ljoveras, Ambasador, šef Delegacije Evropske komisije u Republici Srbiji, Dankarlo Karati, Šef Odeljenja A, Generalna Direkcija za istraživanje i razvoj, Tanja Fridrih, Generalna Direkcija za istraživanje i tehnološki razvoj, zadužena za medunarodnu dimenziju Framework programa, Kristina Bole, clanica kabineta Janeza Potocnika Aleksandar Dordevic, Delegacija Evropske komisije u Republici Srbiji Upravni odbor, Direktor, Zamenik direktora, Naučno veće, Laboratorije ... Predsednik Upravnog odbora, Sekretar za finansije ... Do nas možete doći autobuskim linijama : 33, 39 ili 25 ... Pogledajte naše slike ... Ministarstvo Nauke Beogradski Univerzitet ICGEB ostali ... "); array_files[4]=new Array(0,1,"http://www.imgge.bg.ac.yu/Srb/Vesti/rusi.htm","2009-10-24","14K","IMGGE : Poseta ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGE : Poseta Pocetna U organizaciji Ministarstva obrazovanja i nauke Ruske Federacije, Državne Agencije za nauku i inovacije „Inovatika“ i „Ruskog doma“ u Beogradu je od 28. septembra do 1. oktobra 2009. godine održan stručni seminar-izložba «Ruske biotehnologije». Cilj  ovog projekta je prikazivanje rezultata iz perspektivnih oblasti naučnih istraživanja i dostignuća, uspostavljanje naučnih i poslovnih veza sa vodećim naučno-istraživačkim institucijama i proizvodnim organizacijama u Srbiji čija je delatnost vezana za biotehnologije. Tokom trajanja seminara grupa ruskih naučnika, u sastavu: FEOFANOV S.A. – zamenik direktora Filijale Instituta za bio-organsku hemiju „Šemjakin“ (Puščinski naučni centar), LEONTIJEVSKI A.A. – zamenik direktora Instituta za biohemiju i fiziologiju mikroorganizma „G.K.Skrjabin“ Ruske Akademije Nauka (Puščinski naučni centar), JAROCKI S.V. – zamenik direktora Državnog naučno- istraživačkog instituta (NII) genetike i selekcije industrijskih organizma, IŠČENKO A.M. – upravnik laboratorije za biohemiju belančevina Državnog NII idealno čistih preparata (SPb) i AVČIJEVA P.B. – upravnik laboratorije biološki aktivnih materija Državnog NII „Sintezbelok“ je posetila IMGGI i razmatrala mogućnost uspostavljanje institucionalnih veza između naučnika u perspektivnim oblastima biotehnologija. Upravni odbor, Direktor, Zamenik direktora, Naučno veće, Laboratorije ... Predsednik Upravnog odbora, Sekretar za finansije ... Do nas možete doći autobuskim linijama : 33, 39 ili 25 ... Pogledajte naše slike ... Ministarstvo Nauke Beogradski Univerzitet ICGEB ostali ... "); array_files[5]=new Array(0,1,"http://www.imgge.bg.ac.yu/index.htm","2009-10-24","16K","IMGGE : About ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGE : About Institute of Molecular Genetics and Genetic Engineering (IMGGE - formerly Centre for Genetic Engineering) was founded in 1986. The Institute is associated to International Center for Genetic Engineering and Biotechnology (ICGEB), Trieste, Italy, as an affiliated centre and it is involved in all activities within this international organization. In the field of fundamental research, Institutes projects are mostly focused on research related to analysis of genome organisation and regulation of gene expression in different organisms. Furthermore, IMGGE is also oriented to biotechnological and commercial applications in human and veterinary medicine, agriculture, food production, and pharmaceutical industry. Janez Potočnik, European Commissioner for Science and Research and Božidar Djelić, Minister of Science and Technological Development of the Republic of Serbia visited IMGGE Foundation “Goran Ljubijankić” Representatives of the World Bank visited IMGGI Science Festival, 5th - 7th December 2008 ICGEB funding programmes See our history, events or take virtual tour photos ... IMGGE (formerly Center for Genetic Engineering) was founded in 1986 ... You can reach us by bus: 33, 39 or 25 ... Ministry of Science Belgrade University ICGEB more links ... "); array_files[6]=new Array(0,1,"http://www.imgge.bg.ac.yu/","2009-10-24","16K","IMGGE : About ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGE : About Institute of Molecular Genetics and Genetic Engineering (IMGGE - formerly Centre for Genetic Engineering) was founded in 1986. The Institute is associated to International Center for Genetic Engineering and Biotechnology (ICGEB), Trieste, Italy, as an affiliated centre and it is involved in all activities within this international organization. In the field of fundamental research, Institutes projects are mostly focused on research related to analysis of genome organisation and regulation of gene expression in different organisms. Furthermore, IMGGE is also oriented to biotechnological and commercial applications in human and veterinary medicine, agriculture, food production, and pharmaceutical industry. Janez Potočnik, European Commissioner for Science and Research and Božidar Djelić, Minister of Science and Technological Development of the Republic of Serbia visited IMGGE Foundation “Goran Ljubijankić” Representatives of the World Bank visited IMGGI Science Festival, 5th - 7th December 2008 ICGEB funding programmes See our history, events or take virtual tour photos ... IMGGE (formerly Center for Genetic Engineering) was founded in 1986 ... You can reach us by bus: 33, 39 or 25 ... Ministry of Science Belgrade University ICGEB more links ... "); array_files[7]=new Array(0,4,"http://www.imgge.bg.ac.yu/Srb/Files/april%202009%20Laboratorija%20za%20molekularnu%20biologiji%20aktinomiceta%20-%20seminar.pdf","2009-10-24","48K"," Seminar_05.xls ","","","SEMINAR Laboratorija za molekularnu genetiku aktinomiceta 7. april 2009 14. april 2009 21. april 2009 28. april 2009 Branka Vasiljevi Ivana Mori Tatjana Ili-Tomi Miloje Savi Tanja Naranci Lidija Djoki Nada Stankovi Sanja Bajki Opsti pregled istrazivackog interesa Laboratorije za molekularnu genetiku aktinomiceta 16S rRNK metiltransferaze kod patogena Regulacija ekspresije sgm gena Struktura i funkcija Sgm proteina Mikrobioloski diverzitet u ekoloski interesantnim stanistima Fenol degradujuce bakterije Karekterizacija klastera za sintezu bioaktivnog jedinjenja iz Streptomyces durmitorensis Ekspresija GrmA metiltransferaze iz Micromonospora purpurea "); array_files[8]=new Array(0,4,"http://www.imgge.bg.ac.yu/Srb/Files/09%20jun%202009%20predavanje%20dr%20Karmen%20Stankov.pdf","2009-10-24","39K"," Predavanje_gost.xls ","","","Predavanje --------------------------------------------- 09.06.09 Dr Karmen Stankov - Katedra za biohemiju, Medicinski fakultet, Novi Sad Genetika multifaktorijelnih oboljenja: uloga stresa endoplazmatskog retikuluma u predispoziciji za nastanak dijabetes melitus-a tip 1 Dokument izradio: Miroslav Konstantinovi jun 2009. "); array_files[9]=new Array(0,4,"http://www.imgge.bg.ac.yu/Srb/Files/16%20jun%202009%20-%20predavanje%20dr%20Dorde%20Francuski.pdf","2009-10-24","41K"," Predavanje_gost.xls ","","","Predavanje - Biblioteka IMGGI 15 h --------------------------------------------- 16.06.09 Dr Djordje Francuski Freie Universitat in Berlin Crystal Structure of the Antitoxin-Toxin Protein Complex RelB-RelE from Methanococcus jannaschii Dokument izradio: Miroslav Konstantinovi jun 2009. "); array_files[10]=new Array(0,4,"http://www.imgge.bg.ac.yu/Srb/Files/03%20februar%202009%20-%20izvestaji%20sa%20kongresa.pdf","2009-10-24","55K"," Izvestaji.xls ","","","IZVESTAJI SA KONGRESA I DRUGIH BORAVAKA VAN INSTITUTA LAB 01 Branka Zuki, 03.02.09 Maja Stojiljkovi Natasa Tosi th 40 European Human Genetics Conference, Barselona, Maj 2008. 13th European Hematology Association Congress, Kopenhagen, jun 2008. Society for the Study of Inborn Errors of Metabolism Annual Symposium, Lisabon, Septembar 2008 2nd Educational Workshop on Ig Gene Analysis in Chronic Lymphocytic Leukemia, Pariz Life Learning Centar Training, Bolonja, Oktobar 2008. () Human Genome Organisation Mutation Detection Training Course, Roterdam, Novembar 2008. Maja Stojiljkovi Teodora Karan Branka Zuki, Milena Radmilovi Maja Stojiljkovi "); array_files[11]=new Array(0,4,"http://www.imgge.bg.ac.yu/Srb/Files/februar-mart%202009%20Laboratorija%20za%20molekularnu%20biologiju%20-%20journal%20club.pdf","2009-10-24","13K"," JC_03.xls ","","","Journal Club Laboratorija za molekularnu biologiju 24. 2. 2009. Kojic Snezana 3. 3. 2009. Rakicevic Ljiljana Ljuic Mila Marco M. Candeias et al , (2008). p53 mRNA controls p53 activity by managing Mdm2 Functions, Nature Cell Biology, 10, 1098-1105 Chen LL et al. (2008). Alu element-mediated gene silencing. EMBO J. 27,1694-1705. Nurit GM et al . (2008). Alternative splicing of Alu exons--two arms are better than one. Nucleic Acids Research, 36, 2012 10. 3. 2009. Marija Stankovic Swinburne I et al. (2008). Intron length increases oscillatory periods of gene expression in animal cells. Genes and Development, 22, 2342-2346 Cai L et al . (2008). Frequency-modulated nuclear localization bursts coordinate gene regulation. Jelena Kusic Nature. 455, 485-90. 17. 3. 2009. Dragica Radojkovic Rogers CS et al. (2008). Disruption of the CFTR gene produces a model of cystic fibrosis in newborn pigs. Science, 321, 1837-41 Dufour E et al . (2008). Age-associated mosaic respiratory chain deficiency causes trans-neuronal Aleksandra Divac degeneration. Human Molecular Genetics, 17, 1418­1426 Tomic Branko Cadoret J et al. (2008). Genome-wide studies highlight indirect links between human replication origins and gene regulation. Proc Natl Acad Sci U S A., 105, 15837-42. 24. 3. 2009. Huppert JL et al . (2008). G-quadruplexes: the beginning and end of UTRs. Nucleic Acids Research, Valentina Djordjevic 36, 6260­6268 Aleksandra Nestorovic Steele AD et al . (2008). Heat shock factor 1 regulates lifespan as distinct from disease onset in prion disease. PNAS, 105, 13626­13631 31. 3. 2009. Andersen FF et al . (2008). Assembly and structural analysis of a covalently closed nano-scale DNA Aleksandra Nikolic cage. Nucleic Acids Research, 36, 1113­1119 "); array_files[12]=new Array(0,4,"http://www.imgge.bg.ac.yu/Srb/Files/maj-jun%202009%20Laboratorija%20za%20molekularnu%20biologiju%20biljaka%20-%20journal%20club.pdf","2009-10-24","97K"," JC_08.xls ","","","Journal Club Laboratorija za molekularnu biologiju biljaka 05.05. 2009. 12.05.2009. 19.05.2009 RNA-directed DNA methylation induces transcriptional activation in plants-PNAS, February 3, 2009 Defensin-like polypeptide LUREs are pollen tube attractants secreted from synergid cells ­ Nature Svetlana Radovi (2009) 458, 357-361 (19 March 2009). Sorting of Small RNAs into Arabidopsis Argonaute Complexes Is Directed by the 5 Terminal Vesna Maksimovi Nucleotide, Shijun Mi et al. 2008, Cell 133, 116-127 Gordana Timotijevi Arabidopsis ACCELERATED CELL DEATH2 Modulates Programmed Cell Death - Nan Yao and Jean T. Greenberg - The Plant Cell, 2006.,18, 397-411 Zivko Jovanovi Bojana Banovi A putative ABC transporter confers durable resistance to multiple fungal pathogens in wheat Krattinger, SG et al. Science. 2009 Mar 6;323(5919):1301-2. Rice XB15, a Protein Phosphatase 2C,Negatively Regulates Cell Death and XA21-Mediated Innate Immunity Chang-Jin Park et al. PloS Biology, September 2008. XopD SUMO protease affects host transcription, promotes pathogen growth and delayes symptom development in Xanthomonas - infected tomato leaves Jung-Gun Kim et al., The Plant Cell , July 2008. Enrichment of tomato fruit with health-promoting anthocyanins by expression by select transcription factors ­ Nature Biotechnology (2008) 26, 1301-1308, 26 october 2008. 26.05.2009 Jelena Samardzi Dragana Maji 02.06.2009 Jovanka Miljus Djuki Miroslav Konstantinovi Isolating mRNA using acupuncture What do you get when you cross eastern medicine with molecular biology? "); array_files[13]=new Array(0,4,"http://www.imgge.bg.ac.yu/Srb/Files/02%20jul%202009%20-%20predavanje%20dr%20Mariano%20Barbacid.pdf","2009-10-24","35K"," Microsoft Word - 02 jul 2009 - predavanje dr Mariano Barbacid.doc ","","","U cetvrtak, 2. jula 2009. godine, u Institutu za molekularnu genetiku i geneticko inzenjerstvo, Vojvode Stepe 444a, Beograd 10 h Odbrana doktorske disertacije Mr Jelena Urosevi Centro Nacional de Investigaciones Oncologicas, Madrid PROUCAVANJE ULOGE B-RAF PROTEINA U KANCERU KORISENJEM ZIVOTINJSKIH TUMOR MODELA 12 h Predavanje Dr Mariano Barbacid Centro Nacional de Investigaciones Oncologicas, Madrid CYCLIN-DEPENDENT KINASES, CELL CYCLE AND CANCER Dr Mariano Barbacid je veliko ime u oblasti istrazivanja kancera. U njegovoj laboratoriji je izolovan prvi ljudski onkogen. Svetska zdravstvena organizacija mu je 2007. godine dodelila Medal of Honour of the International Agency for Research on Cancer. "); array_files[14]=new Array(0,4,"http://www.imgge.bg.ac.yu/Srb/Files/septembar-oktobar%202009%20-%20izvestaji%20sa%20kongresa.pdf","2009-10-24","127K"," Izvestaji_sept_okt_2009.xls ","","","IZVESTAJI SA KONGRESA I DRUGIH BORAVAKA VAN INSTITUTA Biblioteka IMGGI 15 casova 29.09.09 Aleksandar Krsti Branko Tomi Jelena Kusi Marija Stankovi Valentina Djordjevi International Summer School Stem cells and regenerative medicine, Piran 2009 EMBO Practical Course Single-molecule manipulation and analysis of DNA-protein interactions, 5. do 18. jula 2009. godine u Parizu. Chromatin, Replication and Chromosomal Stability (Kopenhagen, 15-16 jun 2009.) Molecular and cellular aspects of chronic lung disease, 18-20 June, 2009 Rotterdam. XXIIst Congress of the International Society on Thrombosis and Haemostasis, 11. do 16. jula 2009. godine u Bostonu. Dokument izradio: Miroslav Konstantinovi septembar 2009. "); array_files[15]=new Array(0,1,"http://www.imgge.bg.ac.yu/Srb/Laboratorije/08/comservices.htm","2009-10-24","15K","IMGGI :: Komercijalne usluge ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGI :: Komercijalne usluge Pocetna > Laboratorije > Laboratorija za Molekularnu Biologiju Biljaka GENETIČKI MODIFIKOVANE BILJKE U Laboratoriji za molekularnu biologiju biljaka razvijena je i standardizovana usluga Detekcija, identifikacija i kvantifikacija genetičkih modifikacija prisutnih kod biljaka i hrane biljnog porekla, primenom metode kvalitativnog PCR i kvantitativnog RealTime PCR. Na osnovu potreba tržišta i zahteva EU, a definisano republičkim Zakonom o GMO, IMGGI je ovlašćena institucija za vršenje ove usluge od strane MInistartva za poljoprivredu i vodoprivredu (od 2001. god), a od 2002.godine uključen u medjunaridni sistem kontrole kvaliteta (ISTA proficiency test) Metoda obuhvata izolovanje DNK iz dostavljenog materijala i odgovarajuće PCR analize: Kvalitativna a) Nespecifikovana GMO analiza (utvrdjivanje prisustva CaMV35S promotora i NOS terminatora) b) Utvrdjivanje definisane genetičke modifikacije: tolerancija na herbicide: soja RoundUp Ready, kukuruz NK 603, GA 21 i Liberty Link -T25, uljana repica Ms8, RT 73, Rf3, šećerna repa H7-1, pirinač LLrice 62 rezistencija na insekte : kukuruz Bt176, Bt11 i MON810 Granica osetljivosti metode je 1 pg DNK, što omogućuje detektovanje: u uzorcima semena sa samo 0.01% GMO (jedno GM zrno u 10 000) · proizvodima za humanu ishranu i hranu za životinje: lecitin, brašno, proteinski izolati, smeše, konditorski proizvodi Kvantitativna Real Time PCR test na aparatu Applied Biosystems, ABI Prism 7500 METODA JE POUZDANA, OSETLJIVA I STANDARDIZOVANA PREMA KRITERIJUMIMA VAŽEĆIM U ODGOVARAJUĆIM LABORATORIJAMA EU: PROCES AKREDITACIJE JE U TOKU Kontakt: Dr Vesna Maksimović, Institut za molekularnu genetiku i genetičko inženjerstvo, V. Stepe 444a, P. Fah 23, 11010 Beograd, SRBIJA TEL/FAX: ++ 381 11 3976 414 e-mail: vesamax@imgge.bg.ac.rs Završeni i aktivni projekti, istraživački uspesi ... Naši projekti objavljeni u arhivama i časopisima bioloških nauka ... U našoj laboratoriji radi 4 Dr, 5 Mr i jedan BSc ... Ministarstvo Nauke Beogradski Univerzitet ICGEB ostali "); array_files[16]=new Array(0,1,"http://www.imgge.bg.ac.yu/Srb/Laboratorije/06/comservices.htm","2009-10-24","21K","IMGGI :: Komercijalne usluge ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGI :: Komercijalne usluge Pocetna > Laboratorije > Lab. za M. Genetiku Industrijskih Mikroorganizama Komercijalna ponuda: Kompletna tehnologija za proizvodnju probiotičkog mlečnog napitka, bazirana na autohtonim starter  kulturama. Kompletna tehnologija za proizvodnju jogurta, bazirana na autohtonim starter kulturama. Kompletna tehnologija za proizvodnju polutvrdog sira tipa trapista i belog sira, bazirana na autohtonim starter kulturama. Molekularna dijagnostika patogenih mikroorganizama u uzorcima različitog porekla. A. Starter kulture za proizvodnju fermentisanih mlečnih proizvoda Na osnovu dugogodišnje selekcije bakterija mlečne kiseline (BMK) poreklom iz različitih fermentisanih mlečnih proizvoda pravljenih u domaćinstvima širom bivše Jugoslavije izdvojene su odredjene vrste BMK koje su detaljno biohemijski i genetički okarakterisane u Institutu za molekularnu genetiku i genetičko inženjerstvo, Beograd. Na osnovu tih istraživanja konstruisane su različite starter kulture sa tačno definisanim karakteristikama za proizvodnju fernentisanih napitaka i sireva. Konstruisane starter kulture su testirane u industrijskim uslovima u mlekara u Srbiji 1. ACIDOVIT Proizvodnja ACIDOVITA fermentisanog dijetalnog kiselog mlečnog napitka od obranog pasterizovanog mleka. Proizvodnja Acidovita je pokušaj da se u uslovima standardne proizvodnje jogurta, a bez većih prethodnih tehničko-tehnoloških zahvata u cilju stvaranja optimalnih uslova (potrebno je ubaciti u proizvodnu liniju samo duplikator sa hladjenjem), proizvede dijetetski napitak sa 9,5% suve materije (0.1% mlečna mast, 0,5% mineralne materije, 3,9% proteina i 5,0% laktoze). Energetska vrednost ACIDOVITA iznosi 151,58 kJ/100 gr ili 312,57 kJ u pakovanju od 0,2 l. Pored nutritivnih svojstava koje poseduju svi kiselo mlečni fermentisani napici, ACIDOVIT pokazuje i odredjena terapeutska svojstva zahvaljujući prisustvu intestinalne vrste bakterija Lactobacillus helveticus, koja pokazuje izrazito antimikrobno delovanje, jer zaustavlja rast štetnih mikroorganiza "); array_files[17]=new Array(0,1,"http://www.imgge.bg.ac.yu/Srb/Laboratorije/05/comservices.htm","2009-10-24","13K","IMGGI :: Komercijalne usluge ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGI :: Komercijalne usluge Pocetna > Laboratorije > Laboratorija za Molekularnu Genetiku Aktinomiceta R.br. Komercijalne usluge Cena (rsd) 1 Expression of human interferon ß 2 Overexpression of Providencia rettgeri Penicillin Amidase Gene Završeni i aktivni projekti, istraživački uspesi ... Naši projekti objavljeni u arhivama i časopisima bioloških nauka ... U našoj laboratoriji radi 4 Dr, 5 Mr i jedan BSc ... Ministarstvo Nauke Beogradski Univerzitet ICGEB ostali ... "); array_files[18]=new Array(0,1,"http://www.imgge.bg.ac.yu/Srb/Laboratorije/04/comservices.htm","2009-10-24","14K","IMGGI :: Komercijalne usluge ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGI :: Komercijalne usluge Pocetna > Laboratorije > Laboratorija za Humanu Molekularnu Genetiku R.br. Komercijalne usluge Cena (rsd) 1 Primena Fluorescentne in situ Hibridizacije (FISH) u detekciji numeričkih i strukturnih aberacija hromozoma 2 Molekularna dijagnostika poremećaja u razviću pola kod ljudi 3 Molekularna dijagnostika BLAD-a (Bovine Leukocyte Adhesion Deficiency) kod goveda 4 RFLP analiza genskih varijanti proteina mleka 5 Molekularna dijagnostika stresnog sindroma svinja Završeni i aktivni projekti, istraživački uspesi ... Naši projekti objavljeni u arhivama i časopisima bioloških nauka ... U našoj laboratoriji radi 4 Dr, 5 Mr i jedan BSc ... Ministarstvo Nauke Beogradski Univerzitet ICGEB ostali ... "); array_files[19]=new Array(0,1,"http://www.imgge.bg.ac.yu/Srb/Laboratorije/03/comservices.htm","2009-10-24","14K","IMGGI :: Komercijalne usluge ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGI :: Komercijalne usluge Pocetna > Laboratorije > Laboratorija za Molekularnu Biologiju R.br. Komercijalna usluga Cena (rsd) 1 Molekularna dijagnostika cistične fibroze 2 Molekularna dijagnostika deficijencije alfa 1 antitripsina 3 Detekcija Leiden mutacije 4 Detekcija of FII G20210A mutacije 5 Detekcija of MTHFR C667T mutacije Završeni i aktivni projekti, istraživački uspesi ... Naši projekti objavljeni u arhivama i časopisima bioloških nauka ... U našoj laboratoriji radi 4 Dr, 5 Mr i jedan BSc ... Ministarstvo Nauke Beogradski Univerzitet ICGEB ostali ... 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Naziv usluge Cena (rsd) 1 Dizajniranje prajmera 4.720,00 2 Detekcija mutacija u genu za TPMT (izbor terapijskih protokola za ALL, AML, kancer, auto-imune bolesti) 10.620,00 3 Molekularna dijagnostika alpha-talasemija 14.160,00 4 Molekularna dijagnostika beta-talasemija 14.160,00 5 Prenatalna dijagnostika talasemijskih sindroma 5.900,00 6 Detekcija minimalne rezidualne bolesti kod ALL (t(4;11), t(9;22), t(12; 21), t( 1;19)) 14.160,00 7 Detekcija minimalne rezidualne bolesti kod AML i CML (t(15, 17), t(8; 21), inv16, t(9; 22)) 14.160,00 8 Molekularna dijagnostika najčešćih translokacija kod ALL (t(4; 11), t(9; 22), t(12; 21), t(1; 19)) 16.520,00 9 Detekcija mutacija u Flt 3 genu (prognoza AML) 5.900,00 10 Karakterizacija rearanžmana u genima za Ig i TCR 10.620,00 11 PCR analiza sa proverom 3.540,00 12 Izolovanje DNK sa proverom 3.540,00 13 Izolacija RNK sa proverom 3.540,00 14 Detekcija mutacije V617F u genu za JAK2 Završeni i aktivni projekti, istraživački uspesi ... Naši projekti objavljeni u arhivama i časopisima bioloških nauka ... U našoj laboratoriji radi 4 Dr, 5 Mr i jedan BSc ... Ministarstvo Nauke Beogradski Univerzitet ICGEB ostali ... "); array_files[21]=new Array(0,1,"http://www.imgge.bg.ac.yu/Srb/comercialservices.htm","2009-10-24","28K","IMGGI :: Komercijalne usluge ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGI :: Komercijalne usluge Pocetna R.broj Naziv usluge Cena (rsd) 1 FISH na preparatima periferne krvi (numeričke aberacije hromozoma 1,13/21,18, X i Y) 9.440,00 2 FISH na histološkim preparatima 12.980,00 3 Obeležavanje proba za flourescentnu hibridizaciju (10 pločica) 8.260,00 4 Detekcija delecije 22q11 primenom FISH-a 10.620,00 5 Kvalitativni PCR - Detekcija CaMV 35S promotora NOS terminatora »Round Up Ready« konstrukta u materijalu koji sadrži DNK soje 10.620,00 6 Kvalitativni PCR - Detekcija CaMV 35S promotora »Round Up Ready« konstrukta i genskih konstrukata karakterističnih za LibertyLink-T25, Bt11 i MON810 u materijalu koji sadrži DNK kukuruza 14.160,00 7 Izdavanje GMO nalaza na engleskom jeziku 3.000,00 8 Kvantifikacija prisustva genetički modifikovanog materijala 16.284,00 9 Analiza varijanti gena za kapa-kazein goveda 3.894,00 10 Analiza varijanti gena za beta-laktoglobulin goveda 3.894,00 11 Detekcija SRY gena na uzorku periferne krvi 4.720,00 12 Detekcija SRY gena na uzorku plodove vode 7.080,00 13 Detekcija SRY gena na uzorku gonadalnog tkiva  7.080,00 14 Kvantifikacija prisustva genetički modifikovanog materijala 16.284,00 15 Molekularna dijagnostika cistične fibroze (7 najčešćih mutacija) 14.750,00 16 Prenatalna dijagnostika cistične fibroze 11.800,00 17 Molekularna dijagnostika S i Z mutacija A1AT gena 9.086,00 18 Pretraživanje celog CFTR gena 1 (egzoni: 1,3,4,5,6a,7,9,10, 11,12,13,14a,15,17b,19,20) 59.000,00 19 Pretraživanje celog CFTR gena2(egzoni 2,6b,8,14b,16,17a,18,21, 22,23,24) 35.400,00 20 Molekularna dijagnostika mutacije FV Leiden 6.490,00 21 Molekularna dijagnostika mutacije protrombin FII G20210A 6.490,00 22 Molekularna dijagnostika mutacije MTHFR C677T 6.490,00 23 Molekularna dijagnostika mutacije FV i FII 9.086,00 24 Molekularna dijagnostika mutacije FV i MTHFR 9.086,00 25 Molekularna dijagnostika mutacije FII i MTHFR 9.086,00 26 Molekularna dijagnostika mutacije FV, FII i MTHFR 15.576,00 27 Molekularna dijagnostika CFTR F508del mutacije 7.080,00 28 Detekcija mutacija u genu za "); array_files[22]=new Array(0,1,"http://www.imgge.bg.ac.yu/Srb/education.htm","2009-10-24","17K","IMGGI :: Edukacija ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGI :: Edukacija Pocetna Teorijski i praktični kursevi za studente dodiplomskih i poslediplomskih studija Teorijski i praktični kursevi: “Osnovne metode genetičkog inženjerstva” “Elektroforeza proteina: teorija i praksa” “Detekcija genetičkih modifikacija u biljnom materijalu i hrani” Molekularna  dijagnostika bolesti čoveka” (1,63 Mb) Mogućnost izrade eksperimentalnog dela diplomskih radova i doktorskih teza Individualni praktični kursevi- obuka u ovladavanju tehnikama molekularne genetike i genetičkog inženjerstva Kontakt: beogene@eunet.rs Naziv studijskog programa Doktorske studije Molekularne biologije Naziv ustanova u kojima se organizuje zajednički studijski program 1. Institut za molekularnu genetiku i genetičko inženjerstvo, Univerzitet u Beogradu (IMGGI), 2. Institut za biološka istraživanja „Siniša Stanković“, Univerzitet u Beogradu (IBISS), 3. Biološki fakultet, Univerzitet u Beogradu (BF). Visokoškolska ustanova u kojoj se izvodi studijski program Biološki fakultet, Univerzitet u Beogradu Obrazovno-naučno polje Prirodno-matematičke nauke Naučna oblast Biološke nauke Obim studija izražen u ESPB bodovima 180 Naziv diplome Doktor nauka-biološke nauke-molekularna biologija Dužina studija tri (3) godine Godina u kojoj je započela realizacija studijskog programa školska 2006-2007. Planirani broj studenata koji se upisuje godišnje 20 Jezik na kome se izvodi studijski program srpski, engleski Godina kada je program akreditovan 2008. Web adresa na kojoj se nalaze informacije o studijskom programu www.bio.bg.ac.rs Upravni odbor, Direktor, Zamenik direktora, Naučno veće, Laboratorije ... Predsednik Upravnog odbora, Sekretar za finansije ... Do nas možete doći autobuskim linijama : 33, 39 ili 25 ... Pogledajte naše slike ... Ministarstvo Nauke Beogradski Univerzitet ICGEB ostali ... "); array_files[23]=new Array(0,1,"http://www.imgge.bg.ac.yu/Laboratorije/08/comservices.htm","2009-10-24","12K","IMGGE :: Commercial services ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGE :: Commercial services Home > Laboratories > Laboratory for Plant Molecular Biology No Commercial service Price (rsd) 1 Geneticaly modified organisms Finished and ongoing projects, specific research goals ... List of Publications : Team members : Ministry of Science Belgrade University ICGEB more links ... "); array_files[24]=new Array(0,1,"http://www.imgge.bg.ac.yu/Laboratorije/06/comservices.htm","2009-10-24","12K","IMGGE :: Commercial services ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGE :: Commercial services Home > Laboratories > Lab. for Molecular Genetics of Industrial Microorganisms No Commercial service Price (rsd) 1 Starter Cultures for Production of Fermented Milk Products 2 Detection of Pathogenic Microorganisms from Clinical Specimens Team members : Finished and ongoing projects, specific research goals ... List of Publications : Ministry of Science Belgrade University ICGEB more links ... "); array_files[25]=new Array(0,1,"http://www.imgge.bg.ac.yu/Laboratorije/05/comservices.htm","2009-10-24","13K","IMGGE :: Commercial services ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGE :: Commercial services Home > Laboratories > Laboratory for Molecular Genetics of Actinomycetes No Commercial service Price (rsd) 1 Expression of human interferon ß 2 Overexpression of Providencia rettgeri Penicillin Amidase Gene Finished and ongoing projects, specific research goals ... List of Publications : Team members : Ministry of Science Belgrade University ICGEB more links ... "); array_files[26]=new Array(0,1,"http://www.imgge.bg.ac.yu/Laboratorije/04/comservices.htm","2009-10-24","14K","IMGGE :: Commercial services ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGE :: Commercial services Home > Laboratories > Laboratory for Human Molecular Genetics No Commercial service Price (rsd) 1 Application of Fluorescent in situ Hybridization (FISH) for detection of numerical and structural chromosome aberrations 2 Detection of SRY gene in patients with sexual development disorders 3 Molecular diagnostics of BLAD (Bovine Leukocyte Adhesion Deficiency) 4 PCR-RFLP genotyping of milk protein variants 5 Molecular diagnostics of porcine stress syndrome Finished and ongoing projects, specific research goals ... List of Publications : Team members : Ministry of Science Belgrade University ICGEB more links ... "); array_files[27]=new Array(0,1,"http://www.imgge.bg.ac.yu/Laboratorije/03/comservices.htm","2009-10-24","13K","IMGGE :: Commercial services ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGE :: Commercial services Home > Laboratories > Laboratory for Molecular Biology No Commercial service Price (rsd) 1 Molecular diagnostics of cystic fibrosis 2 Molecular diagnostics of alpha-1-antytripsin deficiency 3 Detection of FV Leiden mutation 4 Detection of FII G20210A mutation 5 Detection of MTHFR C667T mutation Finished and ongoing projects, specific research goals ... List of Publications : Team members : Ministry of Science Belgrade University ICGEB more links ... "); array_files[28]=new Array(0,1,"http://www.imgge.bg.ac.yu/Laboratorije/01/comservices.htm","2009-10-24","16K","IMGGE :: Commercial services ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGE :: Commercial services Home > Laboratories > Laboratory for Molecular Hematology No Commercial service Price (rsd) 1 Molecular diagnostics of pheniketonuria 4.720,00 2 Detection of TPMT (thyopurine-S-methyltransferase) gene mutations 10.620,00 3 Molecular diagnostics of alpha-thalassemia 14.160,00 4 Molecular diagnostics of beta-thalassemia 14.160,00 5 Molecular diagnostics of thalassemia syndromes 5.900,00 6 Detection of minimal residual disease in ALL 14.160,00 7 Detection of minimal residual disease in AML and CML 14.160,00 8 Molecular diagnostics of translocations in ALL 16.520,00 9 Detection of Flt3 gene mutations 5.900,00 10 Characterization of rearangements in Ig ang TCR genes 10.620,00 11 PCR analysis (without primers) 3.540,00 12 DNA extraction 3.540,00 13 RNA extraction 3.540,00 14 Detection of the JAK2 mutation Finished and ongoing projects, specific research goals ... List of Publications : Team members : Ministry of Science Belgrade University ICGEB more links ... "); array_files[29]=new Array(0,1,"http://www.imgge.bg.ac.yu/education.htm","2009-10-24","17K","IMGGE :: Education ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGE :: Education Home Theoretical and practical courses at the undergraduate and postgraduate level Practical and theoretical courses: “Basic methods in genetic engineering” “Protein electrophoresis: theory and practice” “Detection of genetic modifications in plant material and food” Molecular Diagnostics of Human Diseases” (1,63 Mb) Experimental and theoretical requirements for BSc, MSc and PhD degrees Individual practical courses - Training in mastering experimental techniques of the molecular genetics and genetic engineering Contact : beogene@eunet.rs PhD programme Molecular Biology Institutions engaged in the realization of the PhD programme 1. Institute of Molecular Genetics and Genetic Engineering, University of Belgrade 2 Institute for Biological Research “Siniša Stanković”, University of Belgrade 3. University of Belgrade, Faculty of Biology Higher education unit engaged in the organization of the PhD programme University of Belgrade, Faculty of Biology Educational-scientific field Natural Science Scientific field Biology ECTS points 180 Title PhD in Biology-Molecular Biology Duration of the PhD programme Three (3) years Starting year of the PhD programme 2006-2007. Number of students per a year 20 Languages used in the PhD programme Serbian, English Accreditation 2008. Web address www.bio.bg.ac.rs Organisational Chart Director, Chairman of the Board ... You can reach us by bus: 33, 39 or 25 ... See our history, events or take virtual tour photos ... Ministry of Science Belgrade University ICGEB more links ... "); array_files[30]=new Array(0,1,"http://www.imgge.bg.ac.yu/comercialservices.htm","2009-10-24","20K","IMGGE :: Commercial services ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGE :: Commercial services Home No Service name 1 Application of Fluorescent in situ hybridization (FISH) for detection of numerical and structural chromosome aberrations 2 Detection of SRY gene in patients with sexual development disorders 3 Genetically modified organisms 4 Molecular diagnostics of cystic fibrosis 5 Molecular diagnostics of alpha-1-antytripsin deficiency 6 Detection of FV Leiden mutation 7 Detection of FII G20210A mutation 8 Detection of MTHFR C667T mutation 9 Detection of TPMT (thyopurine-S-methyltransferase) gene mutations 10 Molecular diagnostics of BLAD (Bovine Leukocyte Adhesion Deficiency) 11 Molecular diagnostics of porcine stress syndrome 12 Molecular diagnostics of alpha-thalassemia 13 Molecular diagnostics of beta-thalassemia 14 Molecular diagnostics of thalassemia syndromes 15 Detection of minimal residual disease in AML and CML 16 Detection of minimal residual disease in ALL 17 PCR-RFLP genotyping of milk protein variants 18 Molecular diagnostics of translocations in ALL 19 Detection of Flt3 gene mutations 20 Characterization of rearrangements in Ig and TCR genes 21 PCR analysis (without primers) 22 Primer design 23 DNA extraction 24 RNA extraction 25 Detection of the JAK2 mutation 26 Analysis of products obtained by genetic modification of microorganisms 27 Cultivation of primary fibroblast culture and preparation for autolog transplantation 28 Total protein isolation 29 SDS-PAGE 30 Molecular diagnostics of microorganisms 31 Referent strains 32 Fingerprint analysis of microorganisms 33 Determination of microorganisms by sequencing of 16S rDNK 34 Starter cultures for production of fermented milk products 35 Detection of pathogenic microorganisms from clinical specimens 36 Expression of human interferon ß 37 Overexpression of Providencia rettgeri Penicillin Amidase Gene Organisational Chart Director, Chairman of the Board ... You can reach us by bus: 33, 39 or 25 ... See our history, events or take virtual tour photos ... Ministry of Science Belgrade University ICGEB more links . "); array_files[31]=new Array(0,1,"http://www.imgge.bg.ac.yu/Srb/Services/cf.htm","2009-10-14","14K","IMGGI :: Cisticna fibroza ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGI :: Cisticna fibroza Pocetna > Komercijalne usluge DGGE analiza CFTR exon 12 Laboratorija za molekularnu biologiju je prva laboratorija u našoj zemlji koja je 1988. godine uvela molekularnu dijagnostiku cistične fibroze. U saradnji sa medicinskim ustanovama, obavljamo: postnatalnu i prenatalnu diganostiku cistične fibroze, kao i utvrđivanje statusa nosioca mutiranog CFTR gena ·prenatalnu dijagnostiku cistične fibroze ·postnatalnu dijagnostiku cistične fibroze ·utvrđivanje statusa nosioca Od 1996. naša laboratorija je uključena u Evropski program kontrole kvaliteta (European Quality Control Program) .U našoj labortoriji radi direktna detekcija najčešćih mutacija u CFTR genu: F508del, G551D, G542X, R553X, N1303K, 621+1G/T and 1717-1G/A. Pored toga, rutinski radimo i pretraživanje celog CFTR gena (27 egzona i susednih intronskih sekvenci). Obaveštenje za korisnike usluge CONTACT: Aleksandra Divac, Dr Mila Ljujic, Mr Dragica Radojkovic, Dr TEL: ++ 381 11 3976 658 FAX: ++ 381 11 3975 808 e-mail: qwert@eunet.rs Upravni odbor, Direktor, Zamenik direktora, Naučno veće, Laboratorije ... Predsednik Upravnog odbora, Sekretar za finansije ... Do nas možete doći autobuskim linijama : 33, 39 ili 25 ... Pogledajte naše slike ... Ministry of Science Belgrade University ICGEB more links ... "); array_files[32]=new Array(0,1,"http://www.imgge.bg.ac.yu/Srb/Services/trombophiliae.htm","2009-10-14","15K","IMGGI :: Trombofilija ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGI :: Trombofilija Pocetna > Komercijalne usluge RFLP analiza MTHFR C667T RFLP analiza FV Leiden PSM analiza FII G20210A U Laboratoriji za molekularnu biologiju je prvi put kod nas (1998. godine) uvedena molekularna dijagnostika trombofilije. Dijagnostički panel za trombofiliju obuhvata: detekciju FV Leiden, FII G20210A i MTHFR C677T mutacija. Indikacije za testiranje na ove mutacije su: lična i porodična anamneza venske tromboze, plućni embolizam, prisustvo APC rezistencije, cerebralni venski insult i periferna vaskularna bolest. Ove mutacije su takodje, značajni faktor rizika za pojavu ponovljenih spontanih pobačaja. Od 2004. godine učestvujemo u European Quality Control Program koji organizuje DGKL, Reference Institute for Bioanalytics. Obaveštenje za korisnike usluge CONTACT: Valentina Đorđević, Mr, Ljiljana Rakićević, Mr, Dragica Radojkovic, Dr TEL: ++ 381 11 3976 658 FAX: ++ 381 11 3975 808 e-mail: qwert@eunet.rs Upravni odbor, Direktor, Zamenik direktora, Naučno veće, Laboratorije ... Predsednik Upravnog odbora, Sekretar za finansije ... Do nas možete doći autobuskim linijama : 33, 39 ili 25 ... Pogledajte naše slike ... Ministry of Science Belgrade University ICGEB more links ... "); array_files[33]=new Array(0,4,"http://www.imgge.bg.ac.yu/Srb/Files/cf.pdf","2009-10-14","45K"," Microsoft Word - obavestenje za korisnike.doc ","","","Institut za molekularnu genetiku i geneticko inzenjerstvo Laboratorija za molekularnu biologiju Vojvode Stepe 444a, Beograd Tel: (011) 3976658 Fax: (011) 3975808 e-mail: qwert@eunet.yu Obavestenje za korisnike usluge ANALIZA MUTACIJA U CFTR GENU Laboratorije za molekularnu biologiju IMGGI Uzorak: Za geneticku analizu se koristi 5­10mL venske krvi uzete sa 3,8% Na citratom kao antikoagulansom u odnosu 9 : 1 (prilikom uzimanja krvi ne koristiti druge antikoagulanse). Na analize ne utice uzimanje lekova i hrane. Cena analize mutacija u CFTR genu: VRSTA ANALIZE utvr ivanje prisustva mutacije F508del utvr ivanje prisustva mutacija: F508del, G542X, G551D, R553X, N1303K, 621+1GT i 1717-1GA Pretrazivanje celog CFTR gena 1 (egzoni: 1, 3, 4, 5, 6a, 7, 9, 10, 11, 12, 13, 14a, 15, 17b, 19, 20, 21) Pretrazivanje celog CFTR gena 2 (egzoni:2, 6b, 8, 14b, 16, 17a, 18, 22, 23, 24) Prenatalna dijagnostika cisticne fibroze *U cenu je uracunat PDV Napomena: Prenatalna dijagnostika radi se iskljucivo uz prethodni dogovor i pod uslovom da su oba roditelja prethodno testirana. Nacin plaanja: Uplatu izvrsiti na ziro racun instituta CENA 7080din 14750din 59000din 35400din 11800din 105-1084-75 poziv na broj 9903DS sa naznakom za analizu mutacija u CFTR genu. Kontakt osoba: dr Dragica Radojkovi "); array_files[34]=new Array(0,4,"http://www.imgge.bg.ac.yu/Srb/Files/tromboza.pdf","2009-10-14","39K"," Microsoft Word - Document in Document1 ","","","Institut za molekularnu genetiku i geneticko inzenjerstvo Laboratorija za molekularnu biologiju Rukovodilac: Dr Snezana Koji, naucni saradnik Vojvode Stepe 444a, 11010 Beograd Obavestenje za korisnike usluga Instituta za molekularnu genetiku i geneticko inzenjerstvo Uzimanje krvi: Za geneticku analizu se koristi 5­10 ml venske krvi pacijenta uzete sa 3,8% Nacitratom kao antikoagulansom u odnosu 9 : 1 (prilikom uzimanja krvi ne koristiti druge antikoagulanse). Na analize ne utice uzimanje lekova i hrane. Krv se moze predati svakog radnog dana u periodu od 10-15 casova. Cena analiza: FV G1691A (Leiden mutacija) FII G20210A MTHFR C677T FV Leiden i FII G20210A FV Leiden i MTHFR C677T FII G20210A I MTHFR C677T FV Leiden, FIIG 20210A i MTHFR C677T U cenu je uracunat PDV, koji se plaa po stopi od 18%. Nacin plaanja: Uplatu izvrsiti na ziro racun instituta 6 490, 00 din 6 490, 00 din 6 490, 00 din 9 086, 00 din 9 086, 00 din 9 086, 00 din 15 576, 00 din 105-1084-75 Poziv na broj: 99 03DS Svrha doznake: za molekularnu dijagnostiku tromboze Preuzimanje rezultata: Vreme potrebno da se analize izvrse je dve do cetiri nedelje. Rezultati se mogu podii u periodu od 10-15 casova svakog radnog dana. Za dodatne informacije obratiti se nadleznoj osobi: mr Valentina or evi, molekularni biolog i fiziolog Laboratorija za molekularnu biologiju Telefon: Fax: e-mail: 011 / 397 66 58 011 / 397 58 08 qwert@eunet.yu "); array_files[35]=new Array(0,1,"http://www.imgge.bg.ac.yu/Services/thalassemia.htm","2009-10-14","14K","IMGGE :: Thalassemia ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGE :: Thalassemia Home > Commercial services The thalassemia syndromes are a heterogeneous group of inherited anemias characterized by defects in the synthesis of one or more of the globin chain subunits of the hemoglobin tetramer. The thalassemias are inherited as pathologic alleles of one or more of the globin genes. Clinical manifestations are diverse, ranging from asymptotic hypochromia and microcytosis to profound anemia, which is fatal in utero or in early childhood if untreated. When bone marrow transplantation is performed early in life there is a great probability of cure. The thalassemias are most common in the Mediterranean basin and equatorial or near-equatorial regions of Asia and Africa. FR Yugoslavia belongs to thalassemia belt where frequencies of pathologic genes are within the range of 2.5-15%. Molecular diagnostic of thalassemia is the most reliable method for: accurate diagnosis early in life. Modern therapy initiated in early stage prolongs survival of these patients. prenatal diagnosis, which decreases the incidence of thalassemia patient births. In Laboratory for molecular hematology the modern methods are used for diagnostic of thalassemia, including hemoglobin analysis (electrophoresis of Hb) and globin genes analysis ( reverse dot-blot, PCR). CONTACT: Dr Sonja Pavlović TEL: ++ 381 11 3976 445 FAX: ++ 381 11 3975 808 e-mail: sonya@sezampro.rs Organisational Chart Director, Chairman of the Board ... You can reach us by bus: 33, 39 or 25 ... See our history, events or take virtual tour photos ... Ministry of Science Belgrade University ICGEB more links ... "); array_files[36]=new Array(0,1,"http://www.imgge.bg.ac.yu/Srb/Services/aat.htm","2009-10-14","14K","IMGGI :: AAT ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGI :: AAT Pocetna > Komercijalne usluge Deficijencija alfa 1 antitripsina je autozomalno recesivno oboljenje koje je najčešći genetički uzrok oboljenja jetre kod dece i emfizema kod odraslih. Laboratorija za molekularnu biologiju je jedina laboratorija u našoj zemlji koja vrši molekularnu dijagnostiku deficijencije alfa 1 antitripsina detekcijom najčešćih mutiranih alela – Z i S. Testiranje je indikovano kod svih pacijenata sa sniženom koncentracijom alfa 1 antitripsina u plazmi, kod pacijenata sa emfizemom pluća kaoi kod pacijenata koji su u ranom dobu razvili hroničnu obstruktivnu bolest pluća ili oboljenje jetre. Od 2004. godine učestvujemo u European Quality Control Program koji organizuje DGKL, Reference Institute for Bioanalytics. CONTACT: Aleksandra Divac, PhD Mila Ljujić, Mr Dragica Radojkovic, Dr TEL: ++ 381 11 3976 658 FAX: ++ 381 11 3975 808 e-mail: qwert@eunet.rs Upravni odbor, Direktor, Zamenik direktora, Naučno veće, Laboratorije ... Predsednik Upravnog odbora, Sekretar za finansije ... Do nas možete doći autobuskim linijama : 33, 39 ili 25 ... Pogledajte naše slike ... Ministry of Science Belgrade University ICGEB more links ... "); array_files[37]=new Array(0,1,"http://www.imgge.bg.ac.yu/shorthistory.htm","2009-10-14","15K","IMGGE :: Short history ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGE :: Short history Home Institute of Molecular Genetics and Genetic Engineering (IMGGE - formerly Centre for Genetic Engineering) was founded in 1986 upon proclamation of the Law for the Ratification of Statute of the International Centre of Genetic Engineering and Biotechnology (ICGEB) by Yugoslav Parliament. Thus, the Institute is associated to ICGEB, Trieste, Italy, as an affiliated centre and it is involved in all activities within this international organization. The first director who actually fathered IMGGE was Professor Vladimir Glišin. The IMGGE has the collaboration with many institutes in the region as well as with the ICGEB member countries. In 1998 Institute moved to a new building of 2.400 m2 where most of the equipment necessary for sophisticated experimental manipulations in genetic engineering is available. Pursuant to the Decision of Serbian Government 05 No. 022-1183/2007 dated March 1st , 2007, IMGGE is established as a state research institute. The major activities of the Institute are oriented towards fundamental research in molecular biology, molecular genetics, recombinant DNA technology and biotechnology. In the field of fundamental research, Institutes projects are mostly focused on research related to analysis of genome organisation and regulation of gene expression in different organisms. Furthermore, IMGGE is also oriented to biotechnological and commercial applications in human and veterinary medicine, agriculture, food production, and pharmaceutical industry. The Institute consists of six laboratories: Laboratory for Molecular Genetics of Industrial Microorganisms, Laboratory for Molecular Genetics of Actinomycetes, Laboratory for Plant Molecular Biology, Laboratory for Human Molecular Genetics, Laboratory for Molecular Biology and Laboratory for Molecular Haematology. Staff of the Institute presently comprises 65 researchers. Apart from regular employees, IMGGE frequently hosts researchers from other research organisations. The Institute is affiliated to the University of Be "); array_files[38]=new Array(0,1,"http://www.imgge.bg.ac.yu/Laboratorije/05/lab.htm","2009-10-14","12K","IMGGE :: Laboratory for Molecular Genetics of Actinomycetes ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGE :: Laboratory for Molecular Genetics of Actinomycetes Home > Laboratories The research interest of Laboratory for Molecular Genetics of Actinomycetes is molecular genetics of Actinomycetes strains which produce secondary metabolites. Team members : Finished and ongoing projects, specific research goals ... List of Publications : List of Commercial Services : Ministry of Science Belgrade University ICGEB more links ... "); array_files[39]=new Array(0,1,"http://www.imgge.bg.ac.yu/Laboratorije/05/staff.htm","2009-10-14","15K","IMGGE :: Staff ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGE :: Staff Home > Laboratories > Laboratory for Molecular Genetics of Actinomycetes Head : Branka VASILJEVIĆ, PhD Associates : Ivana MORIĆ, PhD Miloje SAVIĆ, MSc Sandra VOJNOVIĆ, MSc Lidija ĐOKIĆ, BSc (PhD student) Tatjana ILIĆ TOMIĆ, MSc Tanja NARANČIĆ, BSc (PhD student) Nada STANKOVIĆ, MSc Sanja BAJKIĆ, BSc (PhD student) Finished and ongoing projects, specific research goals ... List of Publications : List of Commercial Services : Ministry of Science Belgrade University ICGEB more links ... "); array_files[40]=new Array(0,1,"http://www.imgge.bg.ac.yu/Laboratorije/05/publications.htm","2009-10-14","15K","IMGGE :: Publications ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGE :: Publications Home > Laboratories > Laboratory for Molecular Genetics of Actinomycetes Ilić – Tomić, T., Morić, I., Conn, G. L., Vasiljević, B.: Aminoglycoside resistance genes sgm and kgmB protect bacterial but not yeast small ribosomal subunits in vitro despite high conservation of the rRNA site. Res. in Microbiol. 159 (2008) 658 – 662. Savić, M., Ilić – Tomić, T., Macmaster, R., Vasiljević, B., Conn, G. L.: Critical Residues for Cofactor Binding and Catalytic Activity in the Aminoglycoside Resistance Methyltransferase Sgm. J. Bacteriol. 190 (2008) 5855 – 5861. Kojić, M., Vojnović, S., Vukov, N., Vasiljević, B.: Overexpression of Sgm 5’ UTR mRNA reduces gentamicin resistance in both Escherichia coli and Micromonospora melanosporea cells. Arch. Biol.Sci. 59 (2007) 273 – 280. Savić, M., Bratić I., Vasiljević, B.: Streptomyces durmitorensis sp. nov., a producer of an FK506-like immunosuppressant. International Journal of Systematic and Evolutionary Microbiology 57 (2007) 2119-2124. Savić, M., Vasiljević, B.: Targeting polyketide synthase gene pool within actinomycetes: new degenerate primers. J. Ind. Microbiol. Biotechnol. 33 (2006) 423-430. Marković, S., Vojnović, S., Jovanović, M., Vasiljević, B.: Different expression level of two KgmB-His fusion proteins. Journal of the Serbian Chemical Society 70 (2005) 1401-1407. Ilić Tomić, T., Marković, S., Vasiljević, B.: Expression and purification of the Sgm protein from E. coli. Journal of the Serbian Chemical Society 70 (2005) 817-822. Skoko, N., Vujović, J., Savić, M., Papić, N., Vasiljević, B., Ljubijankić, G.: Construction of Saccharomyces cerevisiae strain FAV20 useful in detection of immunosuppressants produced by soil actinomycetes. Journal of Microbiological Methods 61 (2005) 137-140. Vajić, S., Anastasov, N., Vasiljević, B.: The kgmB gene, encoding ribosomal RNA methylase from Streptomyces tenebrarius, is autogenously regulated. Archives of Microbiology. 182 (2004) 475-481. Kojić, M., Milojević, N., Vasiljević, B.: Gentamicin-resistance determi "); array_files[41]=new Array(0,1,"http://www.imgge.bg.ac.yu/Laboratorije/05/projects.htm","2009-10-14","16K","IMGGE :: Projects ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGE :: Projects Home > Laboratories > Laboratory for Molecular Genetics of Actinomycetes Projects: Structural and functional analysis of genes in industrial microorganisms. Molecular genetics of micromonospora (funded by MSTRS, 1996-2000). Regulation of gene expression in antibiotic producer  Streptomyces  tenebrarius (ICGEB/CRP/YUG00-05). Molecular genetics of microorganisms and expression of industrially important genes (funded by MSTRS, 1512, 2002-2004). Expression and regulation of pharmaceutically important genes in microorganisms (funded by MSEPRS, 143056, 2006-2010). Structure-function studies of 16S rRNA methyltransferases: the basis of aminoglycoside antibiotic resistance and enzyme translational autoregulation (funded by Wellcome Trust, 2006-2008). Search for new bioactive secondary metabolites (Bilateral cooperation project, Countries: Serbia, Czech Republic, 2006-2007). Molecular genetics of Actinomycetes strains which produce secondary metabolites Studying the mechanisms of resistance to aminoglycoside antibiotics. Cloning of the gene(s) conferring resistance to aminoglycoside antibiotics. Elucidation of expression and regulation of gene(s) involved in resistance. Interaction of aminoglycoside resistant methylases with 16S rRNA and with mRNA regulatory sequence(s). Forming actinomycetes collection isolated from different locations, phylogenetic analyses. Studying secondary metabolites. Studying of polyketide synthase (PKS) genes from actinomycetes with particular interest into PKSs involved in biosynthesis of immunosuppressive metabolites. List of Publications : Team members : List of Commercial Services : Ministry of Science Belgrade University ICGEB more links ... "); array_files[42]=new Array(0,1,"http://www.imgge.bg.ac.yu/Laboratorije/04/lab.htm","2009-10-14","12K","IMGGE :: Laboratory for Human Molecular Genetics ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGE :: Laboratory for Human Molecular Genetics Home > Laboratories The research interests of the Laboratory for Human Molecular Genetics include studying the regulation of expression and function of the human SOX genes and application of Fluorescent in situ Hybridization (FISH) for detection of numerical and structural chromosome aberrations in humans. Team members : Finished and ongoing projects, specific research goals ... List of Publications : List of Commercial Services : Ministry of Science Belgrade University ICGEB more links ... "); array_files[43]=new Array(0,1,"http://www.imgge.bg.ac.yu/Laboratorije/04/publications.htm","2009-10-14","15K","IMGGE :: Publications ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGE :: Publications Home > Laboratories > Laboratory for Human Molecular Genetics Petrović, I., Kovačević-Grujičić, N. and Stevanović, M. ZBP-89 and Sp3 down-regulate while NF-Y up-regulates SOX18 promoter activity in HeLa cells. 2008. Molecular Biology Reports, DOI 10.1007/s11033-008-9272-x. Kovačević Grujičić, N., Yokoyama, K. K. and Stevanović, M. Trans-activation of the human SOX3 promoter by MAZ in NT2/D1 cells. 2008. Archives of Biological Sciences, Belgrade, 60, 379-387. Nikčević, G., Savić, T., Kovačević-Grujičić, N. and Stevanović, M. Up-regulation of the SOX3 gene expression by retinoic acid: characterization of the novel promoter-response element and the retinoid receptors involved. 2008. Journal of Neurochemistry, 107, 1206-1215. Krstić, A., Mojsin, M., Kovačević-Grujičić, N. and Stevanović, M. PCR amplification and sequence analysis of the rat Sox3 gene. 2008. Archives of Biological Sciences, Belgrade, 60, 525-530. Djordjević, V., Dencić-Fekete, M., Jovanović, J., Drakulić, D., Stevanović, M., Janković, G. and Gotić, M. Pattern of trisomy 1q in hematological malignancies: a single institution experience. 2008. Cancer Genet Cytogenet. 186, 12-18. Cuturilo, G., Drakulic, D., Stevanovic, M., Jovanovic, I., Djukic, M., Miletic-Grkovic, S. and  Atanaskovic-Markovic, M. A rare association of interrupted aortic arch type C and microdeletion 22q11.2. 2008. Eur J Pediatr. 167, 1195-1198. Krstić, A., Mojsin, M. and Stevanović, M. Regulation of SOX3 gene expression is driven by multiple NF-Y binding elements. 2007. Archives of Biochemistry and Biophysics, 467, 163-173. Kovačević Grujičić, N., Mojsin, M., Djurović, J., Petrović, I. and Stevanović, M. Comparison of promoter regions of SOX3, SOX14 and SOX18 orthologs in mammals. 2008. DNA Sequence, 19, 185-194. Drakulić D., Nikčević, G., Djordjević, V. and Stevanović M. Generation of a whole chromosome painting probe from monochromosomal hybrid cells by the Alu -polymerase chain reaction.2007. Archives of Biological Sciences, 59, 89-95. Nikčević, G., Ko "); array_files[44]=new Array(0,1,"http://www.imgge.bg.ac.yu/Laboratorije/04/staff.htm","2009-10-14","16K","IMGGE :: Staff ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGE :: Staff Home > Laboratories > Laboratory for Human Molecular Genetics Head : Milena STEVANOVIĆ. PhD Associates : Gordana NIKČEVIĆ, PhD Jelena POPOVIĆ, MSc Marija MOJSIN, PhD Danijela DRAKULIĆ , MSc Andrijana KLAJN, PhD Isidora PETROVIĆ , MSc Nataša KOVAČEVIĆ GRUJIČIĆ , MSc Aleksandar KRSTIĆ , MSc Milena MILIVOJEVIĆ, BSc (PhD student) Finished and ongoing projects, specific research goals ... List of Publications : List of Commercial Services : Ministry of Science Belgrade University ICGEB more links ... "); array_files[45]=new Array(0,1,"http://www.imgge.bg.ac.yu/Laboratorije/04/projects.htm","2009-10-14","17K","IMGGE :: Projects ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGE :: Projects Home > Laboratories > Laboratory for Human Molecular Genetics Projects: Cloning and characterization of human SOX genes (funded by MSTRS, 1996-2000). Retinoic acid induction of the SOX3 gene (funded by The Wellcome Trust, London UK, 1996-2000). FISH (fluorescent in situ hybridization) and its application in prenatal diagnosis, detection of inherited diseases and in malignant transformation (funded by MSTRS, 1996-1997). Development and application of novel biotechnological methods and procedures for enlargement of production and improvement of the quality of meat, milk and eggs (funded by MSTRS, 1998-2000). Retinoic acid induction of the SOX3 gene expression (funded by ICGEB, 2000-2002). Human SOX genes: Studying the structure and regulation of expression (funded by MSEPRS, 2002-2004). Studying the regulation of expression and function of the human SOX genes (funded by MSRS, 2006-2010). Regulation of human SOX18 gene expression and its role in angiogenesis (funded by ICGEB, 2008-2010). Specifics of ongoing projects: Studying the regulation of expression and function of the human SOX genes SOX genes comprise the family of genes involved in regulation of differentiation and development in humans. By screening human fetal brain cDNA library and genomic library, we have cloned and characterized human SOX2 , SOX3 , SOX14 , SOX18 and SOX20 genes. Specific research goals: Studying the structure and transcriptional regulation of human SOX3 , SOX14 and SOX18 genes expression Detection of control elements and identification of transcription factors involved in the regulation of expression Investigation of retinoic acid induced activation of SOX3 gene expression in NT2/D1 cell line Studying the expression of human SOX genes in tumors of the neural origin by in situ hybridization Studying the control mechanisms responsible for transcriptional regulation of expression will contribute to the understanding of SOX gene functions in the control of development and differentiation. The research may contrib "); array_files[46]=new Array(0,1,"http://www.imgge.bg.ac.yu/Laboratorije/03/projects.htm","2009-10-14","20K","IMGGE :: Projects ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGE :: Projects Home > Laboratories > Laboratory for Molecular Biology 1. Genotype–phenotype correlation Given that interactions of numerous key players define a phenotype, every qualitative or quantitative change in gene expression could lead to its mild or severe modulation. For the genotype/phenotype studies we have chosen groups of genes involved in different types of hereditary disorders. These groups are relevant for respiratory (COPD, CF), haemostatic (thrombosis, hemophilia) and malignant (pancreatic adeno carcinoma, lung carcinoma) diseases. Involvement in host defense, inflammatory processes and protection against oxidative stress was common denominator for the majority of chosen genes. Our aim is to determine combined modulatory effect of different genes and to start the analysis of complex molecular events responsible for phenotype modulation. 2. Identification of mutations and polymorphisms in CFTR gene in CFTR related diseases Besides cystic fibrosis, the most frequent and severe genetic disease in Caucasians, the CFTR gene is involved in CFTR related disease. The involvement of CFTR gene mutations and polymorphisms in CFTRD (CBAVD, chronic pancreatitis and disseminated bronchiectasis) is investigated in patients with respective clinical diagnoses. 3. Application of molecular biology in medicine Prenatal and postnatal DNA diagnostics for mutations in CFTR, FII, FV, MTHFR, PAI and A1AT genes is performed and offered to medical institutions. 4. Eukaryotic DNA replication Binding of the initiator protein complex (ORC) to replicator DNA always initiates sequence of events leading to DNA replication. We have previously identified noncanonical DNA structures in functionally essential regions of the LMNB2 and DHFR origins of DNA replication. Formation of these structures was stimulated by HsOrc4 protein, one of the ORC subunits. Our aim is to investigate the ability of HsOrc4 to direct structural alterations of origin DNA when present in complex with different ORC subunits. In addition, we are t "); array_files[47]=new Array(0,1,"http://www.imgge.bg.ac.yu/Laboratorije/08/staff.htm","2009-10-14","17K","IMGGE :: Staff ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGE :: Staff Home > Laboratories > Laboratory for Plant Molecular Biology Head : Vesna MAKSIMOVIĆ, PhD Associates : Svetlana RADOVIĆ, PhD  Faculty of Biology,  University of Belgrade Bojana BANOVIĆ, BSc Miroslav KONSTANTINOVIĆ, PhD Živko JOVANOVIĆ, BSc Jovanka MILJUŠ ĐUKIĆ, PhD Leave of absence : Jelena SAMARDŽIĆ, MSc Jelena BRKLJAČIĆ, PhD Plant Biotech. Center,Columbus, USA Gordana TIMOTIJEVIĆ, MSc Nada ŠURBANOVSKI, MSc East Malling Research, UK Dragana NIKOLIĆ, MSc Ana BRATIĆ, BSc Karolinska Institute, Stockholm, Sweden Mira MILISAVLJEVIĆ, MSc Finished and ongoing projects, specific research goals ... List of Publications : List of Commercial Services : Ministry of Science Belgrade University ICGEB more links ... "); array_files[48]=new Array(0,1,"http://www.imgge.bg.ac.yu/Laboratorije/08/publications.htm","2009-10-14","15K","IMGGE :: Publications ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGE :: Publications Home > Laboratories > Laboratory for Plant Molecular Biology Ana M. Bratić, Dragana B. Majić, Jelena T. Samardžić and Vesna R. Maksimović, Functional analysis of the buckwheat metallothionein promoter: tissue specificity pattern and up-regulation under complex stress stimuli, J. Plant Phys. doi:10.1016/j.jplph.2008.12.002 2009 Bojana Banovic, Nada Surbanovski, Miroslav Konstantinovic, Vesna Maksimovic, Basic RNases of wild almond (Prunus webbii): Cloning and characterization of six new S-RNase and one ‘‘non-S RNase’’ genes, J. Plant Phys. 166, 395-402, 2008. M. Milisavljević, G. Timotijević, S. Radović, M. Konstantinović and V. Maksimović. Two types of aspartic proteinases from buckwheat seed–gene structure and expression analysis. J. Plant Phys. 165, 983-990 (2008) Nada Surbanovski, Kenneth R. Tobutt, Miroslav Konstantinovic, Vesna Maksimovic, Daniel J. Sargent, Vladimir Stevanovic, Encarnacion Ortega, Radovan I. Boskovic) Self-incompatibility of Prunus tenella and evidence that reproductively isolated species of Prunus have different SFB alleles coupled with an identical S-RNase allele. The Plant Journal 50, 723–734 (2007) Živko S. Jovanović , Jelena D. Milošević, Svetlana R. Radović Antioxidative enzymes in the response of buckwheat ( Fagopyrum esculentum Moench) to UV-B radiation , J. Agric. Food Chem., 54, 9472-9478(2006) Brkljačić J., Majić D., M iljuš -Đ ukić J., Bratić A., Konstantinović M., Milisavljevi ć M. and Vesna R. Maksimović The metallothionein-like gene from buckwheat – structural and functional analysis of the promoter region. Int. J. Plant Sci., 166: 889-900 (2005) Milisavljevic MD, Konstantinovic MM, Brkljacic JM, Maksimovic VR. Isolation and computer analysis of the 5 -regulatory region of the seed storage protein gene from buckwheat ( Fagopyrum esculentum Moench) J. Agric. Food Chem. 53: 2076-2080 (2005) Milisavljevic, M., Timotijević, G., Radović, S., Brkljačić, J., Konstantinović, M., Maksimović, V. Vicilin-like storage globulin from buckwheat seed. J. Agric. "); array_files[49]=new Array(0,1,"http://www.imgge.bg.ac.yu/Laboratorije/08/projects.htm","2009-10-14","20K","IMGGE :: Projects ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGE :: Projects Home > Laboratories > Laboratory for Plant Molecular Biology Current project Structure, Function and Regulation of Expression of Selected Plant Genes - basic research funded by Ministry of Science, Republic of Serbia (2006-2010). Previous projects Expression analysis of specific plant genes - a way to the function and biotechnological application - basic research funded by Ministry of Science, Republic of Serbia (2002-2005). Screening of the genetic diversity of wild species of Prunus in Serbia and Montenegro, and studying the molecular basis of self-incompatibility in wild species of Prunus incorporated in the project of basic research. Part of the project is being done in collaboration with East Malling Research, East Malling, UK (2002-2005). The improvement of technology of fruit and grape growing (applied research funded by Ministry of Science, Republic of Serbia, in collaboration with the Agricultural Research Institute Serbia and the Horticulture Research International, East Malling, UK (2002-2004). Development of the RT-PCR method for identification of RNA fruit viruses. Collaboration with the Agricultural Research Institute Serbia (from 1998-2001). Specifics of ongoing projects: 1. Analysis of selected plant genes and regulation of their expression The model-plant of the project is buckwheat. Analysis of the regulation of gene expression of specific buckwheat genes will be an element in the process of understanding common mechanism of regulation in a plant cells. It will also be a basis for elucidating possible functions of those proteins in different physiological processes and stress conditions in buckwheat. The main interest is the regulation of gene expression for two protein types: Metallothioneins - a chelator that accomplishes cellular metal homeostasis as well as redox homeostasis. Although MTs have been found in virtually all living organisms and their structure, localization and regulation of gene expression have been intensively studied, their specific functions in di "); array_files[50]=new Array(0,1,"http://www.imgge.bg.ac.yu/Laboratorije/08/lab.htm","2009-10-14","13K","IMGGE :: Laboratory for Plant Molecular Biology ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGE :: Laboratory for Plant Molecular Biology Home > Laboratories The main research interest is the study of selected genes encoding for proteins potentially involved in physiological processes that include protein degradation and/or stress response in plants - the aspartic proteinases (APs) and metallothionein (MT) from buckwheat. Studies of buckwheat tolerance and molecular mechanisms of response to various factors of abiotic stress (UV-B, drought, cold, NaCl, AlCl3 and heavy metals) also involve the role of antioxidative enzymes as well as non-enzymatic compounds. We also propose to investigate the main players of self-incompatibility system in buckwheat. Team members : Finished and ongoing projects, specific research goals ... List of Publications : List of Commercial Services : Ministry of Science Belgrade University ICGEB more links ... "); array_files[51]=new Array(0,1,"http://www.imgge.bg.ac.yu/Laboratorije/06/lab.htm","2009-10-14","12K","IMGGE :: Laboratory for Molecular Genetics of Industrial Microorganisms ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGE :: Laboratory for Molecular Genetics of Industrial Microorganisms Home > Laboratories The research interests of Laboratory for Molecular Genetics of Industrial Microorganisms include molecular genetics of Lactic Acid Bacteria (LAB) and molecular genetics of Pseudomonas. Team members : Finished and ongoing projects, specific research goals ... List of Publications : List of Commercial Services : Ministry of Science Belgrade University ICGEB more links ... "); array_files[52]=new Array(0,1,"http://www.imgge.bg.ac.yu/Laboratorije/06/staff.htm","2009-10-14","17K","IMGGE :: Staff ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGE :: Staff Home > Laboratories > Lab. for Molecular Genetics of Industrial Microorganisms Head : Ljubiša TOPISIROVIĆ, PhD Vice-Head: Ivana STRAHINIĆ, PhD Associates : Milan KOJIĆ, PhD Jelena BEGOVIĆ, PhD Đorđe FIRA, PhD Branko JOVČIĆ, MSc Nataša GOLIĆ, PhD Katarina VELJOVIĆ, MSc Amarela TERZIĆ VIDOJEVIĆ, PhD Milica NIKOLIĆ, MSc Jelena LOZO, PhD Maja TOLINAČKI, MSc Finished and ongoing projects, specific research goals ... List of Publications : List of Commercial Services : Ministry of Science Belgrade University ICGEB more links ... "); array_files[53]=new Array(0,1,"http://www.imgge.bg.ac.yu/Laboratorije/06/projects.htm","2009-10-14","21K","IMGGE :: Projects ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGE :: Projects Home > Laboratories > Lab. for Molecular Genetics of Industrial Microorganisms Projects: Molecular genetics of lactic acid bacteria (LAB) (funded by MSTRS, 2001-2005). Regulation of gene expression in stationary phase in Pseudomonas (Project No.: CRP/YUG02-01, funded by ICGEB, Trieste, Italy, 2003-2005) Study on the regulation of gene expression in relevant industrial microorganisms (funded by MSEPRS, Serbia, 2006-2010). Lactobacilles - changements des propriétés allergenes des produits laitiers fermentés et caracterisation des bactériocines (funded by Yugoslav-France Bilateral Scientific Collaboration “Pavle Savic”, 2006-2007). Development of technology for production of fermented goat’s milk products by using autochtonous LAB (funded by MSRS, 2007-2008). Use of lactic acid bacteria in production of hypoallergenic dairy products, and for the generation of mild antimicrobials (SEE-ERA.NET, reference number: 06-1000031-10522, 2007-2008) Molecular characterisation of bacteriocin producing strains (Project No.: CRP/YUG06-01, funded by ICGEB, Trieste, Italy, 2007-2009) Development of probiotic product by using specific lactic acid bacteria (funded by MSTDRS, 2008-2009). Specifics of ongoing projects: Lactic acid bacteria Collecting the natural isolates of LAB from traditional home-made fermented products as well as oral and vaginal lactobacilli. Construction of starter cultures for fermented food production at industrial scale. Characterisation of natural isolates producers of bacteriocins, exopolysaccharides, proteinases and searching for potential probiotics. Study on bacteriocin production by natural isolates of lactococci, enterococci and lactobacilli. Survey of the genes encoding proteinase in autochthonous lactic acid bacteria. Construction of suitable vectors for gene cloning and expression in LAB. Cloning and characterization of bacteriocin gene(s). Cloning and characterization of proteinase gene(s). Study on the regulation of gene expression in LAB. Pseudomonas sp. Characterization "); array_files[54]=new Array(0,1,"http://www.imgge.bg.ac.yu/Laboratorije/06/publications.htm","2009-10-14","16K","IMGGE :: Publications ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGE :: Publications Home > Laboratories > Lab. for Molecular Genetics of Industrial Microorganisms ben Omar, N., Valenzuela, A. S., Abriouel, H., López, R. L., Veljovic, K., Caňamero, M. M., Kojic, M., Topisirovic, L., Gálvez, A.: Virulence factors, antibiotic resistance, and bacteriocins in enterococci from artisan foods of animal origin. Food Control 20 (2009) 381-385. Strahinic, I., Kojic, M., Tolinacki, M., Fira, D. and Topisirovic, L. Molecular characterization of plasmids pS7a and pS7b from Lactococcus lactis subsp. lactis bv. diacetylactis S50 as a base for the construction of mobilizable cloning vectors. J. Appl. Microbiol. 106 (2009) 78-88. Lozo, J., Begovic, J., Jovcic, B., Golic, N., Topisirovic, L.: Effect of methionine and cysteine deprivation on the growth of different natural isolate of Lactobacillus spp. in chemically defined media. Arch. Biol. Sci. 60 (2008) 509-517. Nikolic, M., Terzic-Vidojevic, A., Jovcic, B., Begovic, J., Golic, N., Topisirovic, L.: Phenotypic and genotypic characterization of the microflora in the autochthonous homemade semi hard goats milk cheese. Int. J. Food Microbiol. 122 (2008) 162-170. Kojic, M., Jovcic, B., Begovic, J.,  Fira, D., Topisirovic, L.: Large chromosomal inversion correlated with spectinomycin resistance in Lactococcus lactis subsp. lactis bv. diacetylactis S50. Can. J. Microbiol. 54 (2008) 143-149. Busarcevic, M., Kojic M., Dalgalarrondo, M., Chobert, J. M., Haertlé, T., Topisirovic, L.: Purification of bacteriocin LS1 produced by human oral isolate Lactobacillus salivarius BGHO1. Oral. Microbiol. Immunol. 23 (2008) 254-258. Savic, D., Jokovic, N., Topisirovic, L.: Multivariate statistical methods in discrimination of lactobacilli based on their FTIR spectra. Dairy Sci. Tech. 88 (2008) 273-290. Jokovic, N., Nikolic, M., Begovic, J., Jovcic, B., Savic, D. and Topisirovic, L.: A survey of the lactic acid bacteria isolated from Serbian artisanal dairy product kajmak. Int. J Food Microbiol. 127 (2008) 305-311. Jovcic, B., Begovic, J., Lozo, J., Topi "); array_files[55]=new Array(0,1,"http://www.imgge.bg.ac.yu/Laboratorije/03/lab.htm","2009-10-14","12K","IMGGE :: Laboratory for Molecular Biology ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGE :: Laboratory for Molecular Biology Home > Laboratories The research interests of the Laboratory for Molecular Biology include genotype–phenotype correlation, identification of mutations i polymorphisms in CFTR gene in CFTR related diseases, application of molecular biology in medicine, study of skeletal muscle specific genes i DNA replication in eukaryotes. Team members : Finished and ongoing projects, specific research goals ... List of Publications : List of Commercial Services : Ministry of Science Belgrade University ICGEB more links ... "); array_files[56]=new Array(0,1,"http://www.imgge.bg.ac.yu/Laboratorije/03/staff.htm","2009-10-14","17K","IMGGE :: Staff ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGE :: Staff Home > Laboratories > Laboratory for Molecular Biology Head : Snežana KOJIĆ, PhD Professor Emeritus : Professor Ana SAVIĆ, PhD Associates : Dragica RADOJKOVIĆ, PhD, project leader Valentina ĐORĐEVIĆ, MSc Jelena KUŠIĆ, PhD Marija STANKOVIĆ,MSc Aleksandra DIVAC, PhD Branko TOMIĆ,MSc Ljiljana RAKIĆEVIĆ, MSc Mila LJUJIĆ, MSc Aleksandra NIKOLIĆ, MSc Aleksandra NESTOROVIĆ, MSc Dragana STEFANOVIĆ, PhD, retired Finished and ongoing projects, specific research goals ... List of Publications : List of Commercial Services : Ministry of Science Belgrade University ICGEB more links ... "); array_files[57]=new Array(0,1,"http://www.imgge.bg.ac.yu/Laboratorije/03/publications.htm","2009-10-14","16K","IMGGE :: Publications ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGE :: Publications Home > Laboratories > Laboratory for Molecular Biology M. Stankovic, A. Nikolic, A. Divac, A. Tomovic, N. Petrovic-Stanojevic, M. Andjelic, V. Dopudja-Pantic, M. Surlan, I. Vujicic, D. Ponomarev, M. Mitic-Milikic, J. Kusic,D. Radojkovic. The CFTR M470V gene variant as a potential modifier of COPD severity – study of Serbian population. Genet Test. 2008;12(3):357 Stefanovic D, Kusic J, Divac A, Tomic B. Formation of noncanonical DNA structures mediated by human ORC4, a protein component of the origin recognition complex Biochemistry. 2008 Aug 19;47(33):8760-7. Zaimidou S, van Baal S, Smith TD, Mitropoulos K, Ljujic M, Radojkovic D, Cotton RG, Patrinos GP. (2008) A(1)ATVar: a relational database of human SERPINA1 gene variants leading to alpha(1)-antitrypsin deficiency and application of the VariVis software. Hum Mutat. 2008 Nov 19. [Epub ahead of print] Radojkovic M, Ristic S, Colovic M, Cemerikic-Martinovic V, Radojkovic D, Krtolica K. Molecular characteristics and prognostic significance of Bcl-2/IgH gene rearrangement in Serbian follicular lymphoma patients. Neoplasma. 2008; 55(5):421-7. Castellani C, Cuppens H, Macek M Jr, Cassiman JJ, Kerem E, Durie P, Tullis E, Assael BM, Bombieri C, Brown A, Casals T, Claustres M, Cutting GR, Dequeker E, Dodge J, Doull I, Farrell P, Ferec C, Girodon E, Johannesson M, Kerem B, Knowles M, Munck A, Pignatti PF, Radojkovic D, Rizzotti P, Schwarz M, Stuhrmann M, Tzetis M, Zielenski J, Elborn JS. Consensus on the use and interpretation of cystic fibrosis mutation analysis in clinical     practice. J Cyst Fibros. 2008 May;7(3):179-96. Review. Nisevic I, Dinic J, Nikolic A, Djordjevic V, Lukic S, Ugljesic M, Andjelic-Jelic M, Petrovic-Stanojevic N, Radojkovic D. MTHFR C677T polymorphism in chronic pancreatitis and pancreatic adenocarcinoma. Cell Biochem Funct. 2008;26(6):659-63. Ljujic M, Topic A, Divac A, Nikolic A, Petrovic-Stanojevic N, Surlan M, Mitic-Milikic M, Radojkovic D. Isoelectric focusing phenotyping and denaturing gradient gel electrophor "); array_files[58]=new Array(0,1,"http://www.imgge.bg.ac.yu/Laboratorije/01/lab.htm","2009-10-14","12K","IMGGE :: Laboratory for Molecular Hematology ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGE :: Laboratory for Molecular Hematology Home > Laboratories The research interests of the Laboratory for Molecular Hematology include molecular genetics of leukemia, thalassemia syndromes, inflammatory bowel diseases and phenylketonuria, as well as pharmacogenetics and genetic diagnosis and molecular monitoring in the management of leukemia. Team members : Finished and ongoing projects, specific research goals ... List of Publications : List of Commercial Services : Ministry of Science Belgrade University ICGEB more links ... "); array_files[59]=new Array(0,1,"http://www.imgge.bg.ac.yu/Laboratorije/01/staff.htm","2009-10-14","17K","IMGGE :: Staff ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGE :: Staff Home > Laboratories > Laboratory for Molecular Hematology Head : Sonja PAVLOVIĆ, PhD, Senior Research Associate Associates : Tatjana KOSTIĆ, PhD, Research Associate Teodora KARAN ĐURAŠEVIĆ, BSc, Research Assistant Nataša TOŠIĆ, MSc, Research Assistant Milena RADMILOVIĆ, BSc, PhD student Milena Branka ZUKIĆ, BSc, Research Assistant Vesna SPASOVSKI , BSc, Research Trainee Maja STOJILJKOVIĆ, BSc, Research Assistant Mina GODEVAC, undergraduate student Nikola Kotur, undergraduate student Jelena ZARIĆ, PhD, Switzerland Aleksandra TRIFUNOVIĆ, PhD, Sweden Jelena JOVANOVIĆ, MSc, UK Jelena UROŠEVIĆ, MSc, Spain Finished and ongoing projects, specific research goals ... List of Publications : List of Commercial Services : Ministry of Science Belgrade University ICGEB more links ... "); array_files[60]=new Array(0,1,"http://www.imgge.bg.ac.yu/Laboratorije/01/publications.htm","2009-10-14","16K","IMGGE :: Publications ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGE :: Publications Home > Laboratories > Laboratory for Molecular Hematology Kollia P, Kalamaras A, Chassanidis C, Samara M, Vamvakopoulos NK, Radmilović M, Pavlović S, Papadakis MN, Patrinos GP. Compound heterozygosity for the Cretan type of non-deletional heriditary persistence of fetal hemoglobin and beta-thalassemia or Hb Sabine confirms the functional role of the A gamma -158 CT mutation in gamma-globin gene transcription. Blood Cells Mol Dis. 2008 Nov-Dec; 41(3):263-4. Protić MB, Pavlović ST, Bojić DZ, Krstić MN, Radojčić ZA, Tarabar DK, Stevanović AZ, Karan Đurašević TZ, Gođevac MV, Svorcan PV, Dapčević BD, Jojić NZ. CARD15 gene polymorphisms in Serbian patients with Crohn’s disease: genotype-phenotype analysis. Eur J Gastroenterol Hepatol. 2008 Oct; 20(10):978-84. Jevtović-Stojmenov T, Kocić G, Pavlović D, Macukanović-Golubovac L, Marjanović G, Đorđević V, Tošić N, Pavlović S. Polymorphisms of tumor-necrosis factor.alpha -308 and lymphotoxin-alpha +250: possible modulation of susceptibility to apoptosis in chronic lymphocytic leukemia and non-Hodgkin lymphoma mononuclear cells. Leuk Lymphoma. 2008 Nov; 49(11):2163-9. Stojiljkovic M, Stevanovic A, Djordjevic M, Petrucev B, Tosic N, Karan Djurasevic T, Aveic S, Radmilovic M, Pavlović S. Mutations in the PAH gene: a tool for population genetic study. Arch Biol Sci: 2007: 59 (3): 161-167. Colovic N, Tosic N, Aveic S, Djuric M, Milic N, Bumbasirevic V, Colovic M, Pavlovic S. Importance of early detection and follow-up of FLT3 mutations in patients with acute myeloid leukemia. Annals of Hematology 2007; 86: 741-747. 6.Jursic V, Terzic T, Pavlovic S, Colovic N, Colovic M. Elevated TNF-a and LDH without parathormone disturbance is associated with diffuse osteolytic lesions in leukemic transformation of myelofibrosis. Pathology Research and Practice. 2007. Oct30. Vesna Brankovic-Sreckovic, Vedrana Milic Rasic, Valentina Djordjevic, Milos Kuzmanovic, Sonja Pavlovic. Arterial Ischemic Stroke in a Child with ß-Thalassemia Trait and Methylentetrahydrofolat "); array_files[61]=new Array(0,1,"http://www.imgge.bg.ac.yu/Laboratorije/01/projects.htm","2009-10-14","13K","IMGGE :: Projects ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGE :: Projects Home > Laboratories > Laboratory for Molecular Hematology The hsp70 proteins in mutant cells. Collaboration project (funded by ICGEB, YUG97-04). Production of retroviral vector for application in gene therapy (MSTRS). Molecular diagnosis of thalassemia. Project: Molecular diagnosis of hereditary diseases in medicine (MSTRS). Mutated gene in different genetic backgrounds. (funded by MSEPRS, 2002-2004). Genomic elements in phenotype modulation (MSEPRS, 2005-2010) Clonal transformation of hematopoietic stem cells (MSEPRS, 2005-2010) Health Improvement in Serbia through Reinforcement of Biomedical Science and Technology “HISERBS” (EU, SSA, FP6-INCO-026357, 2006-2009) List of Publications : Team members : List of Commercial Services : Ministry of Science Belgrade University ICGEB more links ... "); array_files[62]=new Array(0,1,"http://www.imgge.bg.ac.yu/Vesti/janez/potocnik.htm","2009-10-14","14K","IMGGE : Visits ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGE : Visits Home On June 29th , 2009 Janez Potocnik, European Commissioner for Science and Research and Božidar Djelic, Minister of Science and Technological Development of the Republic of Serbia visited IMGGE, accompanied with: Josep M. Lloveras, Ambassador, Head of EU Delegation to the Republic of Serbia, Giancarlo Caratti, Head of Unit A, JRC European Commission, Tania Friederichs, JRC European Commission, Kristina Bole, European Commsission, Policy Assistant Aleksandar Djordjevic, Press and Information Officer, EU Delegation to the Republic of Serbia Organisational Chart Director, Chairman of the Board ... You can reach us by bus: 33, 39 or 25 ... See our history, events or take virtual tour photos ... Ministry of Science Belgrade University ICGEB more links ... "); array_files[63]=new Array(0,1,"http://www.imgge.bg.ac.yu/Services/interferon.htm","2009-10-14","15K","IMGGE :: Interferons ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGE :: Interferons Home > Commercial services Interferons are proteins able to mediate antiviral, anti-proliferative and immunomodulatory activities in response to a viral infection or other biological inducers. Recently an increasing number of clinical studies report on use of IFNß in successful treatment of multiple sclerosis (MS), viral hepatitis, human papilloma virus and in conventional fractionated radio therapy. On the world market there are two recombinant IFNß, one produced in bacteria E.coli (“BETASERON”, without first methionine and sugars and with one amino acid alteration) and the second in Chinese hamster ovary cells (“AVONEX”, high cost of cultivation). Betaseron costs approximately 1,000 for a monthly tretman of patients with relapsing-remitting MS ( 250µg/dose of Betaseron with a biological activity of 2xl0 7 IU/mg, patients receives 3 doses/week). Human IFNß gene was expressed in following expression systems: 1. E. coli (soluble, intracellular) – 10 6 U /l. 2. Saccharomyces cerevisiae ( episomal multi-copy expression vector ) Shake flask grown cells secretes recombinant human IFNß( rHuIFN- b ) into the medium at the level of 5x10 6 U/l (4). 3. P. pastoris (low- copy homologous integration ) rHuIFN- b was secreted from shake flask-grown P. pastoris cells into the medium at a level of 3x 10 8 U/l. This yield obtained in non optimised cultivation is higher by an order of magnitude than that obtained in the E. coli expression system. Biological activity was measured by inhibition of cytopathic effect of vesicular stomatitis virus on Wish cells. The ability of P. pastoris to grow to high densities in a fermentor together with the selection of rHuIFN- b multicopy P. pastoris clone would enable us even a larger production of functionally active rHuIFN- b . The primary structure of the rHuIFN beta (166 amino acids) is identical to that of natural human interferon beta, as determined by N-terminal amino acids sequencing. References: •  Todorović, V., Skoko, N., Pavković, N., Grujičić, N., Zarić, "); array_files[64]=new Array(0,1,"http://www.imgge.bg.ac.yu/Services/gmo.htm","2009-10-14","14K","IMGGE :: GMO ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGE :: GMO Home > Commercial services At the Laboratory for Plant Molecular Biology, the PCR METHOD for DETECTION, IDENTIFICATION and QUANTIFICATION OF GENETIC MODIFICATION IN PLANT MATERIAL AND FOOD/FEED PRODUCTS has been developed and standardized. The IMGGE is authorized by Ministry of Agriculture of Republic of Serbia as an official institution for GMO testing. The Laboratory is involved in International Quality Control system organized by International Seed Testing Association (ISTA proficiency test) Method comprises isolation of DNA and PCR tests: Qualitative a) General GMO screening (presence of CaMV35S promoter and NOS terminator)  b) Test specific for defined genetic modifications -RoundUp Ready soybean and corn NK603 (herbicide tolerant) -Liberty Link (T25) corn (herbicide tolerant) -Bt corn: Bt176-Bt11 and MON810 (insect resistant) Quantitative Real Time PCR test (Applied Biosystems, ABI Prism 7500) for quantification of genetic modification. METHOD IS ACCURATE AND SENSITIVE Detection limit is 1pg of DNA, enabling detection of genetic modification in: · samples of only 0.01% GMO (one modified out of 10,000) · food and feed products: lecithin, flour, chops, flakes, soybean meal, soybean protein isolates… The method is standardized according to criteria for accredited laboratories in Europe. CONTACT: Vesna Maksimović, Ph. D. TEL: ++ 381 11 3976 414 FAX: ++ 381 11 3975 808 e-mail: vesamax@imgge.bg.ac.rs Organisational Chart Director, Chairman of the Board ... You can reach us by bus: 33, 39 or 25 ... See our history, events or take virtual tour photos ... Ministry of Science Belgrade University ICGEB more links ... "); array_files[65]=new Array(0,1,"http://www.imgge.bg.ac.yu/Services/fish.htm","2009-10-14","13K","IMGGE :: FISH ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGE :: FISH Home > Commercial services Detection of 22q11.2 micro-deletion (DiGeorge syndrome) FISH is applied in prenatal and postnatal detection of chromosome aberrations in humans for: Diagnostics of micro-deletion syndromes (DiGeorge and Prader-Willi / Angelman) Detection of the most common numerical chromosomal aberrations (chromosomes 13, 18, 21, X and Y) Marker chromosomes detections Detection of rare mosaic karyotypes CONTACT: Milena Stevanovic, PhD TEL: ++ 381 11 3976 212 FAX: ++ 381 11 3975 808 e-mail: stevanov@eunet.rs Organisational Chart Director, Chairman of the Board ... You can reach us by bus: 33, 39 or 25 ... See our history, events or take virtual tour photos ... Ministry of Science Belgrade University ICGEB more links ... "); array_files[66]=new Array(0,1,"http://www.imgge.bg.ac.yu/Services/cf.htm","2009-10-14","14K","IMGGE :: Cystic Fibrosis ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGE :: Cystic Fibrosis Home > Commercial services DGGE analysis of CFTR exon 12 Laboratory for Molecular Biology is the first one in our country that in 1988. introduced molecular diagnostics of cystic fibrosis and CFTR gene mutation detection (Cystic fibrosis mutation database). In collaboration with medical centers, we performpostnatal and prenatal molecular genetic testing of cystic fibrosis in at risk families, as well as carrier testing. Since 1996, our laboratory has been participating in the European Quality Control Program. We perform testing for the most common CFTR mutations: F508del, G551D, G542X, R553X, N1303K, 621+1G/T and 1717-1G/A. We also routinely perform screening of the whole CFTR gene (27 exons with flanking intronic sequences). CONTACT: Aleksandra Divac, PhD Mila Ljujic, MSc Dragica Radojkovic, PhD TEL: ++ 381 11 3976 658 FAX: ++ 381 11 3975 808 e-mail: qwert@eunet.rs Organisational Chart Director, Chairman of the Board ... You can reach us by bus: 33, 39 or 25 ... See our history, events or take virtual tour photos ... Ministry of Science Belgrade University ICGEB more links ... "); array_files[67]=new Array(0,1,"http://www.imgge.bg.ac.yu/Services/blad.htm","2009-10-14","14K","IMGGE :: BLAD ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGE :: BLAD Home > Commercial services s) standard Phi X/HaeIII k) undigested PCR product z) healthy animal h) heterozygous carrier (BLAD) is a lethal autosomal recessive disease of Holstein-Friesian cattle. Affected calves suffer from frequent infections, which either fail to respond to, or recur, after conventional treatment. They normally die before reaching sexual maturity. The cause of the immunodeficiency is a point mutation in the bovine CD18 gene. To detect normal and defective CD18 allele we have developed a DNA test based on polymerase chain reaction (PCR) followed by restriction enzyme digestion. This test has been used to identify affected (BLAD) and normal (z) animals as well as heterozygous carriers (h). Molecular diagnostics of BLAD has been applied to exclude heterozygous carriers from breeding program. CONTACT: Jelena Popovic, MSc TEL: ++ 381 11 3976 212 FAX: ++ 381 11 3975 808 e-mail: jpopovic@imgge.bg.ac.rs Organisational Chart Director, Chairman of the Board ... You can reach us by bus: 33, 39 or 25 ... See our history, events or take virtual tour photos ... Ministry of Science Belgrade University ICGEB more links ... "); array_files[68]=new Array(0,1,"http://www.imgge.bg.ac.yu/Services/aat.htm","2009-10-14","13K","IMGGE :: AAT ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGE :: AAT Home > Commercial services Alpha -1-antitrypsin (A1AT) deficiency is an autosomal recessive disorder that is most common genetic cause of liver disease in children and emphysema in adults. The Laboratory for Molecular Biology is the onlylaboratory in our country performing molecular diagnostics of alpha-1-antitrypsin deficiency by detection of the two most common mutated alleles – Z and S. All patients with decreased plasma level of A1AT protein are indicated for testing, as well as those who develop pulmonary emphysema, chronic obstructive pulmonary disease (COPD) or liver disease at young age. Since 2004 our laboratory has participated in European Quality Control Program organized by DGKL, Reference Institute for Bioanalytics. CONTACT: Aleksandra Divac, PhD Mila Ljujić, MSc Dragica Radojkovic, PhD TEL: ++ 381 11 3976 658 FAX: ++ 381 11 3975 808 e-mail: qwert@eunet.rs Organisational Chart Director, Chairman of the Board ... You can reach us by bus: 33, 39 or 25 ... See our history, events or take virtual tour photos ... Ministry of Science Belgrade University ICGEB more links ... "); array_files[69]=new Array(0,1,"http://www.imgge.bg.ac.yu/Services/trombophiliae.htm","2009-10-14","15K","IMGGE :: Trombophiliae ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGE :: Trombophiliae Home > Commercial services RFLP analysis of MTHFR C667T RFLP analysis of FV Leiden PSM analysis of FII G20210A Laboratory for Molecular Biology is the first laboratory in our country which in 1998 introduced molecular diagnostics of thrombophilia. The thrombophila diagnostic panel includes: FV Leiden, FII G20210A and MTHFR C677T mutations. Indications for testing are: personal or family history of venous thrombosis, pulmonary embolism, APC resistance, cerebral-vein thrombosis and periferal vascular disease. These mutations are also significant risk factors for unexplained recurrent miscarriages. Since 2004. our laboratory has participated in the European Quality Control Program organized by DGKL, Reference Institute for Bioanalytics. CONTACT: Valentina Đorđević, MSc, Ljiljana Rakićević, MSc, Dragica Radojkovic, PhD TEL: ++ 381 11 3976 658 FAX: ++ 381 11 3975 808 e-mail: qwert@eunet.rs Organisational Chart Director, Chairman of the Board ... You can reach us by bus: 33, 39 or 25 ... See our history, events or take virtual tour photos ... Ministry of Science Belgrade University ICGEB more links ... "); array_files[70]=new Array(0,1,"http://www.imgge.bg.ac.yu/Services/tptm.htm","2009-10-14","14K","IMGGE :: TPMT ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGE :: TPMT Home > Commercial services Thiopurine S-methyltransferase (TPMT) is a cytosolic enzyme that catalyzes S-methylation (inactivation) of thiopurine drugs such as anticancer (6-mercaptopurine and 6-thioguanine) and immunosuppressant agents (azathioprine). These drugs are widely used for treatment of: 1. cancers 2. leukemias 3. autoimmune diseases 4. inflammatory bowel disease (ulcerous colitis, Crohns disease) Decreased activity of TPMT is associated with severe hematopoietic toxicity after using standard doses of these drugs. Moreover, this toxicity can be fatal. There are several mutations in TPMT gene which give rise to low phenotypic activity. The doses of administered thiopurine drugs are reduced regarding patients genotype character. After that, patients do not develop hematopoietic toxicity complications. Mutations detected in the Laboratory for Molecular Hematology by DNA analysis are: TPMT(G238C), TPMT (G460A) and TPMT (A719G). CONTACT: Dr Sonja Pavlović TEL: ++ 381 11 3976 445 FAX: ++ 381 11 3975 808 e-mail: sonya@sezampro.rs Organisational Chart Director, Chairman of the Board ... You can reach us by bus: 33, 39 or 25 ... See our history, events or take virtual tour photos ... Ministry of Science Belgrade University ICGEB more links ... "); array_files[71]=new Array(0,1,"http://www.imgge.bg.ac.yu/Services/starter.htm","2009-10-14","18K","IMGGE :: Starter Cultures ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGE :: Starter Cultures Home > Commercial services A large collection of natural isolates of lactic acid bacteria (LAB) was made mostly from different homemade cheeses and fermented milk products that are produced in very specific ecologically isolated regions throughout Serbia. These regions such as, for instance, the high mountains, the river valleys, the seacoast, etc. are characterized by extremely clean surrounding. In addition, people inhabiting some of these regions are long living population. All strains have been biochemically and genetically characterized in the Institute for Molecular Genetics and Genetic Engineering , Belgrade, Serbia . On the basis of this research, the various starter cultures containing precisely characterized LAB were constructed for production of cheeses and fermented milk products. These starter cultures were tested on industrial scale in dairy plants in Serbia. 1. ACIDOVIT PRODUCTION OF “ACIDOVIT” “Acidovit” is low fat, dietary fermented milk product made from the skimmed pasteurized milk. Manufacturing of “Acidovit” is possible in the standard yoghurt production line without any substantial changes or significant investments. For production of “Acidovit”, the only addition to the yoghurt production line is a vat with cooling system. The final product is dietary fermented milk beverage with 0.1% milk fat, 0.5% of minerals, 3.9% of protein and 5% of lactose (total dry mater of 9.5%). Energy value of “Acidovit” is 151.58 kJ/100gr or 312.57 kJ per 0.2 litre package. Except the good nutritive characteristics typical for all fermented milk beverages, “Acidovit” also has some additional properties because of the presence of Lactobacillus helveticus . It has strong antimicrobial activity and inhibits the growth of pathogenic and food spoilage microorganisms, such as bacteria from genus Escherichia, Proteus, Pseudomonas, Bacillus and Clostridium . “Acidovit” could be manufactured in two versions, according to the taste of consumers. A) ”ACIDOVIT” from pasteurized skim milk with "); array_files[72]=new Array(0,1,"http://www.imgge.bg.ac.yu/Services/porcine.htm","2009-10-14","14K","IMGGE :: Porcine ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGE :: Porcine Home > Commercial services s) standard Phi X/HaeIII k) undigested PCR product T/T) affected animal C/T) heterozygous carrier C/C) healthy animal Porcine stress syndrome (Malignant hyperthermia) is a devastating, a potentially lethal response to anesthetics that occurs in genetically predisposed individuals. Malignant hyperthermia triggered by stress leads to sudden death of animals resulted by pale, soft and exudative pork meat. A mutation at position 1843 has been discovered in the ryanodine-receptor gene (RYR1) causing malignant hyperthermic syndrome in pigs. The substitution of T to C at that position in RYR1 receptor is changing a restriction site, enabling the PCR-RFLP test to be performed in order to identify animals homo and heterozygous for this mutation. This test has been used to identify affected (T/T) and normal (C/C) animals as well as heterozygous carriers (C/T). Molecular diagnostics of porcine stress syndrome has been applied to exclude heterozygous carriers from breeding program. CONTACT: Jelena Popovic, MSc TEL: ++ 381 11 3976 212 FAX: ++ 381 11 3975 808 e-mail: jpopovic@imgge.bg.ac.rs Organisational Chart Director, Chairman of the Board ... You can reach us by bus: 33, 39 or 25 ... See our history, events or take virtual tour photos ... Ministry of Science Belgrade University ICGEB more links ... "); array_files[73]=new Array(0,1,"http://www.imgge.bg.ac.yu/Services/sry.htm","2009-10-14","13K","IMGGE :: SRY ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGE :: SRY Home > Commercial services s) standard Phi X/HaeIII k) negative control ?) sex reversed patients (46, XY females) ž) female positive control m) male positive control PCR based method is applied for detection of sex determining gene SRY in patients with sexual development disorders. Accurate and precise diagnostics and genetic counselling are aimed to prevent malignant transformation in sex reversal patients. CONTACT: Milena STEVANOVIĆ, Dr. TEL: ++ 381 11 3976 212 FAX: ++ 381 11 3975 808 e-mail: stevanov@eunet.rs Organisational Chart Director, Chairman of the Board ... You can reach us by bus: 33, 39 or 25 ... See our history, events or take virtual tour photos ... Ministry of Science Belgrade University ICGEB more links ... "); array_files[74]=new Array(0,1,"http://www.imgge.bg.ac.yu/Services/pku.htm","2009-10-14","13K","IMGGE :: PKU ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGE :: PKU Home > Commercial services Phenylketonuria (PKU) is the most common inborn error of amino acid metabolism in Caucasians. It is caused by an autosomal recessive deficiency of the hepatic enzyme phenylalanine hydroxylase (PAH). Inability of phenylalanine conversion to tyrosine results in an increase of phenylalanine in body fluids and severe mental retardation unless the dietary phenylalanine intake is restricted. In the Laboratory for Molecular Hematology testing for PKU is done by DNA analysis. Identifying the disease-causing mutation is useful for: refining diagnosis anticipating dietary requirements genetic counselling CONTACT: Dr Sonja Pavlović TEL: ++ 381 11 3976 445 FAX: ++ 381 11 3975 808 e-mail: sonya@sezampro.rs Organisational Chart Director, Chairman of the Board ... You can reach us by bus: 33, 39 or 25 ... See our history, events or take virtual tour photos ... Ministry of Science Belgrade University ICGEB more links ... "); array_files[75]=new Array(0,1,"http://www.imgge.bg.ac.yu/Services/penicillin.htm","2009-10-14","16K","IMGGE :: Penicillin ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGE :: Penicillin Home > Commercial services Penicillin G amidase (E.C. 3.5.1.11; PAC) catalyses the hydrolysis of the production of semisynthetic beta-lactam antibiotics. It is one of the most widely used enzymes at an the acyl group of the penicillin G, yielding 6-aminopenicillanic acid (6-APA), a key intermediate in industrial scale whose market is immediately after glucose isomerase. The world manufacturing of the immobilized enzyme is around 10 tone per year and 7500 tone of 6APA. Production of 1kg 6APA (1500 ) needs 1kg of penicillin (10 ) and 2,8g of enzyme penicillin G amidase (650 ). The P.rettgeri pac gene was expressed in following expression systems: Saccharomyces cerevisiae (episomal multi-copy expression vector) Shake flask culture produce ~ 1.5U/ml (1). The enzyme is secreted into the medium. Batch fermentation expression level is 5.7U/ml. The PAC yield is significantly increased by more than 500-fold compared to that obtained in the native bacterium, and the recombinant enzyme is almost entirely secreted (2). Recombinant PAC is purified by a combination of hydrophobic interaction and ion-exchange chromatography. •  Pichia pastoris (integration expression vector) , low and multi-copy homologous integration, produce 0.18U/ml (3) and 2.4U/ml respectively in shake flask culture in non optimised conditions. Recombinant PAC produced in P.pastoris is highly thermostable compared to the one produced in S.cerevisiae. References: •  Ljubijankić, G., Konstantinović, M. and Glišin, V. 1992. The primary structure of Providencia rettgeri penicillin G amidase gene and its relationship to other Gram negative amidases. DNA Sequence-J. DNA Sequencing and Mapping 3: 195-200. •  Konstantinović, M., Marjanović, N., Ljubijankić, G. and Glišin, V. 1994. The penicillin amidase of Arthrobacter viscosus (ATCC 15294). Gene 143: 79-83. •  Carlo Bruschi and Goran Ljubijankić, 1995. The method for the production of Penicillin G amidases in yeast Saccharomyces cerevisiae , Italian patent application MI95-A001083. •  Lju "); array_files[76]=new Array(0,1,"http://www.imgge.bg.ac.yu/Services/pcr.htm","2009-10-14","14K","IMGGE :: PCR ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGE :: PCR Home > Commercial services k-CASEIN s) standard Phi X/HaeIII k) PCR product AB) heterozygous AA) homozygous for variant A BB) homozygous for variant B ß- LACTOGLOBULIN s) standard Phi X/HaeIII k) PCR product AB) heterozygous AA) homozygous for variant A BB) homozygous for variant B PCR and subsequent restriction fragment length polymorphism analysis (PCR-RFLP) have been used for genotyping milk protein variants. Accurate genotyping of k-casein and b-lactoglobulin and selection of desirable genotypes have economic significance since their genetic variants have effects on milk production traits. CONTACT: Jelena Popovic, MSc TEL: ++ 381 11 3976 212 FAX: ++ 381 11 3975 808 e-mail: jpopovic@imgge.bg.ac.rs Organisational Chart Director, Chairman of the Board ... You can reach us by bus: 33, 39 or 25 ... See our history, events or take virtual tour photos ... Ministry of Science Belgrade University ICGEB more links ... "); array_files[77]=new Array(0,1,"http://www.imgge.bg.ac.yu/Services/patogenic.htm","2009-10-14","13K","IMGGE :: Pathogenic ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGE :: Pathogenic Home > Commercial services Molecular diagnostics of the following microorganisms is established: •  Listeria monocytogenes •  Yersinia enterocolitica •  Helicobacter pylori •  Mycoplasma pneumoniae •  Chlamidia trachomatis •  Chlamidia pneumonie •  Campilobacter jejuni •  Borrelia burgdoferi • Brucella sp CONTACT: Prof. Ljubiša Topisirović, PhD TEL: ++ 381 11 3975 960 FAX: ++ 381 11 3975 808 e-mail: lab6@eunet.rs Organisational Chart Director, Chairman of the Board ... You can reach us by bus: 33, 39 or 25 ... See our history, events or take virtual tour photos ... Ministry of Science Belgrade University ICGEB more links ... "); array_files[78]=new Array(0,1,"http://www.imgge.bg.ac.yu/fondation.htm","2009-10-14","13K","IMGGE : Foundation ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGE : Foundation Home In the memory of Dr. Goran Ljubijankić, the Foundation named “Goran Ljubijankić” was established on July 1, 2003. The Foundation is located in the Institute of Molecular Genetics and Genetic Engineering, Belgrade, where Goran spent the most productive years of his scientific research. The establishment of the Foundation, in addition to Gorans parents, Danica and Rasim Ljubijankić, and his home Institute, was helped by his colleagues form the International Centre for Genetic Engineering and Biotechnology, Trieste, Italy. The aim of the Foundation is to give support to the young and promising scientists from our country in the field of Molecular Biology. Foundation “Goran Ljubijankić” will give awards every January 30, on Gorans birthday, to the most outstanding young researchers in the field of Molecular Biology.   Organisational Chart Director, Chairman of the Board ... You can reach us by bus: 33, 39 or 25 ... See our history, events or take virtual tour photos ... Ministry of Science Belgrade University ICGEB more links ... "); array_files[79]=new Array(0,1,"http://www.imgge.bg.ac.yu/festival.htm","2009-10-14","13K","IMGGE : Festival ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGE : Festival Home At the Science Festival, held from 5th to 7th December 2008, IMGGE organized a prize draw as a part of the «Secrets of the DNA» stand. Around 1550 visitors attacked the task to translate DNA sequence into an amino acid sequence of the protein. Ten lucky winners visited our Institute on January, 22nd 2009. They spent some time with our researchers and did some experiments. They extracted DNA from their own buccal swabs, analyzed DNA on agarose gel and took it home as a souvenir. Also, they saw what researcher do at the Institute, how the bacteria are coloured and what do they look under the microscope, how DNA is labelled with fluorescent colours and how it’s analyzed, how to grow plant and mammalian cells in cell culture. (pictures ...) 10 lucky winners: Irma Bosna Matija Veljković Nikola Glavonjić Dunja Damjanović Ksenija Dangubić Marko Đorđević Marko Karanović Aleksa Ratarac Mihajlo Stošić Ana Todorović Organisational Chart Director, Chairman of the Board ... You can reach us by bus: 33, 39 or 25 ... See our history, events or take virtual tour photos ... Ministry of Science Belgrade University ICGEB more links ... "); array_files[80]=new Array(0,1,"http://www.imgge.bg.ac.yu/equipment.htm","2009-10-14","17K","IMGGE : Equipment ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGE : Equipment Home DNA sequencing and fragment analysis Applied Biosystems 3130 Genetic Analyzer is fully automated, fluorescence-based 4-capillary electrophoresis instrument. Two main applications of this instrument are DNA sequencing and DNA fragment analysis, but it can also be used for various other fragment analysis-based applications (LOH, MLPA, DGGE, SSCP, MCA, AFLP, SNPlex, SNaPshot). Data acquired by sequencing are processed by Sequencing Analysis Software, SeqScape Software and MicroSeq ID Analysis Software, while GeneMapper Software enables basic processing of data acquired by fragment analysis-based applications. Real-time PCR Applied Biosystems 7500 “Real–time” PCR System can perform all major real-time PCR applications including quantitative measurement of mRNA (absolute or relative quantification of gene expression), detection of single nucleotide polymorphisms (SNPs) and detection of the presence or absence of a specific target sequence (Plus/minus assays). Agilent 2001 bioanalyzer, lab-on-a-chip The Agilent 2100 Bioanalyzer is a microfluidics-based platform for the analysis of DNA, RNA, proteins and cells. It utilizes micro-fabricated chips, requiring minimum sample consumption and delivering automated, high quality digital data within 30 minutes. Macromolecules separation by centrifugation Beside classical laboratory Sorvall centrifuges (Du Pont Instruments), microcentrifuges (Eppendorf) with or without cooling systems, there are also several ultracentrifuges in IMGGE. Optima L-80 XP is a new, enhanced preparative ultracentrifuge instrument, capable of generating 602 000g at speed of up to 80 000 rpm. Centrifugation parameters are optimized by Optima eXPert Software. Environmentally-friendly cooling systems eliminate the need for CFCs. DNA and protein visualization systems Biometra`s BioDocAnalyse system, containing very light sensitive CCD camera for image acquisition, is used for high performance gel documentation and gel analysis. Cyclone Storage Phosphor System (Perkin Elmer)performs "); array_files[81]=new Array(0,1,"http://www.imgge.bg.ac.yu/copyright.htm","2009-10-14","14K","IMGGE : About ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGE : About Institute of Molecular Genetics and Genetic Engineering (IMGGE - formerly Centre for Genetic Engineering) was founded in 1986. The Institute is associated to International Center for Genetic Engineering and Biotechnology (ICGEB), Trieste, Italy, as an affiliated centre and it is involved in all activities within this international organization. In the field of fundamental research, Institutes projects are mostly focused on research related to analysis of genome organisation and regulation of gene expression in different organisms. Furthermore, IMGGE is also oriented to biotechnological and commercial applications in human and veterinary medicine, agriculture, food production, and pharmaceutical industry. Foundation “Goran Ljubijankić” Representatives of the World Bank visited IMGGI Science Festival, 5th - 7th December 2008 ICGEB funding programmes See our history, events or take virtual tour photos ... IMGGE (formerly Center for Genetic Engineering) was founded in 1986 ... You can reach us by bus: 33, 39 or 25 ... Ministry of Science Belgrade University ICGEB more links ... "); array_files[82]=new Array(0,1,"http://www.imgge.bg.ac.yu/board.htm","2009-10-14","13K","IMGGE :: Administration ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGE :: Administration Home ACTING PRESIDENT : Prof. Pavle MILENKOVIĆ, PhD ACTING MEMBERS : Miodrag PETROVIĆ , PhD Snežana PAJOVIĆ , PhD Prof. Slavica SPASIĆ , PhD Milena STEVANOVIĆ , PhD Gordana NIKČEVIĆ, PhD Ivana STRAHINIĆ, PhD Vesna MAKSIMOVIĆ, PhD Organisational Chart Director, Chairman of the Board ... You can reach us by bus: 33, 39 or 25 ... See our history, events or take virtual tour photos ... Ministry of Science Belgrade University ICGEB more links ... "); array_files[83]=new Array(0,1,"http://www.imgge.bg.ac.yu/administration.htm","2009-10-14","14K","IMGGE :: Administration ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGE :: Administration Home DIRECTOR: Branka VASILJEVIĆ, PhD Tel: (+381 11) 3975 744 E-mail: vasiljb@eunet.rs ACTING CHAIRMAN OF THE BOARD: Pavle MILENKOVIĆ, PhD Tel: (+381 11) 2684 484 E-mail: mpavle@imi.bg.ac.rs CHAIRMAN OF THE SCIENTIFIC COUNCIL: Vesna MAKSIMOVIĆ, PhD Tel: (+381 11) 3976 414 E-mail: heljda@sezampro.rs SECRETARY: Violeta EVTOV Tel: (+381 11) 3975 744 E-mail: beogene@eunet.rs FINANCE: Duško VUJIĆ Maja JEVTIĆ Tel: (+381 11) 3976 065 E-mail: imggi@sezampro.rs FAX : (+381 11) 3975 808 IMGGE (formerly Center for Genetic Engineering) was founded in 1986 ... Organisational Chart You can reach us by bus: 33, 39 or 25 ... See our history, events or take virtual tour photos ... Ministry of Science Belgrade University ICGEB more links ... "); array_files[84]=new Array(0,1,"http://www.imgge.bg.ac.yu/usefullinks.htm","2009-10-14","13K","IMGGE :: Useful links ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGE :: Useful links Home Ministry of Science Belgrade University Faculty of Biology National Library of Serbia University Library Svetozar Markovic International Center for Genetic Engineering and Biotechnology - ICGEB EUROGENTEST CF Network NIZO European Plant Science Organization The Serbian Mutation Frequency database ORPHANET Organisational Chart Director, Chairman of the Board ... You can reach us by bus: 33, 39 or 25 ... See our history, events or take virtual tour photos ... "); array_files[85]=new Array(0,1,"http://www.imgge.bg.ac.yu/scientific.htm","2009-10-14","15K","IMGGE :: Scientific coucncil ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGE :: Scientific coucncil Home Members of the Scientific Council: •  Vesna MAKSIMOVIĆ, PhD •  Milena STEVANOVIĆ, PhD •  Branka VASILJEVIĆ, PhD •  Dragica RADOJKOVIĆ, PhD •  Miroslav KONSTANTINOVIĆ, PhD •  Milan KOJIĆ, PhD •  Gordana NIKČEVIĆ, PhD •  Jovanka MILJUŠ ĐUKIĆ, PhD •  Nataša GOLIĆ, PhD •  Ivana STRAHINIĆ, PhD •  Sonja PAVLOVIĆ, PhD •  Amarela TERZIĆ VIDOJEVIĆ, PhD •  Snežana KOJIĆ, PhD •  Jelena KUŠIĆ, PhD •  Ivana MORIĆ, PhD Organisational Chart Director, Chairman of the Board ... You can reach us by bus: 33, 39 or 25 ... See our history, events or take virtual tour photos ... Ministry of Science Belgrade University ICGEB more links ... "); array_files[86]=new Array(0,1,"http://www.imgge.bg.ac.yu/reachus.htm","2009-10-14","13K","IMGGE :: Our Location ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGE :: Our Location Home You can reach us by bus: 33, 39 or 25 GSP Beograd PlanPlus Also, you can easyly reach us by car. See the enclosed map. Organisational Chart Director, Chairman of the Board ... You can reach us by bus: 33, 39 or 25 ... See our history, events or take virtual tour photos ... Ministry of Science Belgrade University ICGEB more links ... "); array_files[87]=new Array(0,1,"http://www.imgge.bg.ac.yu/photogallery.htm","2009-10-14","2K","IMGGE :: Photogallery ","",""," IMGGE :: Photogallery Back to Home page SimpleViewer requires JavaScript and the Flash Player. Get Flash. "); array_files[88]=new Array(0,1,"http://www.imgge.bg.ac.yu/organisation.htm","2009-10-14","13K","IMGGE :: Organisation ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGE :: Organisation Home IMGGE (formerly Center for Genetic Engineering) was founded in 1986 ... Director, Chairman of the Board ... You can reach us by bus: 33, 39 or 25 ... See our history, events or take virtual tour photos ... Ministry of Science Belgrade University ICGEB more links ... "); array_files[89]=new Array(0,1,"http://www.imgge.bg.ac.yu/laboratories.htm","2009-10-14","14K","IMGGE :: Laboratories ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGE :: Laboratories Home Organisational Chart Director, Chairman of the Board ... You can reach us by bus: 33, 39 or 25 ... See our history, events or take virtual tour photos ... Ministry of Science Belgrade University ICGEB more links ... "); array_files[90]=new Array(0,4,"http://www.imgge.bg.ac.yu/Files/IMGGE_ISO.pdf","2009-10-14","119K"," IMGGE_ISO.doc ","","","C E R T I F I C A T E DQS GmbH Deutsche Gesellschaft zur Zertifizierung von Managementsystemen hereby certifies that the company INSTITUTE OF MOLECULAR GENETICS & GENETIC ENGINEERING Vojvode Stepe 444A, 11000 Belgrade SERBIA for the scope Fundamental research in molecular biology, molecular genetics and biotechnology; Application and improvement of molecular genetics and biotechnology techniques in human and veterinary medicine, agriculture, food production, and pharmaceutical industry; Activities in education has implemented and maintains a Quality Management System. An audit, documented in a report, has verified that this quality management system fulfils the requirements of the following standard: DIN EN ISO 9001 : 2008 November 2008 edition This certificate is valid until Certificate Registration No.: Frankfurt am Main 2012-07-01 434763 QM 2009-07-02 Ass. iur. M. Drechsel MANAGING DIRECTORS Dipl.-Bw. J. Böge Ü»«¬-˝¸»® ßµµ®»Ľ·¬·»®«˛ąÎż¬ ĚŮßóĆÓóđîóçđ D-60433 Frankfurt am Main, August-Schanz-Straße 21 CERTIFICATE IQNet and DQS GmbH Deutsche Gesellschaft zur Zertifizierung von Managementsystemen hereby certify that the company INSTITUTE OF MOLECULAR GENETICS & GENETIC ENGINEERING Vojvode Stepe 444A, 11000 Belgrade SERBIA for the scope Fundamental research in molecular biology, molecular genetics and biotechnology; Application and improvement of molecular genetics and biotechnology techniques in human and veterinary medicine, agriculture, food production, and pharmaceutical industry; Activities in education has implemented and maintains a Quality Management System. An audit, documented in a report, has verified that this quality management system fulfils the requirements of the following standard: ISO 9001 : 2008 This certificate is valid until Frankfurt am Main 2012-07-01 2009-07-02 Registration Number: DE ­ 434763 QM René Wasmer President of IQNet Ass. iur. M. Drechsel J. Böge Managing Directors of DQS GmbH IQNet Partners*: AENOR Spain AFNOR Certification France AIB-Vinçotte International Belgium ANCE Mexico A "); array_files[91]=new Array(0,1,"http://www.imgge.bg.ac.yu/Srb/organisation.htm","2009-10-13","13K","IMGGE :: Organisation ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGE :: Organisation Home IMGGE (formerly Center for Genetic Engineering) was founded in 1986 ... Chairmen, Secretary Finance ... You can reach us by bus: 33, 39 or 25 ... See ours history, events or take virtual tour photos ... Ministry of Science Belgrade University ICGEB more links ... "); array_files[92]=new Array(0,1,"http://www.imgge.bg.ac.yu/Srb/Laboratorije/03/staff.htm","2009-10-13","17K","IMGGI :: Osoblje ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGI :: Osoblje Pocetna > Laboratorije > Laboratorija za Molekularnu Biologiju Rukovodilac laboratorije : Dr. Snežana KOJIĆ, naučni saradnik Professor Emeritus : Professor Dr. Ana SAVIĆ, redovni profesor Saradnici : Dr. Dragica RADOJKOVIĆ, naučni savetnik Mr. Valentina ĐORĐEVIĆ, istaživač-saradnik Dr. Jelena KUŠIĆ, naučni saradnik Marija STANKOVIĆ, istaživač-saradnik Dr. Aleksira DIVAC, naučni saradnik Branko TOMIĆ, istaživač-saradnik Mr. Ljiljana RAKIĆEVIĆ, istraživač-saradnik Mila LJUJIĆ, istraživač-saradnik Mr. Aleksandra NIKOLIĆ, istraživač-saradnik Aleksandra NESTOROVIĆ, istraživač-saradnik Dr. Dragana STEFANOVIĆ, u penziji Završeni i aktivni projekti, istraživački uspesi ... Naši projekti objavljeni u arhivama i časopisima bioloških nauka ... Pogledajte spisak i opis naših komercijalnih usluga ... Ministarstvo Nauke Beogradski Univerzitet ICGEB ostali ... "); array_files[93]=new Array(0,1,"http://www.imgge.bg.ac.yu/Srb/vesti.htm","2009-10-12","14K","IMGGI :: Arhiva vesti ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGI :: Arhiva vesti 28.09-01.10.2009. Ruski naučnici u poseti IMGGI 29.06.2009. Janez Potočnik, evropski komesar za nauku i Božidar Đelić, ministar za nauku i tehnološki razvoj RS  posetili IMGGI 19.06.2009. Prezentacija firme LKB 07.05.2009. Alfatrade: Prezentacija i demonstracija rada QIAcube aparata 29.06.2009. Predstavnici Svetske Banke posetili IMGGI 05-07.12.2008. Festival Nauke, 5th - 7th December 2008 Pogledajte naše slike ... IMGGE (bivši Centar za Genetičko inženjerstvo) je osnovan 1986 godine ... Do nas možete doći autobuskim linijama : 33, 39 ili 25 ... Ministarstvo Nauke Beogradski Univerzitet ICGEB ostali ... "); array_files[94]=new Array(0,1,"http://www.imgge.bg.ac.yu/Srb/scientific.htm","2009-10-12","15K","IMGGI :: Naučno Veće ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGI :: Naučno Veće Pocetna Članovi Naučnog Veća su: •  dr Vesna Maksimović, naučni savetnik, predsednik •  dr Milena Stevanović, naučni savetnik •  dr Branka Vasiljević, naučni savetnik •  dr Dragica Radojković, naučni savetnik, zamenik predsednika •  dr Miroslav Konstantinović, naučni savetnik, zamenik predsednika •  dr Milan Kojić, naučni savetnik •  dr Gordana Nikčević, viši naučni saradnik •  dr Jovanka Miljuš Đukić, viši naučni saradnik •  dr Ivana Strahinić, viši naučni saradnik •  dr Sonja Pavlović, viši naučni saradnik •  dr Nataša Golić, viši naučni saradnik •  dr Amarela Terzić Vidojević, naučni saradnik •  dr Snežana Kojić, naučni saradnik •  dr Jelena Kušić, naučni saradnik •  dr Ivana Morić, naučni saradnik Izbori u zvanja ... Upravni odbor, Direktor, Zamenik direktora, Naučno veće, Laboratorije ... Predsednik Upravnog odbora, Sekretar za finansije ... Do nas možete doći autobuskim linijama : 33, 39 ili 25 ... Pogledajte naše slike ... Ministarstvo Nauke Beogradski Univerzitet ICGEB ostali ... "); array_files[95]=new Array(0,1,"http://www.imgge.bg.ac.yu/Srb/skoladnk.htm","2009-10-12","14K","IMGGE : Mala skola DNKlogije ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGE : Mala skola DNKlogije Pocetna Poštovane kolege, Ministarstvo za nauku i tehnološki razvoj Republike Srbije podržava popularizaciju i promociju nauke i kroz finansiranje projekata iz ove oblasti. Jedan od tih projekata je i  „Mala škola DNKlogije“. Ovaj projekat realizuje tim naučnika, molekularnih biologa, iz Instituta za molekularnu genetiku i genetičko inženjerstvo (IMGGI) iz Beograda. „Mala škola DNKlogije“  doprinosi promociji i popularizaciji molekularne biologije kroz „uradi sam“ eksperimente u laboratoriji. Program ovog Projekta se realizuje kroz dva vida aktivnosti: Putujuća laboratorija i Radionica. Putujuća laboratorija je namenjena predškolskim ustanovama i osnovnim školama. Realizatori projekta dolaze u Vašu školu, i učionicu pretvaraju u laboratoriju. U toku jednog školskog časa učenici sami rade eksperiment: „DNK, to sam ja“  (posmatranje ćelija bukalne sluzokože svakog polaznika pod mikroskopom i izolacija DNK iz  njih)  i  usvajaju znanja iz molekularne biologije. U svim eksperimentima se koriste hemikalije koje se svakodnevno upotrebljavaju (tečni sapun, sok od ananasa, kuhinjska so itd), te su eksperimenti bezbedni u potpunosti za učenike. Radionica je namenjena učenicima srednjih škola, studentima i profesorima biologije u osnovnim i srednjim školama, kao i opštoj populaciji. Radionica se organizuje u prostorijama IMGGI i obuhvata „uradi sam“ eksperimente: „DNK za poneti“ (izolovanje molekula DNK iz biljnih ili humanih ćelija) i „Ko je dirao moje krpice“  (DNK fingerprint). U prethodnom periodu naš program je bio veoma uspešan i omiljen među nastavnicima i učenicima. Za sve informacije i rezervaciju termina možete se obratiti Violeti Evtov, Institut za molekularnu genetiku i genetičko inženjerstvo, Vojvode Stepe 444a, 11010 Beograd. Telefon: 3976445; e-mail: dnklogija@imgge.bg.ac.rs; www.imgge.bg.ac.rs Board, Director, Deputy Director, Scientific council, Laboratories ... Chairmen, Secretary Finance ... You can reach us by bus: 33, 39 or 25 ... See ours history, events or take virtual "); array_files[96]=new Array(0,1,"http://www.imgge.bg.ac.yu/Srb/iso.htm","2009-10-11","15K","IMGGE : Politika kvaliteta ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGE : Politika kvaliteta Pocetna Institut za molekularnu genetiku i genetičko inženjerstvo (IMGGI) teži da bude vodeća institucija u jugoistočnoj Evropi u oblasti molekularne genetike i biotehnologije, kao i vodeća institucija u Srbiji u oblasti: fundamentalnih istraživanja u molekularnoj biologiji, molekularnoj genetici, tehnologiji rekombinantne DNK i biotehnologiji, pri čemu će projekti Instituta biti fokusirani na analizu organizacije genoma i regulacije ekspresije gena različitih organizama; biotehnološke i komercijalne primene i unapređenja tehnika molekularne genetike i biotehnologije u oblasti medicine, veterine, poljoprivrede, prehrambene i farmaceutske industrije; obrazovanja, gde Institut, kao deo Univerziteta u Beogradu, učestvuje u organizovanju i izvođenju programa teorijske i praktične nastave u okviru redovnih, doktorskih i posledoktorskih studija u cilju racionalizacije i poboljšanja kvaliteta nastave na Univerzitetu; obučavanja, u vidu organizovanja kratkih teorijskih i praktičnih (domaćih i međunarodnih kurseva, pojedinačnih ili grupnih) o osnovnim tehnikama molekularne genetike i njihove primene; organizovanja mreže centara molekularne genetike u regionu u cilju daljeg poboljšavanja i intenziviranja aktivnosti u obrazovanju i obučavanju kako u zemlji tako i u regionu, kao i u cilju poboljšanja protoka ljudi u regionu i u zemljama Evropske zajednice. Radi realizacije ovih ciljeva Institut će: Održavati i unapređivati znanja svih zaposlenih permanentnim usavršavanjem kroz odgovarajuću obuku i povećavati njihovu motivaciju za rad i odgovornost za kvalitet poboljšavanjem uslova rada i životnog standarda. Nastojati da stalno zadovolji potrebe i očekivanja svojih korisnika pružanjem brzih i kvalitetnih usluga u skladu sa najvišim svetskim standardima. Kontinuirano poboljšavati sistem kvaliteta Radi realizacije ovih ciljeva Institut će uspostaviti sistem kvaliteta prema standardu SRPS ISO 9001:2008. Za operativno uspostavljanje, održavanje i neprekidno poboljšanje sistema kvaliteta, kao i za tum "); array_files[97]=new Array(0,1,"http://www.imgge.bg.ac.yu/Srb/izbori.htm","2009-10-11","15K","IMGGE : Izbori u zvanja ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGE : Izbori u zvanja Pocetna 17.09.2009. Na šestoj redovnoj  sednici Naučnog veća Instituta za molekularnu genetiku i genetičko inženjerstvo, održane dana 17.09.2009. godine, pokrenut je postupak za izbor dr Amarele Terzić Vidojević u naučno zvanje viši naučni saradnik. Izveštaj Komisije za sprovođenje postupka sa propratnim materijalom može se dobiti na uvid u kadrovskoj službi Instituta, u roku od 30 dana od dana objavljivanja oglasa u dnevnom listu «Politika». 18.06.2009. Na petoj redovnoj  sednici Naučnog veća Instituta za molekularnu genetiku i genetičko inženjerstvo, održanj dana 18.06.2009. godine, pokrenut je postupak za reizbor dr Snežane Kojić u naučno zvanje naučni saradnik. Izveštaj Komisije za sprovođenje postupka sa propratnim materijalom može se dobiti na uvid u kadrovskoj službi Instituta, u roku od 30 dana od dana objavljivanja oglasa u dnevnom listu «Politika». 11.05.2009. Na četvrtoj redovnoj  sednici Naučnog veća Instituta za molekularnu genetiku i genetičko inženjerstvo, održanj dana 11.05.2009. godine, pokrenut je postupak za reizbor dr Milije Jovanović u naučno zvanje naučni saradnik. Izveštaj Komisije za sprovođenje postupka sa propratnim materijalom može se dobiti na uvid u kadrovskoj službi Instituta, u roku od 30 dana od dana objavljivanja oglasa u dnevnom listu «Politika». 02.04.2009. Na trećoj redovnoj  sednici Naučnog veća Instituta za molekularnu genetiku i genetičko inženjerstvo, održanj dana 02.04.2009. godine, pokrenut je postupak za izbor dr Andrijane Klajn u naučno zvanje naučni saradnik. Izveštaj Komisije za sprovođenje postupka sa propratnim materijalom može se dobiti na uvid u kadrovskoj službi Instituta, u roku od 30 dana od dana objavljivanja oglasa u dnevnom listu «Politika». 17.02.2009. Na drugoj redovnoj  sednici Naučnog veća Instituta za molekularnu genetiku i genetičko inženjerstvo, održanj dana 17.02.2009. godine, pokrenut je postupak za izbor dr Marije Mojsin u naučno zvanje naučni saradnik. Izveštaj Komisije za sprovođenje postupka sa propratnim materijalom m "); array_files[98]=new Array(0,4,"http://www.imgge.bg.ac.yu/Srb/Files/iso.pdf","2009-09-15","79K"," IMGGE_serbian_ISO 9001.doc ","","","S E R T I F I K A T DQS GmbH Deutsche Gesellschaft zur Zertifizierung von Managementsystemen ovim potvruje da je organizacija Institut za molekularnu genetiku i geneticko inzenjerstvo Vojvode Stepe 444A, 11000 Beograd SRBIJA za podrucje Naucno-istrazivacka delatnost u oblasti molekularne biologije, molekularne genetike i biotehnologije; Primena i unapreenje tehnika molekularne genetike i biotehnologije u oblasti medicine, veterine, poljoprivrede, prehrambene i farmaceutske industrije; Obrazovna delatnost primenila i odrzava Sistem menadzmenta kvalitetom. Audit, dokumentovan u izvestaju, je pokazao da sistem menadzmenta kvalitetom ispunjava zahteve standarda: DIN EN ISO 9001: 2008 izdanje Novembar 2008 Ovaj sertifikat vazi do Registracioni broj sertifikata: Frankfurt na Majni 2012-07-01 434763 QM 2009-07-02 Ass. iur. M. Drechsel DIREKTORI Dipl.-Bw. J. Böge Ü»«¬-˝¸»® ßµµ®»Ľ·¬·»®«˛ąÎż¬ ĚŮßóĆÓóđîóçđ D-60433 Frankfurt am Main, August-Schanz-Straße 21 "); array_files[99]=new Array(0,1,"http://www.imgge.bg.ac.yu/Srb/lkb.htm","2009-09-15","14K","IMGGE : LKB ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGE : LKB Pocetna U Institutu za molekularnu genetiku i geneticko inženjerstvo (IMGGI) 19. juna 2009. održana je prezentacija firme LKB. Predavanja na engleskom i srpskom jeziku obuhvatala su teorijske i prakticne aspekte i novine u više oblasti medu kojima i opticka masena detekcija u ispitivanjima bioloških interakcija u realnom vremenu, 2-D elektroforeza, DIGE (Difference in Gel Electrophoresis) tehnologija, Real Time PCR. IMGGI, zajedno sa LKB-om, ugostila je brojne goste kako iz inostranstva tako i iz bioloških i medicinskih institucija širom Srbije. Predavaci: 1. Andrew James, GEHC Life Sciences, UK: - In Cell analyzer instrumenti i tehnologija; - MicroCal instrument i tehnologija za mikrokalorimetrijsko odredjivanje stoihiometrije i intermolekularnih odnosa ligan/receptor. 2. Eleni Katsini, HVD Life Sciences GmbH, Vienna, Austria: - Biacore tehnology and special use in food analysis. 3. Nebojsa Dovezenski, LKB Vertriebs GmbH Predstavnistvo Beograd: - Savremeni pristup 2-D elektroforezi, izbor instrumenata, proteomiks; - 2-D DIGE (Difference in Gel Electrophoresis) tehnologija i softveri za analizu. 4. Petio Petkov, LKB Vertriebs GmbH Sofia, BG: Prezentacija EvoCycler HD12 HD-PCR isntrumenta i tehnologije, EvoGEN Corporation. Upravni odbor, Direktor, Zamenik direktora, Naučno veće, Laboratorije ... Predsednik Upravnog odbora, Sekretar za finansije ... Do nas možete doći autobuskim linijama : 33, 39 ili 25 ... Pogledajte naše slike ... Ministarstvo Nauke Beogradski Univerzitet ICGEB ostali ... "); array_files[100]=new Array(0,1,"http://www.imgge.bg.ac.yu/Srb/Laboratorije/03/projects.htm","2009-08-02","15K","IMGGI :: Projekti ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGI :: Projekti Pocetna > Laboratorije > Laboratorija za Molekularnu Biologiju Kodirajuće i nekodirajuće sekvence DNK (MNTRS, 03E12, 1996-2000) Molekulska dijagnostika naslednih oboljenja u medicini (MNTRS, S.6.35.75.0126, 1996-2000) Molecular diagnostics of genetic diseases (ICGEB, CRP/YUG-96-05, 1997-2000) Mutirani gen u kontekstu drugih gena (MNZŽS, 101417, 2002-2004) Creation and use of the library of aptamers highly specific for HsORC4 (ICGEB, CRP/YUG03-01, 2004-2006) “EUROGENTEST” (EU, Network of excellence, FP6-512148, 2005-2009) The role of human skeletal muscle protein Ankrd2/Arpp in striated muscle (ICGEB, CRP/YUG05-01, 2006-2008) Strukturalni elementi genoma u modulaciji fenotipa (MNZŽS, 143051, 2006-2010) Uticaj inicijacionog proteina na strukturu DNK (ICGEB, CRP) Naši projekti objavljeni u arhivama i časopisima bioloških nauka ... U našoj laboratoriji radi 4 Dr, 5 Mr i jedan BSc ... Pogledajte spisak i opis naših komercijalnih usluga ... Ministarstvo Nauke Beogradski Univerzitet ICGEB ostali ... "); array_files[101]=new Array(0,1,"http://www.imgge.bg.ac.yu/Srb/Laboratorije/03/lab.htm","2009-08-02","15K","IMGGI :: Laboratorija za Molekularnu Biologiju ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGI :: Laboratorija za Molekularnu Biologiju Pocetna > Laboratorije Naučnoistraživački interes Laboratorije za molekularnu biologiju uključuje: 1. Korelacije genotipa sa fenotipom Izučavaju se efekti mutacija, zavisno od njihovog tipa i lokacije u genu, za gene za koje smo u našoj populaciji odredili tip i učestalost pojedinih alela. Naša istraživanja su usmerena na CFTR gen i gene koji doprinose nastanku trombofilija i hemofilije (FII, FV, FVIII, FIX, MTHFR), posebno na slučajeve sa istim mutacijama, a različitim fenotipom. 2. Identifikovanje mutacija i polimorfizama u CFTR–opatijama Osim cistične fibroze (CF), najčešćeg teškog oboljenja belaca, neke mutacije u CFTR genu mogu da se dovedu u vezu sa monosimptomatskim oboljenjima, kao što su hronični pankreatitis, CBAVD, diseminirane bronhiektazije (CFTR-opatije). U pacijenata sa navedenim oboljenjima ispituje se korelacija ovih mutacija i polimorfizama sa fenotipom. 3. Primena molekularne biologije u medicini U saradnji sa medicinskim ustanovama, obavlja se prenatalna i postnatalna dijagnostika za CF i hemofiliju A i B, a kod pacijenata sa trombofilijom određuju se mutacije u genima za FII, FV i MTHFR. Sličnim eksperimentalnim pristupom vrši se i testiranje očinstva. 4. Interakcije DNK i proteina; replikacija DNK kod eukariota Izučavanje regulacije inicijacije replikacije DNK, formiranje neortodoksnih struktura DNK, interacija proteina i DNK. 5. Funkcionalna karakterizacija sarkomernih proteina u neuromišićnim bolestima Na primeru sarkomernog proteina Ankrd2 izučava se funkcionalna povezanost proteina Z trake za koje je pokazano da nemaju samo strukturnu već i regulatornu funkciju. Protein Ankrd2 je nedavno identifikovan i delimično okarakterisan, a sledi izučavanje njegove pretpostavljene funkcije glasnika koji prenosi signale od sarkomere do jedra mišićne ćelije gde reguliše ekspresiju ciljnih gena. Izučavaće se i potencijalna uloga proteina Ankrd2 kao i nekih drugih sarkomernih proteina u nastanku neuromišićnih bolesti. U našoj laboratoriji radi 5 Dr, "); array_files[102]=new Array(0,1,"http://www.imgge.bg.ac.yu/Srb/equipment.htm","2009-08-02","17K","IMGGI :: Institutski kapaciteti ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGI :: Institutski kapaciteti Pocetna Sekvenciranje i analiza fragmenata DNK Za ovu svrhu u institutu se koristi Applied Biosystems 3130 Genetic Analyzer, četvorokapilarni automatizovani sistem za elektroforezu sa sistemom za detekciju zasnovanim na fluorescenciji. Automatski sekvencer se takođe može koristiti i za brojne druge aplikacije zasnovane na analizi fragmenata DNK (LOH, MLPA, DGGE, SSCP, MCA, AFLP, SNPlex, SNaPshot). Podaci dobijeni sekvenciranjem obrađuju se pomoću softvera Sequencing Analysis, SeqScape i MicroSeq ID Analysis, dok se pomoću softvera GeneMapper obrađuju podaci dobijeni analizom fragmenata. Real-time PCR sistem Kvantifikacija iRNK (apsolutnom ili relativnom metodom kvantifikacije genske ekspresije), detekcija polimorfizama (SNP), kao i detekcija prisustva ili odsustva specifične ciljne sekvence (Plus/minus eseji) omogućena je “Real–time” PCR Sistemom 7500 firme Applied Biosystems. Bioanalyzer 2100 Zasnovan na Lab-on-a-Chip tehnologiji mikrofluida, koristi se za analizu ćelija, nukleinskih kiselina i proteina. U mikročipovima se kapilarnom elektroforezom razdvajaju male količine uzoraka i već za 30min se dobijaju rezultati analize u digitalnoj formi i visokog kvaliteta. Sistemi za separaciju/razdvajanje makromolekula Osim klasičnih laboratorijskih Sorvall centrifuga (Du Pont Instruments), mikrocentrifuga (Eppendorf) sa i bez hladjenja, institut poseduje i nekoliko ultracentrifuga od kojih izdvajamo Optima L-80 XP. To je nova, poboljšana preparativna ultracentrifuga, koja može da generiše 602 000 g pri brzinama do 80 000 rpm. Njen Optima eXPert Software omogućava optimizaciju uslova centrifugiranja. Zahvaljujući sistemima za hladjenje koji ne štete okolini eliminiše se potreba za CFC jedinjenjima. Sistemi za vizualizaciju DNK i proteina Za dokumentaciju i analizu agaroznih i poliakrilamidnih gelova koristimo sistem BioDocAnalyse firme Biometra. Pomoću Cyclone Storage Phosphor System (Perkin Elmer) se izvode autoradiografije elektroforetskih gelova, blotova, uzoraka tankoslojne hromato "); array_files[103]=new Array(0,1,"http://www.imgge.bg.ac.yu/Srb/copyright.htm","2009-08-02","14K","IMGGE : About ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGE : About Institute of Molecular Genetics i Genetic Engineering (IMGGE - formerly Centre for Genetic Engineering) was founded in 1986. The Institute is associated to International Center for Genetic Engineering i Biotechnology (ICGEB), Trieste, Italy, as an affiliated centre i it is involved in all activities within this international organization. In the field of fundamental research, Institutes projects are mostly focused on research related to analysis of genome organisation i regulation of gene expression in different organisms. Furthermore, IMGGE is also oriented to biotechnological i commercial applications in human i veterinary medicine, agriculture, food production, i pharmaceutical industry. Foundation “Goran Ljubijankić” Representatives of the World Bank visited IMGGI Science Festival, 5th - 7th December 2008 ICGEB funding programmes Pogledajte naše slike ... IMGGE (bivši Centar za Genetičko inženjerstvo) je osnovan 1986 godine ... Do nas možete doći autobuskim linijama : 33, 39 ili 25 ... Ministarstvo Nauke Beogradski Univerzitet ICGEB ostali ... "); array_files[104]=new Array(0,1,"http://www.imgge.bg.ac.yu/Srb/board.htm","2009-08-02","13K","IMGGI :: Upravni odbor ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGI :: Upravni odbor Pocetna Prof. Pavle MILENKOVIĆ, Dr Stomatološki fakultet u Beogradu v.d. predsednika Upravnog odbora Dr Miodrag PETROVIĆ IBISS, v.d. člana Dr Snežana PAJOVIĆ INN «Vinča», v.d. člana Prof.dr Slavica SPASIĆ Farmaceutski fakultet u Beogradu, v.d. člana Dr Milena STEVANOVIĆ IMGGI, v.d. člana Dr Gordana NIKČEVIĆ IMGGI zamenik v.d. predsednika Dr Ivana STRAHINIĆ IMGGI, v.d. člana Dr Vesna MAKSIMOVIĆ IMGGI, v.d. člana Rešenje Vlade RS 24br.119-1295/2008 od 27.marta 2008.godine Upravni odbor, Direktor, Zamenik direktora, Naučno veće, Laboratorije ... Predsednik Upravnog odbora, Sekretar za finansije ... Do nas možete doći autobuskim linijama : 33, 39 ili 25 ... Pogledajte naše slike ... Ministarstvo Nauke Beogradski Univerzitet ICGEB ostali ... "); array_files[105]=new Array(0,1,"http://www.imgge.bg.ac.yu/Srb/administration.htm","2009-08-02","14K","IMGGI :: Administracija ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGI :: Administracija Pocetna DIREKTOR: Branka VASILJEVIĆ, Dr Tel: (+381 11) 3975 744 E-mail: vasiljb@eunet.rs Predsednik UPRAVNOG ODBORA : Pavle MILENKOVIĆ, Dr Tel: (+381 11) 2684 484 E-mail: mpavle@imi.bg.ac.rs Predsednik NAUČNOG VEĆA : Vesna MAKSIMOVIĆ, Dr Tel: (+381 11) 3976 414 E-mail: heljda@sezampro.rs SEKRETAR: Violeta EVTOV Tel: (+381 11) 3975 744 E-mail: beogene@eunet.rs FINANSIJE: Duško VUJIĆ Maja JEVTIĆ Tel: (+381 11) 3976 065 E-mail: imggi@sezampro.rs FAX : (+381 11) 3975 808 IMGGE (bivši Centar za Genetičko inženjerstvo) je osnovan 1986 godine ... Upravni odbor, Direktor, Zamenik direktora, Naučno veće, Laboratorije ... You can reach us by bus: 33, 39 or 25 ... Pogledajte naše slike ... Ministry of Science Belgrade University ICGEB more links ... "); array_files[106]=new Array(0,1,"http://www.imgge.bg.ac.yu/Srb/usefullinks.htm","2009-08-02","14K","IMGGI :: Korisni linkovi ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGI :: Korisni linkovi Pocetna Ministarstvo za nauku i tehnološki razvoj Univerzitet u Beogradu Biološki fakultet Narodna biblioteka Srbije Univerzitetska biblioteka Svetozar Markovic International Center for Genetic Engineering i Biotechnology - ICGEB EUROGENTEST CF Network NIZO European Plant Science Organization The Serbian Mutation Frequency database ORPHANET Upravni odbor, Direktor, Zamenik direktora, Naučno veće, Laboratorije ... Predsednik Upravnog odbora, Sekretar za finansije ... Do nas možete doći autobuskim linijama : 33, 39 ili 25 ... Pogledajte naše slike ... "); array_files[107]=new Array(0,1,"http://www.imgge.bg.ac.yu/Srb/shorthistory.htm","2009-08-02","16K","IMGGI :: Istorijat ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGI :: Istorijat Pocetna Institut za Molekularnu Genetiku i Genetičko Inženjerstvo (IMGGI – nekadašnji Centar za genetičko inženjerstvo) osnovan je 1986. godine, pošto je Jugoslovenski parlament usvojio Zakon o ratifikaciji  statuta Internacionalnog Centra za Genetičko Inženjerstvo i Biotehnologiju (ICGEB). Na ovaj način Institut je povezan sa ICGEB-om u Trstu, Itlija, kao njegov pridruženi centar i uključen je u sve aktivnosti ove meunarodne organizacije.Prvi direktor, koji je ujedno i utemeljivač IMGGI-a bio je Profesor Vladimir Glišin. IMGGI ima dobru saradnju sa mnogim institutima u regionu kao i sa zemljama članicama ICGEB-a. Od 1998. godine IMGGI se nalazi u novoj zgradi na površini od 2400 m2 i raspolaže kompletnom opremom koja je neophodna za sofisticirane eksperimentalne manipulacije genetičkog inženjerstva. Odlukom Vlade Republike Srbije 05 No. 022-1183/2007 od 1.03.2007. godine  IMMGI postaje državni institut. Većina aktivnosti Instituta usmerena je ka fundamentalnim istraživanjima u molekularnoj biologiji, molekularnoj genetici, tehnologiji rekombinantne DNK i biotehnologiji. U oblasti fundamentalnih istraživanja, projekti Instituta su najvećim delom fokusirani na istraživanja vezana za analizu genomske organizacije i regulacije genske ekspresije kod raznih organizama. Pored toga, jedan deo svojih aktivnosti IMGGI  realizuje kroz biotehnološke  i komercijalne aspekte u humanoj i veterinarskoj medicini, poljoprivredi, proizvodnji hrane i farmaceutskoj industriji. Institut čini šest laboratorija: Laboratorija za molekularnu genetiku industrijskih mikroorganizama, Laboratorija za molekularnu genetiku aktinomiceta, Laboratorija za molekularnu biologiju biljaka, Laboratorija za humanu molekularnu genetiku, Laboratorija za molekularnu biologiju i Laboratorija za molekularnu hematologiju. U Institutu je trenutno zaposleno 65 istraživača. Pored stalno zaposlenih, Institut je često domaćin istrazivačima iz drugih istraživačkih institucija. Institut je članica Univerziteta u Beogradu. Kroz Institut nepreki "); array_files[108]=new Array(0,1,"http://www.imgge.bg.ac.yu/Srb/reachus.htm","2009-08-02","12K","IMGGI :: Lokacija ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGI :: Lokacija Pocetna Do nas možete doći autobuskim linijama : 33, 39 ili 25 GSP Beograd PlanPlus Takođe, možete nas posetiti kolima.Pogledajte priloženu mapu.. Upravni odbor, Direktor, Zamenik direktora, Naučno veće, Laboratorije ... Predsednik Upravnog odbora, Sekretar za finansije ... Pogledajte naše slike ... Ministarstvo Nauke Beogradski Univerzitet ICGEB ostali ... "); array_files[109]=new Array(0,1,"http://www.imgge.bg.ac.yu/Srb/photogallery.htm","2009-08-02","2K","IMGGI :: Fotogalerija ","",""," IMGGI :: Fotogalerija Povratak SimpleViewer requires JavaScript i the Flash Player. Get Flash. "); array_files[110]=new Array(0,1,"http://www.imgge.bg.ac.yu/Srb/laboratories.htm","2009-08-02","14K","IMGGI :: Laboratorije ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGI :: Laboratorije Pocetna Upravni odbor, Direktor, Zamenik direktora, Naučno veće, Laboratorije ... Predsednik Upravnog odbora, Sekretar za finansije ... Do nas možete doći autobuskim linijama : 33, 39 ili 25 ... Pogledajte naše slike ... Ministarstvo Nauke Beogradski Univerzitet ICGEB ostali ... "); array_files[111]=new Array(0,1,"http://www.imgge.bg.ac.yu/Srb/fondation.htm","2009-08-02","13K","IMGGI :: Fondacija ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGI :: Fondacija Pocetna Posle tragične smrti Gorana Ljubijankića njegovi roditelji, Danica i Rasim, pokrenuli su inicijativu i finansijski pomogli osnivanje Fondacije “Goran Ljubijankić”. Fondaciju je 1. jula 2003. g. osnovao Institut za molekularnu genetiku i genetičko inženjerstvo, u kojem je Goran proveo najplodnije godine svog naučno-istraživačkog rada. Osnivanje Fondacije, pored Goranovih roditelja i Instituta, pomogle su i kolege iz International Centre for Genetic Engineering and Biotechnology, Trst, Italija. Cilj Fondacije je da pomogne mladim i perspektivnim naučnicima iz naše zemlje koji se bave istraživanjima iz oblasti molekularne biologije. Fondacija «Goran Ljubijankić» će svakog 30. januara, na Goranov rođendan, dodeljivati nagrade najuspešnijim mladim istraživačima za postignute rezultate iz oblasti molekularne biologije.   Upravni odbor, Direktor, Zamenik direktora, Naučno veće, Laboratorije ... Predsednik Upravnog odbora, Sekretar za finansije ... Do nas možete doći autobuskim linijama : 33, 39 ili 25 ... Pogledajte naše slike ... Ministarstvo Nauke Beogradski Univerzitet ICGEB ostali ... "); array_files[112]=new Array(0,1,"http://www.imgge.bg.ac.yu/Srb/festival.htm","2009-08-02","14K","IMGGE : Festival nauke ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGE : Festival nauke Pocetna U okviru štanda «Tajne koda DNK» na Festivalu nauke održanom od 5. do 7. decembra 2008. godine IMGGI je organizovao nagradnu igru «Dešifrujte kod DNK». Zadatak da prevedu redosled nukleotida u DNK u redosled aminokiselina u proteinu rešavalo je 1550 posetilaca festivala. Izvučeno je 10 srećnih dobitnika, koji su 22. januara 2009. godine bili u poseti Institutu i družili se sa istraživačima, a pomalo se i sami bavili naukom. Gosti su imali prilike da iz ćelija sluzokože usta izoluju sopstvenu DNK, analiziraju je i potom vide na gelu od agaroze, kao i da je ponesu sa sobom za uspomenu. Takođe su imali prilike da čuju i vide čime se bave istraživači u IMGGI, kako se bakterije boje i gledaju pod mikroskopom, kako se DNK može obeležiti fluorescentnim bojama i potom analizirati, kako se u kulturi gaje biljne i sisarske ćelije (slike ...). Gosti Instituta su bili: Irma Bosna, učenica VI razreda OŠ «Jovan Miodragović» Matija Veljković, učenik II razreda VII beogradske gimnazije Nikola Glavonjić, učenik II razreda VII beogradske gimnazije Dunja Damjanović, učenica VI razreda OŠ «Vlada Aksentijević» Ksenija Dangubić, učenica IV razreda OŠ «Karađorđe» Marko Đorđević, učenik VI razreda OŠ «Aca Aleksić» u Aleksandrovcu Marko Karanović, učenik VI razreda OŠ «Despot Stefan Lazarević» Aleksa Ratarac, učenik VII razreda OŠ «Kralj Aleksandar I» Mihajlo Stošić, učenik VI razreda OŠ «Radojka Lakić» Ana Todorović, učenica V razreda OŠ «Vlada Aksentijević» Upravni odbor, Direktor, Zamenik direktora, Naučno veće, Laboratorije ... Predsednik Upravnog odbora, Sekretar za finansije ... Do nas možete doći autobuskim linijama : 33, 39 ili 25 ... Pogledajte naše slike ... Ministarstvo Nauke Beogradski Univerzitet ICGEB ostali ... "); array_files[113]=new Array(0,1,"http://www.imgge.bg.ac.yu/Srb/Laboratorije/01/staff.htm","2009-08-02","16K","IMGGI :: Osoblje ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGI :: Osoblje Pocetna > Laboratorije > Laboratorija za molekularnu hematologiju Rukovodilac laboratorije : Dr. Sonja PAVLOVIĆ, viši naučni saradnik Saradnici : Dr. Tatjana KOSTIĆ, naučni saradnik Teodora KARAN ĐURAŠEVIĆ, istraživač-saradnik Mr. Nataša TOŠIĆ, istraživač-saradnik Milena RADMILOVIĆ, istraživač-stipendista Branka ZUKIĆ, istraživač-saradnik Vesna SPASOVSKI, istraživač-pripravnik Maja STOJILJKOVIĆ, istraživač-saradnik Mina GOĐEVAC, diplomac Nikola Kotur, diplomac Dr. Jelena ZARIĆ, viši naučni saradnik, Švajcarska Dr Aleksandra TRIFUNOVIĆ, naučni saradnik, Švedska Mr. Jelena JOVANOVIĆ, istraživač-saradnik, Velika Britanija Mr. Jelena UROŠEVIĆ, istraživač-saradnik, Španija Završeni i aktivni projekti, istraživački uspesi ... Naši projekti objavljeni u arhivama i časopisima bioloških nauka ... Pogledajte spisak i opis naših komercijalnih usluga ... Ministarstvo Nauke Beogradski Univerzitet ICGEB ostali ... "); array_files[114]=new Array(0,1,"http://www.imgge.bg.ac.yu/Srb/Laboratorije/01/publications.htm","2009-08-02","16K","IMGGI :: Publikacije ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGI :: Publikacije Pocetna > Laboratories > Laboratorija za molekularnu hematologiju Kollia P, Kalamaras A, Chassanidis C, Samara M, Vamvakopoulos NK, Radmilović M, Pavlović S, Papadakis MN, Patrinos GP. Compound heterozygosity for the Cretan type of non-deletional heriditary persistence of fetal hemoglobin and beta-thalassemia or Hb Sabine confirms the functional role of the A gamma -158 CT mutation in gamma-globin gene transcription. Blood Cells Mol Dis. 2008 Nov-Dec; 41(3):263-4. Protić MB, Pavlović ST, Bojić DZ, Krstić MN, Radojčić ZA, Tarabar DK, Stevanović AZ, Karan Đurašević TZ, Gođevac MV, Svorcan PV, Dapčević BD, Jojić NZ. CARD15 gene polymorphisms in Serbian patients with Crohn’s disease: genotype-phenotype analysis. Eur J Gastroenterol Hepatol. 2008 Oct; 20(10):978-84. Jevtović-Stojmenov T, Kocić G, Pavlović D, Macukanović-Golubovac L, Marjanović G, Đorđević V, Tošić N, Pavlović S. Polymorphisms of tumor-necrosis factor.alpha -308 and lymphotoxin-alpha +250: possible modulation of susceptibility to apoptosis in chronic lymphocytic leukemia and non-Hodgkin lymphoma mononuclear cells. Leuk Lymphoma. 2008 Nov; 49(11):2163-9. Stojiljkovic M, Stevanovic A, Djordjevic M, Petrucev B, Tosic N, Karan Djurasevic T, Aveic S, Radmilovic M, Pavlović S. Mutations in the PAH gene: a tool for population genetic study. Arch Biol Sci: 2007: 59 (3): 161-167. Colovic N, Tosic N, Aveic S, Djuric M, Milic N, Bumbasirevic V, Colovic M, Pavlovic S. Importance of early detection and follow-up of FLT3 mutations in patients with acute myeloid leukemia. Annals of Hematology 2007; 86: 741-747. 6.Jursic V, Terzic T, Pavlovic S, Colovic N, Colovic M. Elevated TNF-a and LDH without parathormone disturbance is associated with diffuse osteolytic lesions in leukemic transformation of myelofibrosis. Pathology Research and Practice. 2007. Oct30. Vesna Brankovic-Sreckovic, Vedrana Milic Rasic, Valentina Djordjevic, Milos Kuzmanovic, Sonja Pavlovic. Arterial Ischemic Stroke in a Child with ß-Thalassemia Trait and Methylentetrahyd "); array_files[115]=new Array(0,1,"http://www.imgge.bg.ac.yu/Srb/Laboratorije/01/projects.htm","2009-08-02","19K","IMGGI :: Projekti ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGI :: Projekti Pocetna > Laboratorije > Laboratorija za molekularnu hematologiju Strukturalni elementi genoma u modulaciji fenotipa (MNZŽS, 2005-2010) Molekularno genetski markeri klonskog preobražaja matičnih ćelija hematopoeze (MNZŽS, 2005-2010) Unapređenje biomedicinske nauke i tehnologije u cilju poboljšanja zdravlja u Srbiji “HISERBS” (EU, SSA, FP6-INCO-026357, 2006-2009) Naučno istraživački interesi : Molekularna genetika talasemijskih sindroma Talasemijski sindromi su nasledni poremećaji koji su posledica odsustva ili smanjene sinteze jednog ili više polipeptidnih lanaca hemoglobina, što uzrokuje pojavu anemije. Talasemije su posledica velikog broja različitih genetičkih defekata. Molekulska dijagnostika talasemija je najpouzdaniji metod za rano otkrivanje bolesti što, zbog pravovremene primene terapije, popravlja kliničku sliku i tok bolesti, kao i za prenatalnu dijagnozu. Ovaj projekat obuhvata analize hemoglobina  i analize DNK globinskih gena (PCR, reverzni dot blot) u porodicama sa visokim rizikom za talasemiju i identifikaciju novih mutacija u  Srbiji. Mi izučavamo i regulaciju transkripcije fetalnih beta-globinskih gena. Analiziraju se mutacije u Gy I Ay promotoru kao potencijalni modulatori beta-talasemijskog fenotipa. Fenilketonurija: Molekularna karakterizacija i genotip-fenotip korelacija Fenilketonurija (PKU, MIM#261600) je najčešća nasledna bolest vezana za poremećaj metabolizma esencijalne amino kiseline (u beloj populaciji oboleva 1/10000). Uzrok fenilketonurije je poremećaj fenilalanin hidroksilaze (PAH, EC 1.14.16.1), enzima koji u jetri katalizuje prevođenje fenilalanina u tirozin. Posledica poremećenog metaboličkog puta je povišen nivo fenilalanina u krvi i nedostatak tirozina neophodnog za sintezu neurotransmitera. Bolest može izazvati mentalnu retardaciju, ukoliko se ne primeni dijetalni režim ishrane. Ovaj projekat se bavi molekularnom karakterizacijom mutacija koje se javljaju u genu za fenilalanin hidroksilazu i uzrok su fenilketonurije kod pacijenata iz Srbije i Crne Go "); array_files[116]=new Array(0,1,"http://www.imgge.bg.ac.yu/Srb/Laboratorije/01/lab.htm","2009-08-02","13K","IMGGI :: Laboratorija za molekularnu hematologiju ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGI :: Laboratorija za molekularnu hematologiju Pocetna > Laboratorije Naučno istraživački interesi Laboratorije za molekularnu hematologiju su: molekularnagenetička osnova leukemija, talasemijskih sindroma, inflamatornih bolesti creva i fenilketonurija, kao i farmakogenetika i dijagnostika i  praćenje molekularnogenetičkih markera u toku lečenja leukemija. U našoj laboratoriji radi 4 Dr, 3 Mr i 5 BSc ... Završeni i aktivni projekti, istraživački uspesi ... Naši projekti objavljeni u arhivama i časopisima bioloških nauka ... Pogledajte spisak i opis naših komercijalnih usluga ... Ministarstvo Nauke Beogradski Univerzitet ICGEB ostali ... "); array_files[117]=new Array(0,1,"http://www.imgge.bg.ac.yu/Srb/Laboratorije/03/publications.htm","2009-08-02","16K","IMGGI :: Publications ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGI :: Publications Pocetna > Laboratorije > Laboratorija za Molekularnu Biologiju M. Stankovic, A. Nikolic, A. Divac, A. Tomovic, N. Petrovic-Stanojevic, M. Andjelic, V. Dopudja-Pantic, M. Surlan, I. Vujicic, D. Ponomarev, M. Mitic-Milikic, J. Kusic,D. Radojkovic. The CFTR M470V gene variant as a potential modifier of COPD severity – study of Serbian population. Genet Test. 2008;12(3):357 Stefanovic D, Kusic J, Divac A, Tomic B. Formation of noncanonical DNA structures mediated by human ORC4, a protein compjedannt of the origin recognition complex Biochemistry. 2008 Aug 19;47(33):8760-7. Zaimidou S, van Baal S, Smith TD, Mitropoulos K, Ljujic M, Radojkovic D, Cotton RG, Patrinos GP. (2008) A(1)ATVar: a relational database of human SERPINA1 gene variants leading to alpha(1)-antitrypsin deficiency i application of the VariVis software. Hum Mutat. 2008 Nov 19. [Epub ahead of print] Radojkovic M, Ristic S, Colovic M, Cemerikic-Martinovic V, Radojkovic D, Krtolica K. Molecular characteristics i prognostic significance of Bcl-2/IgH gene rearrangement in Serbian follicular lymphoma patients. Neoplasma. 2008; 55(5):421-7. Castellani C, Cuppens H, Macek M Jr, Cassiman JJ, Kerem E, Durie P, Tullis E, Assael BM, Bombieri C, Brown A, Casals T, Claustres M, Cutting GR, Dequeker E, Dodge J, Doull I, Farrell P, Ferec C, Girodon E, Johannesson M, Kerem B, Knowles M, Munck A, Pignatti PF, Radojkovic D, Rizzotti P, Schwarz M, Stuhrmann M, Tzetis M, Zielenski J, Elborn JS. Consensus on the use i interpretation of cystic fibrosis mutation analysis in clinical     practice. J Cyst Fibros. 2008 May;7(3):179-96. Review. Nisevic I, Dinic J, Nikolic A, Djordjevic V, Lukic S, Ugljesic M, Andjelic-Jelic M, Petrovic-Stanojevic N, Radojkovic D. MTHFR C677T polymorphism in chronic pancreatitis i pancreatic adenocarcinoma. Cell Biochem Funct. 2008;26(6):659-63. Ljujic M, Topic A, Divac A, Nikolic A, Petrovic-Stanojevic N, Surlan M, Mitic-Milikic M, Radojkovic D. Isoelectric focusing phenotyping i denaturing gradient gel electrophor "); array_files[118]=new Array(0,1,"http://www.imgge.bg.ac.yu/Srb/Laboratorije/04/lab.htm","2009-08-02","13K","IMGGI :: Laboratorija za Humanu Molekularnu Genetiku ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGI :: Laboratorija za Humanu Molekularnu Genetiku Pocetna > Laboratorije Istraživački interesi Laboratorije za Humanu Molekularnu Genetiku uključuju proučavanje kloniranja i karakterizacije humanih SOX gena i fluorescentne in situ hibridizacije (FISH) kao i njene primena u prenatalnoj dijagnostici, detektovanju naslednih bolesti i u malignoj transformaciji U našoj laboratoriji radi 4 Dr, 5 Mr i jedan BSc ... Završeni i aktivni projekti, istraživački uspesi ... Naši projekti objavljeni u arhivama i časopisima bioloških nauka ... Pogledajte spisak i opis naših komercijalnih usluga ... Ministarstvo Nauke Beogradski Univerzitet ICGEB ostali ... "); array_files[119]=new Array(0,1,"http://www.imgge.bg.ac.yu/Srb/Laboratorije/04/staff.htm","2009-08-02","15K","IMGGI :: Osoblje ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGI :: Osoblje Pocetna > Laboratorije > Laboratorija za Humanu Molekularnu Genetiku Rukovodilac laboratorije : Dr. Milena STEVANOVIĆ Saradnici : Dr. Gordana NIKČEVIĆ Mr. Jelena POPOVIĆ Dr. Marija MOJSIN Mr. Danijela DRAKULIĆ Dr. Andrijana KLAJN Mr. Isidora PETROVIĆ Mr. Nataša KOVAČEVIĆ GRUJIČIĆ Mr. Aleksndar KRSTIĆ Milena MILIVOJEVIĆ, istraživač-saradnik Završeni i aktivni projekti, istraživački uspesi ... Naši projekti objavljeni u arhivama i časopisima bioloških nauka ... Pogledajte spisak i opis naših komercijalnih usluga ... Ministarstvo Nauke Beogradski Univerzitet ICGEB ostali ... "); array_files[120]=new Array(0,1,"http://www.imgge.bg.ac.yu/Srb/Laboratorije/04/publications.htm","2009-08-02","15K","IMGGI :: Publikacije ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGI :: Publikacije Pocetna > Laboratorije > Laboratorija za Humanu Molekularnu Genetiku Petrović, I., Kovačević-Grujičić, N. i Stevanović, M. ZBP-89 i Sp3 down-regulate while NF-Y up-regulates SOX18 promoter activity in HeLa cells. 2008. Molecular Biology Reports, DOI 10.1007/s11033-008-9272-x. Kovačević Grujičić, N., Yokoyama, K. K. i Stevanović, M. Trans-activation of the human SOX3 promoter by MAZ in NT2/D1 cells. 2008. Archives of Biological Sciences, Belgrade, 60, 379-387. Nikčević, G., Savić, T., Kovačević-Grujičić, N. i Stevanović, M. Up-regulation of the SOX3 gene expression by retinoic acid: characterization of the novel promoter-response element i the retinoid receptors involved. 2008. Journal of Neurochemistry, 107, 1206-1215. Krstić, A., Mojsin, M., Kovačević-Grujičić, N. i Stevanović, M. PCR amplification i sequence analysis of the rat Sox3 gene. 2008. Archives of Biological Sciences, Belgrade, 60, 525-530. Djordjević, V., Dencić-Fekete, M., Jovanović, J., Drakulić, D., Stevanović, M., Janković, G. i Gotić, M. Pattern of trisomy 1q in hematological malignancies: a single institution experience. 2008. Cancer Genet Cytogenet. 186, 12-18. Cuturilo, G., Drakulic, D., Stevanovic, M., Jovanovic, I., Djukic, M., Miletic-Grkovic, S. i  Atanaskovic-Markovic, M. A rare association of interrupted aortic arch type C i microdeletion 22q11.2. 2008. Eur J Pediatr. 167, 1195-1198. Krstić, A., Mojsin, M. i Stevanović, M. Regulation of SOX3 gene expression is driven by multiple NF-Y binding elements. 2007. Archives of Biochemistry i Biophysics, 467, 163-173. Kovačević Grujičić, N., Mojsin, M., Djurović, J., Petrović, I. i Stevanović, M. Comparison of promoter regions of SOX3, SOX14 i SOX18 orthologs in mammals. 2008. DNA Sequence, 19, 185-194. Drakulić D., Nikčević, G., Djordjević, V. i Stevanović M. Generation of a whole chromosome painting probe from monochromosomal hybrid cells by the Alu -polymerase chain reaction.2007. Archives of Biological Sciences, 59, 89-95. Nikčević, G., Kovačević Grujičić, N., St "); array_files[121]=new Array(0,1,"http://www.imgge.bg.ac.yu/Srb/Laboratorije/04/projects.htm","2009-08-02","16K","IMGGI :: Projekti ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGI :: Projekti Pocetna > Laboratorije > Laboratorija za Humanu Molekularnu Genetiku Projekti: Kloniranje i karakterizacija humanih SOX gena (MNTRS, 03E11, 1996-2000). Retinoic acid induction of the SOX3 gene (The Wellcome Trust, London UK,037356/Z/92, 1996-2000). FISH (fluorescentna in situ hibridizacija) i njena primena u prenatalnoj dijagnostici, detektovanju naslednih bolesti i u malignoj transformaciji (MNTRS, I.3. 1429, 1996-1997). Razvoj i primena novih biotehnoloških metoda i postupaka za povećanje proizvodnje i poboljšanje kvaliteta mesa, mleka i jaja (MNTRS, S.4.27.48.0001, 1998-2000). Retinoic acid induction of the SOX3 gene expresion (ICGEB, CRP/YUG99-01, 2000-2002). Humani SOX geni: proučavanje strukture i regulacije ekspresije (MNZŽS, 101438 2002-2004). Izučavanje regulacije ekspresije i funkcije humanih SOX gena (MNZŽS,143028, 2006-2010). Regulation of human SOX18 gene expression and its role in angiogenesis (funded by ICGEB, CRP/YUG07-01, 2008-2010). Naučnoistraživački interes: Izučavanje regulacije ekspresije i funkcije humanih SOX gena Članovi familije SOX gena učestvuju u procesima regulacije diferencijacije i razvića kod čoveka. Pretraživanjem cDNK biblioteke mozga fetusa čoveka, klonirani su i okarakterisani humani SOX2, SOX3, SOX14, SOX18 i SOX20 geni. Ciljevi istraživanja: Proučavanje strukture i transkripcione regulacije ekspresije humanih SOX3, SOX14 i SOX18 gena Definisanje kontrolnih elemenata i identifikacija transkripcionih faktora uključenih u regulaciju ekspresije ovihgena Proučavanje aktivacije ekspresije SOX3 gena retinoičnom kiselinom u NT2/D1 ćelijskoj liniji. Proučavanje ekspresije SOX gena u tumorima neuralnog porekla primenom in situ hibridizacije. Izučavanje kontrolnih mehanizama uključenih u transkripcionu regulaciju ekspresije doprineće razumevanju funkcije SOX gena u kontroli procesa diferencijacije i razvića. Ova istraživanja mogu doprineti razumevanju molekularnih mehanizama koji dovode do poremećaja u razviću nervnog sistema čoveka i time omogućiti razvoj di "); array_files[122]=new Array(0,1,"http://www.imgge.bg.ac.yu/Srb/Laboratorije/05/staff.htm","2009-08-02","15K","IMGGI :: Osoblje ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGI :: Osoblje Pocetna > Laboratorije > Laboratorija za Molekularnu Genetiku Aktinomiceta Rukovodilac laboratorije : Dr. Branka VASILJEVIĆ, naučni savetnik Saradnici : Dr. Ivana MORIĆ, naučni saradnik Mr. Miloje SAVIĆ, istraživač saradnik Mr. Sandra VOJNOVIĆ, istraživač saradnik Lidija ĐOKIĆ, istraživač pripravnik Mr. Tatjana ILIĆ TOMIĆ, istraživač saradnik Tanja NARANČIĆ, istraživač pripravnik Mr. Nada STANKOVIĆ, istraživač saradnik Sanja BAJKIĆ, istraživač pripravnik Završeni i aktivni projekti, istraživački uspesi ... Naši projekti objavljeni u arhivama i časopisima bioloških nauka ... Pogledajte spisak i opis naših komercijalnih usluga ... Ministarstvo Nauke Beogradski Univerzitet ICGEB ostali ... "); array_files[123]=new Array(0,1,"http://www.imgge.bg.ac.yu/Srb/Laboratorije/05/publications.htm","2009-08-02","15K","IMGGI :: Publikacije ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGI :: Publikacije Pocetna > Laboratorije > Laboratorija za Molekularnu Genetiku Aktinomiceta Ilić – Tomić, T., Morić, I., Conn, G. L., Vasiljević, B.: Aminoglycoside resistance genes sgm i kgmB protect bacterial but not yeast small ribosomal subunits in vitro despite high conservation of the rRNA site. Res. in Microbiol. 159 (2008) 658 – 662. Savić, M., Ilić – Tomić, T., Macmaster, R., Vasiljević, B., Conn, G. L.: Critical Residues for Cofactor Binding i Catalytic Activity in the Aminoglycoside Resistance Methyltransferase Sgm. J. Bacteriol. 190 (2008) 5855 – 5861. Kojić, M., Vojnović, S., Vukov, N., Vasiljević, B.: Overexpression of Sgm 5’ UTR mRNA reduces gentamicin resistance in both Escherichia coli i Micromonospora melanosporea cells. Arch. Biol.Sci. 59 (2007) 273 – 280. Savić, M., Bratić I., Vasiljević, B.: Streptomyces durmitorensis sp. nov., a producer of an FK506-like immunosuppressant. International Journal of Systematic i Evolutionary Microbiology 57 (2007) 2119-2124. Savić, M., Vasiljević, B.: Targeting polyketide synthase gene pool within actinomycetes: new degenerate primers. J. Ind. Microbiol. Biotechnol. 33 (2006) 423-430. Marković, S., Vojnović, S., Jovanović, M., Vasiljević, B.: Different expression level of two KgmB-His fusion proteins. Journal of the Serbian Chemical Society 70 (2005) 1401-1407. Ilić Tomić, T., Marković, S., Vasiljević, B.: Expression i purification of the Sgm protein from E. coli. Journal of the Serbian Chemical Society 70 (2005) 817-822. Skoko, N., Vujović, J., Savić, M., Papić, N., Vasiljević, B., Ljubijankić, G.: Construction of Saccharomyces cerevisiae strain FAV20 useful in detection of immunosuppressants produced by soil actinomycetes. Journal of Microbiological Methods 61 (2005) 137-140. Vajić, S., Anastasov, N., Vasiljević, B.: The kgmB gene, encoding ribosomal RNA methylase from Streptomyces tenebrarius, is autogenously regulated. Archives of Microbiology. 182 (2004) 475-481. Kojić, M., Milojević, N., Vasiljević, B.: Gentamicin-resistance determinants con "); array_files[124]=new Array(0,1,"http://www.imgge.bg.ac.yu/Srb/Laboratorije/05/projects.htm","2009-08-02","16K","IMGGI :: Projekti ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGI :: Projekti Pocetna > Laboratorije > Laboratorija za Molekularnu Genetiku Aktinomiceta Projekti: Izučavanje strukture i funkcije gena industrijskih mikroorganizama, Molekularna genetika mikromonospora (MNTRS, 1996-2000). Regulation of gene expression in antibiotic producer Streptomyces tenebrarius (ICGEB/CRP/YUG00-05). Molekularna genetika mikroorganizama i ekspresija gena od ekonomskog značaja (ev. br. 1512, MNTRS, 2002-2005). Ekspresija i regulacija farmaceutski značajnih gena u mikroorganizmima (ev.br.143056, MNZŽS, 2006-2010). Structure-function studies of 16S rRNA methyltransferases: the basis of aminoglycoside antibiotic resistance and enzyme translational autoregulation (Wellcome Trust, 2006-2008). Search for new bioactive secondary metabolites (Bilateralni projekat, saradnja akademija nauka i u metnosti Srbije i Republike Češke, 2006-2007). Molekularna genetika aktinomiceta, bakterija koje proizvode sekundarne metabolite Izučavanje mehanizama rezistencije aktinomiceta na aminoglikozidne antibiotike. Kloniranje gena odgovornih za rezistenciju na aminoglikozidne antibiotike. Izučavanje regulacije ekspresije gena odgovornih za rezistenciju. Interakcija metilaza odgovornih za rezistenciju na aminoglikozidne antibiotike sa 16S rRNK i regulatornim sekvencama na iRNK. Formiranje kolekcije aktinomiceta izolovanih sa različitih lokaliteta, filogenetska analiza. Izučavanje sekundarnih metabolita. Poliketid sintaze (PKS) aktinomiceta i njihova uloga u sintezi imunomodulatornih jedinjenja. Naši projekti objavljeni u arhivama i časopisima bioloških nauka ... U našoj laboratoriji radi 2 Dr, 4 Mr i 3 BSc ... Pogledajte spisak i opis naših komercijalnih usluga ... Ministarstvo Nauke Beogradski Univerzitet ICGEB ostali ... "); array_files[125]=new Array(0,1,"http://www.imgge.bg.ac.yu/Srb/Laboratorije/05/lab.htm","2009-08-02","12K","IMGGI :: Laboratorija za Molekularnu Genetiku Aktinomiceta ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGI :: Laboratorija za Molekularnu Genetiku Aktinomiceta Pocetna > Laboratorije Laboratorija za molekularnu genetiku aktinomiceta se bavi izučavanjem aktinomiceta koje proizvode sekundarne metabolite. U našoj laboratoriji radi 2 Dr, 4 Mr i 3 BSc ... Završeni i aktivni projekti, istraživački uspesi ... Naši projekti objavljeni u arhivama i časopisima bioloških nauka ... Pogledajte spisak i opis naših komercijalnih usluga ... Ministarstvo Nauke Beogradski Univerzitet ICGEB ostali ... "); array_files[126]=new Array(0,1,"http://www.imgge.bg.ac.yu/Srb/Laboratorije/06/lab.htm","2009-08-02","13K","IMGGI ::Laboratorija za molekularnu genetiku industrijskuh mikroorganiizama ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGI ::Laboratorija za molekularnu genetiku industrijskuh mikroorganiizama Pocetna > Laboratorije Naučnoistraživački interes: MOLEKULARNA GENETIKA INDUSTRIJSKIH MIKROORGANIZAMA (Bakterije mlečne kiseline, Pseudomonas sp. i Bacillus sp.) U našoj laboratoriji radi 8 Dr, 4 Mr i jedan BSc ... Završeni i aktivni projekti, istraživački uspesi ... Naši projekti objavljeni u arhivama i časopisima bioloških nauka ... See the list i description of our commercial services ... Ministarstvo Nauke Beogradski Univerzitet ICGEB ostali ... "); array_files[127]=new Array(0,1,"http://www.imgge.bg.ac.yu/Srb/Laboratorije/06/staff.htm","2009-08-02","16K","IMGGI :: Osoblje ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGI :: Osoblje Pocetna > Laboratorije > Lab. za M. Genetiku Industrijskih Mikroorganizama Rukovodilac laboratorije : Dr. Ljubiša TOPISIROVIĆ, redovni profesor Zamenik: Dr. Ivana STRAHINIĆ, viši naučni saradnik Istraživački tim : Dr. Milan KOJIĆ, naučni savetnik Dr. Jelena BEGOVIĆ, istraživač saradnik Dr. Đorđe FIRA, docent /BF Mr. Branko JOVČIĆ, istraživač saradnik Dr. Nataša GOLIĆ, viši naučni saradnik Mr. Katarina VELJOVIĆ, istraživač saradnik Dr. Amarela TERZIĆ VIDOJEVIĆ, naučni saradnik Mr. Milica NIKOLIĆ, istraživač saradnik Dr. Jelena LOZO, asistent /BF Mr. Maja TOLINAČKI, istraživač saradnik Završeni i aktivni projekti, istraživački uspesi ... Naši projekti objavljeni u arhivama i časopisima bioloških nauka ... Pogledajte spisak i opis naših komercijalnih usluga ... Ministarstvo Nauke Beogradski Univerzitet ICGEB ostali ... "); array_files[128]=new Array(0,1,"http://www.imgge.bg.ac.yu/Srb/Laboratorije/06/publications.htm","2009-08-02","16K","IMGGI :: Publikacije ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGI :: Publikacije Pocetna > Laboratorije > Lab. za M. Genetiku Industrijskih Mikroorganizama ben Omar, N., Valenzuela, A. S., Abriouel, H., López, R. L., Veljovic, K., Caňamero, M. M., Kojic, M., Topisirovic, L., Gálvez, A.: Virulence factors, antibiotic resistance, i bacteriocins in enterococci from artisan foods of animal origin. Food Control 20 (2009) 381-385. Strahinic, I., Kojic, M., Tolinacki, M., Fira, D. i Topisirovic, L. Molecular characterization of plasmids pS7a i pS7b from Lactococcus lactis subsp. lactis bv. diacetylactis S50 as a base for the construction of mobilizable cloning vectors. J. Appl. Microbiol. 106 (2009) 78-88. Lozo, J., Begovic, J., Jovcic, B., Golic, N., Topisirovic, L.: Effect of methionine i cysteine deprivation on the growth of different natural isolate of Lactobacillus spp. in chemically defined media. Arch. Biol. Sci. 60 (2008) 509-517. Nikolic, M., Terzic-Vidojevic, A., Jovcic, B., Begovic, J., Golic, N., Topisirovic, L.: Phenotypic i genotypic characterization of the microflora in the autochthonous homemade semi hard goats milk cheese. Int. J. Food Microbiol. 122 (2008) 162-170. Kojic, M., Jovcic, B., Begovic, J.,  Fira, D., Topisirovic, L.: Large chromosomal inversion correlated with spectinomycin resistance in Lactococcus lactis subsp. lactis bv. diacetylactis S50. Can. J. Microbiol. 54 (2008) 143-149. Busarcevic, M., Kojic M., Dalgalarrondo, M., Chobert, J. M., Haertlé, T., Topisirovic, L.: Purification of bacteriocin LS1 produced by human oral isolate Lactobacillus salivarius BGHO1. Oral. Microbiol. Immunol. 23 (2008) 254-258. Savic, D., Jokovic, N., Topisirovic, L.: Multivariate statistical methods in discrimination of lactobacilli based on their FTIR spectra. Dairy Sci. Tech. 88 (2008) 273-290. Jokovic, N., Nikolic, M., Begovic, J., Jovcic, B., Savic, D. i Topisirovic, L.: A survey of the lactic acid bacteria isolated from Serbian artisanal dairy product kajmak. Int. J Food Microbiol. 127 (2008) 305-311. Jovcic, B., Begovic, J., Lozo, J., Topisirovic, L., Koji "); array_files[129]=new Array(0,1,"http://www.imgge.bg.ac.yu/Srb/Laboratorije/06/projects.htm","2009-08-02","26K","IMGGI :: Projekti ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGI :: Projekti Pocetna > Laboratorije > Lab. za M. Genetiku Industrijskih Mikroorganizama Projekti: Razvoj probiotičkog preparata za humanu upotrebu na bazi bakterija mlečne kiseline (Inovacioni projekt - finansiran od strane MNTRRS, 2008-2009) Molecular characterisation of bacteriocin producing strains - Projekt Br.: CRP/YUG06-01 (finansiran od strane ICGEB, Trieste, Italy, 2007-2009) Lactobacilles - changements des propriétés allergenes des produits laitiers fermentés et caracterisation des bactériocines (finansiran od strane Yugoslav-France Bilateral Scientific Collaboration “Pavle Savic”, 2006-2007) Regulation of gene expression in stationary phase in Pseudomonas - Projekt Br.: CRP/YUG02-01 (finansiran od strane ICGEB, Trieste, Italy, 2003-2005) Izučavanje regulacije ekspresije gena odabranih industrijskih mikroorganizama (finansiran od strane MNZZSRS, 2006-2010) Molekularna genetika bakterija mlečne kiseline (BMK) (finansiran od strane MNTRRS 2003-2005) Molekularna genetika bakterija mlečne kiseline (BMK) (finansiran od strane MNTRRS, 2001-2003) Razvoj i unapredjenje tehnologije za proizvodnju visokovrednih prehrambenih proizvoda (VPP) iz domacih sirovina - Potprojekt: Standardizacija i unapredjenje tehnologije proizvodnje somborskog sira (Strateški-tehnološki projekt, finansiran od strane MNTRS, 1997-2000) Characterisation of plasmids from natural isolates of Lactobacilli useful for vector construction (finansiran od strane Yugoslav-Bulgarian Bilateral Scientific Collaboration, 1998-1999) Izolacija i karakterizacija bakterija mlečne kiseline za razvoj tehnološkog postupaka za proizvodnju specifičnog kopaoničkog sira (Inovacioni projekt - finansiran od strane MNTRS, 1998-1999) Izučavanje strukture i funkcije gena industrijskih mikroorganizama (finansiran od strane MSTRS, 1996-2000) Razvoj tehnološkog postupka za proizvodnju autohtonih sireva (Inovacioni projekt - MNTRS, 1994-1995) Primena genetičkog inženjerstva u konstrukciji starter kultura za industriju mleka (finansiran od strane SANU, 1990-1 "); array_files[130]=new Array(0,1,"http://www.imgge.bg.ac.yu/Srb/Laboratorije/08/lab.htm","2009-08-02","13K","IMGGI :: Laboratorija za molekularnu biologiju biljaka ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGI :: Laboratorija za molekularnu biologiju biljaka Pocetna > Laboratorije Osnovni naučni interes je proučavanje odabranih gena za proteine koji potencijalno učestvuju u fiziološkim procesima kao što su degradacija proteina i/ili odgovor na stres kod biljaka [Aspartične proteinaze (APs) i metalotioneini (MT) kod heljde]. Odgovor na stres - proces koji je specifičan za biljke kao sesilne organizme analizira se na dva sistema - heljdi (abiotički stress UV-B, suša, hladnoća, NaCl, AlCl3, teški metali…) i na poikilohidričnim biljkama Ramonda nathaliae i Ramonda serbica. Od interesa je i da razjasnimo glavne «aktere» kod samo-inkompatibilnog sistema heljde. U našoj laboratoriji radi 5 Dr, 5 Mr i 3 BSc ... Završeni i aktivni projekti, istraživački uspesi ... Naši projekti objavljeni u arhivama i časopisima bioloških nauka ... See the list i description of our commercial services ... Ministarstvo Nauke Beogradski Univerzitet ICGEB ostali ... "); array_files[131]=new Array(0,1,"http://www.imgge.bg.ac.yu/Srb/Laboratorije/08/staff.htm","2009-08-02","17K","IMGGI :: Osoblje ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGI :: Osoblje Pocetna > Laboratorije > Laboratorija za Molekularnu Biologiju Biljaka Rukovodilac laboratorije : Dr. Vesna MAKSIMOVIĆ, naučni savetnik Saradnici : Dr. Svetlana RADOVIĆ, vanredni profesor BU Bojana BANOVIĆ, istraživač saradnik, student doktorskih studija Dr .Miroslav KONSTANTINOVIĆ, naučni savetnik Živko JOVANOVIĆ, istraživač saradnik, student doktorskih studija Dr. Jovanka MILJUŠ ĐUKIĆ, viši naučni savetnik Na usavršavanju van zemlje : Mr. Jelena SAMARDŽIĆ, istraživač savetnik Dr. Jelena BRKLJAČIĆ, Plant Biotech. Center,Columbus, USA Mr. Gordana TIMOTIJEVIĆ, istraživač savetnik Mr. Nada ŠURBANOVSKI, East Malling Research, UK Mr. Dragana MAJIĆ, istraživač savetnik Ana BRATIĆ, Karolinska Institute, Stockholm, Sweden Mr. Mira MILISAVLJEVIĆ, istraživač savetnik Završeni i aktivni projekti, istraživački uspesi ... Naši projekti objavljeni u arhivama i časopisima bioloških nauka ... Pogledajte spisak i opis naših komercijalnih usluga ... Ministarstvo Nauke Beogradski Univerzitet ICGEB ostali ... "); array_files[132]=new Array(0,1,"http://www.imgge.bg.ac.yu/Srb/Laboratorije/08/publications.htm","2009-08-02","16K","IMGGI :: Publikacije ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGI :: Publikacije Pocetna > Laboratorije > Laboratorija za Molekularnu Biologiju Biljaka Ana M. Bratić, Dragana B. Majić, Jelena T. Samardžić i Vesna R. Maksimović, Functional analysis of the buckwheat metallothijedanin promoter: tissue specificity pattern i up-regulation under complex stress stimuli, J. Plant Phys. doi:10.1016/j.jplph.2008.12.002 2009 Bojana Banovic, Nada Surbanovski, Miroslav Konstantinovic, Vesna Maksimovic, Basic RNases of wild almond (Prunus webbii): Cloning i characterization of six new S-RNase i jedan ‘‘non-S RNase’’ genes, J. Plant Phys. 166, 395-402, 2008. M. Milisavljević, G. Timotijević, S. Radović, M. Konstantinović i V. Maksimović. Two types of aspartic proteinases from buckwheat seed–gene structure i expression analysis. J. Plant Phys. 165, 983-990 (2008) Nada Surbanovski, Kenneth R. Tobutt, Miroslav Konstantinovic, Vesna Maksimovic, Daniel J. Sargent, Vladimir Stevanovic, Encarnacion Ortega, Radovan I. Boskovic) Self-incompatibility of Prunus tenella i evidence that reproductively isolated species of Prunus have different SFB alleles coupled with an identical S-RNase allele. The Plant Journal 50, 723–734 (2007) Živko S. Jovanović , Jelena D. Milošević, Svetlana R. Radović Antioxidative enzymes in the response of buckwheat ( Fagopyrum esculentum Moench) to UV-B radiation , J. Agric. Food Chem., 54, 9472-9478(2006) Brkljačić J., Majić D., M iljuš -Đ ukić J., Bratić A., Konstantinović M., Milisavljevi ć M. i Vesna R. Maksimović The metallothijedanin-like gene from buckwheat – structural i functional analysis of the promoter region. Int. J. Plant Sci., 166: 889-900 (2005) Milisavljevic MD, Konstantinovic MM, Brkljacic JM, Maksimovic VR. Isolation i computer analysis of the 5 -regulatory region of the seed storage protein gene from buckwheat ( Fagopyrum esculentum Moench) J. Agric. Food Chem. 53: 2076-2080 (2005) Milisavljevic, M., Timotijević, G., Radović, S., Brkljačić, J., Konstantinović, M., Maksimović, V. Vicilin-like storage globulin from buckwheat seed. J. Agric. Food "); array_files[133]=new Array(0,1,"http://www.imgge.bg.ac.yu/Srb/Laboratorije/08/projects.htm","2009-08-02","21K","IMGGI :: Projekti ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGI :: Projekti Pocetna > Laboratorije > Laboratorija za Molekularnu Biologiju Biljaka Postojeći projekat Struktura, funkcija i regulacija ekspresije odabranih biljnih gena – osnovna istraživanja finansirana od Ministarstva za nauku i tehnološki razvoj Republike Srbije (2006-2010). Projekat BL143017 Prethodni projekti Analiza ekspresije odabranih biljnih gena – put do funkcije i biotehnološke primene – osnovna istraživanja finansirana od Ministarstva za nauku Republike Srbije (2002-2005) BL143051 Analiza genetičkog diverziteta divlje vrste Prunus u Srbiji i Crnoj Gori. Proučavanje molekularne osnove samo-inkompatibilnosti kod divlje vrste Prunus tenella, Prunus Webii, u sklopu projekta osnovnih istraživanja. Deo projekta je realizovan u saradnji sa East Malling Research, East Malling, UK (2005) Unapredjenje tehnologije gajenja voća i vinove loze (primenjena istraživanja finansirana od Ministarstva za nauku Republike Srbije u saradnji sa East Malling Research, East Malling, UK (2002-2004) Razvoj RT-PCR metode za identifikaciju RNK virusa voća. Saradnja sa Institutom za istraživanja u poljoprivredi ”Srbija” (1998-2001) Specifičnost tekućih projekata: 1.Analiza odabranih biljnih gena i regulacija njihove ekspresije Model-biljka u ovom projektu je heljda (Fagopyrum esculentum Moench). Analiza ekspresije specifičnih gena heljde je element u procesu razumevanja opštih mehanizama regulacije u biljnim ćelijama. To će takodje biti i osnova u pojašnjavanju moguće funkcije proteina kodiranih odabranim genima u različitim fiziološkim procesima i uslovima stresa kod heljde. Od posebnog interesa je regulacija ekspresije dva tipa gena/proteina: Metalotioneini (MTs) – Iako su MTs nadjeni u gotovo svim živim organizmima, a njihova struktura, lokalizacija i regulacija ekspresije gena intenzivno proučavana, specifična funkcija u različitim fiziološkim stanjima i uslovima stresa, ostaje otvoreno polje istraživanja. Na osnovu visokog kapaciteta za vezivanje jona metala, pretpostavlja se da su odgovorni za homeostazu metala "); array_files[134]=new Array(0,1,"http://www.imgge.bg.ac.yu/Srb/Services/interferon.htm","2009-08-02","15K","IMGGI :: Interferons ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGI :: Interferons Pocetna > Commercial services Interferons are proteins able to mediate antiviral, anti-proliferative i immunomodulatory activities in response to a viral infection or other biological inducers. Recently an increasing number of clinical studies report on use of IFNß in successful treatment of multiple sclerosis (MS), viral hepatitis, human papilloma virus i in conventional fractionated radio therapy. On the world market there are two recombinant IFNß, jedan produced in bacteria E.coli (“BETASERON”, without first methionine i sugars i with jedan amino acid alteration) i the second in Chinese hamster ovary cells (“AVONEX”, high cost of cultivation). Betaseron costs approximately 1,000 for a monthly tretman of patients with relapsing-remitting MS ( 250µg/dose of Betaseron with a biological activity of 2xl0 7 IU/mg, patients receives 3 doses/week). Human IFNß gene was expressed in following expression systems: 1. E. coli (soluble, intracellular) – 10 6 U /l. 2. Saccharomyces cerevisiae ( episomal multi-copy expression vector ) Shake flask grown cells secretes recombinant human IFNß( rHuIFN- b ) into the medium at the level of 5x10 6 U/l (4). 3. P. pastoris (low- copy homologous integration ) rHuIFN- b was secreted from shake flask-grown P. pastoris cells into the medium at a level of 3x 10 8 U/l. This yield obtained in non optimised cultivation is higher by an order of magnitude than that obtained in the E. coli expression system. Biological activity was measured by inhibition of cytopathic effect of vesicular stomatitis virus on Wish cells. The ability of P. pastoris to grow to high densities in a fermentor together with the selection of rHuIFN- b multicopy P. pastoris cljedan would enable us even a larger production of functionally active rHuIFN- b . The primary structure of the rHuIFN beta (166 amino acids) is identical to that of natural human interferon beta, as determined by N-terminal amino acids sequencing. References: •  Todorović, V., Skoko, N., Pavković, N., Grujičić, N., Zarić, J "); array_files[135]=new Array(0,1,"http://www.imgge.bg.ac.yu/Srb/Services/gmo.htm","2009-08-02","14K","IMGGI :: GMO ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGI :: GMO Pocetna > Commercial services At the Laboratory for Plant Molecular Biology, the PCR METHOD for DETECTION, IDENTIFICATION i QUANTIFICATION OF GENETIC MODIFICATION IN PLANT MATERIAL AND FOOD/FEED PRODUCTS has been developed i stiardized. The IMGGE is authorized by Ministry of Agriculture of Republic of Serbia as an official institution for GMO testing. The Laboratory is involved in International Quality Control system organized by International Seed Testing Association (ISTA proficiency test) Method comprises isolation of DNA i PCR tests: Qualitative a) General GMO screening (presence of CaMV35S promoter i NOS terminator)  b) Test specific for defined genetic modifications -RoundUp Ready soybean i corn NK603 (herbicide tolerant) -Liberty Link (T25) corn (herbicide tolerant) -Bt corn: Bt176-Bt11 i MON810 (insect resistant) Quantitative Real Time PCR test (Applied Biosystems, ABI Prism 7500) for quantification of genetic modification. METHOD IS ACCURATE AND SENSITIVE Detection limit is 1pg of DNA, enabling detection of genetic modification in: · samples of only 0.01% GMO (jedan modified out of 10,000) · food i feed products: lecithin, flour, chops, flakes, soybean meal, soybean protein isolates… The method is stiardized according to criteria for accredited laboratories in Europe. CONTACT: Vesna Maksimović, Ph. D. TEL: ++ 381 11 3976 414 FAX: ++ 381 11 3975 808 e-mail: vesamax@imgge.bg.ac.rs Upravni odbor, Direktor, Zamenik direktora, Naučno veće, Laboratorije ... Predsednik Upravnog odbora, Sekretar za finansije ... Do nas možete doći autobuskim linijama : 33, 39 ili 25 ... Pogledajte naše slike ... Ministry of Science Belgrade University ICGEB more links ... "); array_files[136]=new Array(0,1,"http://www.imgge.bg.ac.yu/Srb/Services/fish.htm","2009-08-02","13K","IMGGI :: FISH ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGI :: FISH Pocetna > Komercijalni servisi Dijagnostika DiGeorge sindroma (mikrodelecija 22q11.2) Razvijena je i primenjena FISH metoda za prenatalnu i postnatalnu detekciju hromozomskih aberacija kod čoveka za: Dijagnostiku mikrodelecionih sindroma (DiGeorge i Prader-Willi / Angelman) Odredjivanje najčešćih numeričkih aberacija hromozoma 13,18, 21, X i Y Detekciju marker hromozoma Detekciju hromozomskog mozaicizma KONTAKT: Dr. Milena Stevanovic TEL: ++ 381 11 3976 212 FAX: ++ 381 11 3975 808 e-mail: stevanov@eunet.rs Upravni odbor, Direktor, Zamenik direktora, Naučno veće, Laboratorije ... Predsednik Upravnog odbora, Sekretar za finansije ... Do nas možete doći autobuskim linijama : 33, 39 ili 25 ... Pogledajte naše slike ... Ministry of Science Belgrade University ICGEB more links ... "); array_files[137]=new Array(0,1,"http://www.imgge.bg.ac.yu/Srb/Services/blad.htm","2009-08-02","14K","IMGGI :: BLAD ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGI :: BLAD Pocetna > Komercijalni servisi s) standard Phi X/HaeIII k) nesečeni PCR produkt z) zdrava životinja h) heterozigotni nosilac BLAD (Bovine Leukocyte Adhesion Deficiency) je letalno, autozomalno recesivno oboljenje Holštajn-Frizijske rase goveda. Obolela goveda su podložna infekcijama koje se vraćaju nakon konvencionalnog lečenja, tako da goveda obično umiru pre dostizanja polne zrelosti. Bolest je uzrokovana tačkastom mutacijom u genu za CD18 površinski glukoproteinski receptor leukocita. Ova mutacija dovodi do promene u receptornom proteinu, što sprečava migraciju neutrofila kroz vaskularni endotelijum do ekstravaskularnih tkiva. Posledica je umanjena otpornost životinja prema infektivnim bolestima. PCR-RFLP DNK test omogućava brzo i efikasno detektovanje mutacije u CD18 genu. KONTAKT: Jelena Popovic, Mr TEL: ++ 381 11 3976 212 FAX: ++ 381 11 3975 808 e-mail: jpopovic@imgge.bg.ac.rs Upravni odbor, Direktor, Zamenik direktora, Naučno veće, Laboratorije ... Predsednik Upravnog odbora, Sekretar za finansije ... Do nas možete doći autobuskim linijama : 33, 39 ili 25 ... Pogledajte naše slike ... Ministry of Science Belgrade University ICGEB more links ... "); array_files[138]=new Array(0,1,"http://www.imgge.bg.ac.yu/Srb/Services/tptm.htm","2009-08-02","14K","IMGGI :: TPMT ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGI :: TPMT Pocetna > Commercial services Thiopurine S-methyltransferase (TPMT) is a cytosolic enzyme that catalyzes S-methylation (inactivation) of thiopurine drugs such as anticancer (6-mercaptopurine i 6-thioguanine) i immunosupressant agents (azathioprine). These drugs are widely used for treatment of: 1. cancers 2. leukemias 3. autoimmune diseases 4. inflammatory bowel disease (ulcerous colitis, Crohns disease) Decreased activity of TPMT is associated with severe hematopoietic toxicity after using stiard doses of these drugs. Moreover, this toxicity can be fatal. There are several mutations in TPMT gene which give rise to low phenotypic activity. The doses of administered thiopurine drugs are reduced regarding patients genotype character. After that, patients do not develop hematopoietic toxicity complications. Mutations detected in the Laboratorija za Molekularnu Hematologiju by DNA analysis are: TPMT(G238C), TPMT (G460A) i TPMT (A719G). CONTACT: Dr Sonja Pavlović TEL: ++ 381 11 3976 445 FAX: ++ 381 11 3975 808 e-mail: sonya@sezampro.rs Upravni odbor, Direktor, Zamenik direktora, Naučno veće, Laboratorije ... Predsednik Upravnog odbora, Sekretar za finansije ... Do nas možete doći autobuskim linijama : 33, 39 ili 25 ... Pogledajte naše slike ... Ministry of Science Belgrade University ICGEB more links ... "); array_files[139]=new Array(0,1,"http://www.imgge.bg.ac.yu/Srb/Services/thalassemia.htm","2009-08-02","14K","IMGGI :: Thalassemia ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGI :: Thalassemia Pocetna > Commercial services The thalassemia syndromes The thalassemia syndromes are a heterogeneous group of inhereted anemias characterized by defects in the synthesis of jedan or more of the globin chain subunits of the hemoglobin tetramer. The thalassemias are inherited as pathologic allels of jedan or more of the globin genes. Clinical manifestations are diverse, ranging from asymptotic hypochromia i microcytosis to profound anemia, which is fatal in utero or in early childhood if untreated. When bjedan marrow transplantation is performed early in life there is a great probability of cure. The thalassemias are most common in the Mediterranean basin i equatorial or near-equatorial regions of Asia i Africa. FR Yugoslavia belongs to thalassemia belt where frequencies of pathologic genes are within the range of 2.5-15%. Molecular diagnostic of thalassemia is the most reliable method for: accurate diagnosis early in life. Modern therapy initiated in early stage prolonges survival of these patients. prenatal diagnosis, which decreases the incidence of thalassemia patient births. In Laboratory for molecular hematology the modern methods are used for diagnostic of thalassemia, including hemoglobin analysis (electrophoresis of Hb) i globin genes analysis ( reverse dot-blot, PCR). CONTACT: Dr Sonja Pavlović TEL: ++ 381 11 3976 445 FAX: ++ 381 11 3975 808 e-mail: sonya@sezampro.rs Upravni odbor, Direktor, Zamenik direktora, Naučno veće, Laboratorije ... Predsednik Upravnog odbora, Sekretar za finansije ... Do nas možete doći autobuskim linijama : 33, 39 ili 25 ... Pogledajte naše slike ... Ministry of Science Belgrade University ICGEB more links ... "); array_files[140]=new Array(0,1,"http://www.imgge.bg.ac.yu/Srb/Services/sry.htm","2009-08-02","13K","IMGGI :: SRY ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGI :: SRY Pocetna > Komercijalni servisi s) standard Phi X/HaeIII k) negativna kontrola ?)pacijent sa reverzijom pola, ženski fenotip ž)pozitivna kontrola ženskog pola m)pozitivna kontrola muškog pola Razvijena je metoda bazirana na PCR-u za detekciju SRY gena. Metoda omogućuje dijagnostiku reverzije pola (interseksualnosti) koja je povezana sa povećanim rizikom pojave gonadalnih tumora tipa disgerminoma i gonadoblastoma. KONTAKT: Dr. Milena Stevanovic TEL: ++ 381 11 3976 212 FAX: ++ 381 11 3975 808 e-mail: stevanov@eunet.rs Upravni odbor, Direktor, Zamenik direktora, Naučno veće, Laboratorije ... Predsednik Upravnog odbora, Sekretar za finansije ... Do nas možete doći autobuskim linijama : 33, 39 ili 25 ... Pogledajte naše slike ... Ministry of Science Belgrade University ICGEB more links ... "); array_files[141]=new Array(0,1,"http://www.imgge.bg.ac.yu/Srb/Services/porcine.htm","2009-08-02","14K","IMGGI :: Stresni sindrom svinja ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGI :: Stresni sindrom svinja Pocetna > Komercijalni servisi s) standard Phi X/HaeIII k) nesečeni PCR produkt T/T) bolesna životinja C/T) heterozigotni nosilac C/C)zdrava životinja Maligna hipertermija, poznatija kao stresni sindrom svinja, je nasledno, autozomalno, recesivno oboljenje koje se javlja kod pojedinih selekcionisanih rasa svinja. Osnovna manifestacija ovog sindroma je iznenadno uginuće izazvano stresom (loši uslovi gajenja, transport, parenje) koje je praćeno pojavom bledog i vodnjikavog mesa kod svinja koje su osetljive na stres.        Analiza sekvence gena za rianodinski receptor (RYR1) je pokazala da mutacija na poziciji 1843 dovodi do ukidanja mesta koje prepoznaje restrikcioni enzim HinP1I. To otvara mogućnost primene neinvazivnog DNK testa zasnovanog na lančanoj reakciji umnožavanja DNK (PCR) i analizi polimorfizma restrikcionih fragmenata (RFLP). Ovaj DNK test omogućava pouzdano odredjivanje normalnog i mutiranog alela, a samim tim i identifikaciju zdravih svinja (C/C), svinja osetljivih na stres (T/T), kao i heterozigotnih nosioca (C/T). KONTAKT: Jelena Popovic, Mr TEL: ++ 381 11 3976 212 FAX: ++ 381 11 3975 808 e-mail: jpopovic@imgge.bg.ac.rs Upravni odbor, Direktor, Zamenik direktora, Naučno veće, Laboratorije ... Predsednik Upravnog odbora, Sekretar za finansije ... Do nas možete doći autobuskim linijama : 33, 39 ili 25 ... Pogledajte naše slike ... Ministry of Science Belgrade University ICGEB more links ... "); array_files[142]=new Array(0,1,"http://www.imgge.bg.ac.yu/Srb/Services/pku.htm","2009-08-02","13K","IMGGI :: PKU ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGI :: PKU Pocetna > Commercial services Phenylketonuria (PKU) is the most common inborn error of amino acid metabolism in Caucasians. It is caused by an autosomal recessive deficiency of the hepatic enzyme phenylalanine hydroxylase (PAH). Inability of phenylalanine conversion to tyrosine results in an increase of phenylalanine in body fluids i severe mental retardation unless the dietary phenylalanine intake is restricted. In the Laboratorija za Molekularnu Hematologiju testing for PKU is djedan by DNA analysis. Identifying the disease-causing mutation is useful for: refining diagnosis anticipating dietary requirements genetic counseling CONTACT: Dr Sonja Pavlović TEL: ++ 381 11 3976 445 FAX: ++ 381 11 3975 808 e-mail: sonya@sezampro.rs Upravni odbor, Direktor, Zamenik direktora, Naučno veće, Laboratorije ... Predsednik Upravnog odbora, Sekretar za finansije ... Do nas možete doći autobuskim linijama : 33, 39 ili 25 ... Pogledajte naše slike ... Ministry of Science Belgrade University ICGEB more links ... "); array_files[143]=new Array(0,1,"http://www.imgge.bg.ac.yu/Srb/Services/penicillin.htm","2009-08-02","16K","IMGGI :: Penicillin ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGI :: Penicillin Pocetna > Commercial services Penicillin G amidase (E.C. 3.5.1.11; PAC) catalyses the hydrolysis of the production of semisynthetic beta-lactam antibiotics. It is jedan of the most widely used enzymes at an the acyl group of the penicillin G, yielding 6-aminopenicillanic acid (6-APA), a key intermediate in industrial scale whose market is immediately after glucose isomerase. The world manufacturing of the immobilized enzyme is around 10 tjedan per year i 7500 tjedan of 6APA. Production of 1kg 6APA (1500 ) needs 1kg of penicillin (10 ) i 2,8g of enzyme penicillin G amidase (650 ). The P.rettgeri pac gene was expressed in following expression systems: Saccharomyces cerevisiae (episomal multi-copy expression vector) Shake flask culture produce ~ 1.5U/ml (1). The enzyme is secreted into the medium. Batch fermentation expression level is 5.7U/ml. The PAC yield is significantly increased by more than 500-fold compared to that obtained in the native bacterium, i the recombinant enzyme is almost entirely secreted (2). Recombinant PAC is purified by a combination of hydrophobic interaction i ion-exchange chromatography. •  Pichia pastoris (integration expression vector) , low i multi-copy homologous integration, produce 0.18U/ml (3) i 2.4U/ml respectively in shake flask culture in non optimised conditions. Recombinant PAC produced in P.pastoris is highly thermostable compared to the jedan produced in S.cerevisiae. References: •  Ljubijankić, G., Konstantinović, M. i Glišin, V. 1992. The primary structure of Providencia rettgeri penicillin G amidase gene i its relationship to other Gram negative amidases. DNA Sequence-J. DNA Sequencing i Mapping 3: 195-200. •  Konstantinović, M., Marjanović, N., Ljubijankić, G. i Glišin, V. 1994. The penicillin amidase of Arthrobacter viscosus (ATCC 15294). Gene 143: 79-83. •  Carlo Bruschi i Goran Ljubijankić, 1995. The method for the production of Penicillin G amidases in yeast Saccharomyces cerevisiae , Italian patent application MI95-A001083. •  Ljubijankić, G "); array_files[144]=new Array(0,1,"http://www.imgge.bg.ac.yu/Srb/Services/pcr.htm","2009-08-02","14K","IMGGI :: PCR ","imgge, Institute, Molecular, genetics, genetic, engineering, Beograd, Belgrade, biology, imggi ",""," IMGGI :: PCR Pocetna > Komercijalni servisi k-CASEIN s) standard Phi X/HaeIII k) nesečeni PCR produkt AB) heterozigot AA) homozigot za varijantu A BB) homozigot za varijantu B ß- LACTOGLOBULIN s) standard Phi X/HaeIII k)nesečeni PCR produkt AB) heterozigot AA) homozigot za varijantu A BB) homozigot za varijantu B Tehnika lančanog umnožavanja DNK (PCR) i polimorfizam dužine restrikcionih fragmenata (RFLP) korišćene su za određivanje genetičkih varijanti proteina mleka. Određivanje varijanti k-kazeina i b-laktoglobulina i selekcija poželjnih genotipova ima veliki ekonomski značaj jer varijante ovih proteina utiču na prerađivačke osobine mleka. KONTAKT: Jelena Popovic, Mr TEL: ++ 381 11 3976 212 FAX: ++ 381 11 3975 808 e-mail: jpopovic@imgge.bg.ac.rs Upravni odbor, Direktor, Zamenik direktora, Naučno veće, Laboratorije ... Predsednik Upravnog odbora, Sekretar za finansije ... Do nas možete doći autobuskim linijama : 33, 39 ili 25 ... Pogledajte naše slike ... Ministry of Science Belgrade University ICGEB more links ... "); array_files[145]=new Array(0,4,"http://www.imgge.bg.ac.yu/Files/cenovnik.pdf","2009-07-20","34K"," Untitled-1.html ","","","R.broj 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 Naziv usluge FISH na preparatima periferne krvi (numericke aberacije hromozoma 1,13/21,18, X i Y) FISH na histoloskim preparatima Obelezavanje proba za flourescentnu hibridizaciju (10 plocica) Detekcija delecije 22q11 primenom FISH-a Kvalitativni PCR - Detekcija CaMV 35S promotora NOS terminatora »Round Up Ready« konstrukta u materijalu koji sadrzi DNK soje Kvalitativni PCR - Detekcija CaMV 35S promotora »Round Up Ready« konstrukta i genskih konstrukata karakteristicnih za LibertyLink-T25, Bt11 i MON810 u materijalu koji sadrzi DNK kukuruza Izdavanje GMO nalaza na engleskom jeziku Kvantifikacija prisustva geneticki modifikovanog materijala Analiza varijanti gena za kapa-kazein goveda Analiza varijanti gena za beta-laktoglobulin goveda Detekcija SRY gena na uzorku periferne krvi Detekcija SRY gena na uzorku plodove vode Detekcija SRY gena na uzorku gonadalnog tkiva Kvantifikacija prisustva geneticki modifikovanog materijala Molekularna dijagnostika cisticne fibroze (7 najcecih mutacija) Prenatalna dijagnostika cisticne fibroze Molekularna dijagnostika S i Z mutacija A1AT gena Pretrazivanje celog CFTR gena 1 (egzoni: 1,3,4,5,6a,7,9,10, 11,12,13,14a,15,17b,19,20) Pretrazivanje celog CFTR gena2(egzoni 2,6b,8,14b,16,17a,18,21, 22,23,24) Molekularna dijagnostika mutacije FV Leiden Molekularna dijagnostika mutacije protrombin FII G20210A Molekularna dijagnostika mutacije MTHFR C677T Molekularna dijagnostika mutacije FV i FII Molekularna dijagnostika mutacije FV i MTHFR Molekularna dijagnostika mutacije FII i MTHFR Molekularna dijagnostika mutacije FV, FII i MTHFR Molekularna dijagnostika CFTR F508del mutacije Detekcija mutacija u genu za TPMT (izbor terapijskih protokola Za ALL, AML, kancer, auto-imune bolesti) Molekularna dijagnostika BLAD-a Molekularna dijagnostika stresnog sindroma svinja Molekularna dijagnostika alpha-talasemija Molekularna dijagnostika beta- tal "); array_files[146]=new Array(0,4,"http://www.imgge.bg.ac.yu/Srb/Files/svetskabanka.pdf","2009-07-18","3189K","svetskabanka.pdf ","",""," "); array_files[147]=new Array(0,4,"http://www.imgge.bg.ac.yu/Srb/Files/publications08.pdf","2009-07-18","33K"," Microsoft Word - Document1 ","","","Laboratory for Plant Molecular Biology - Publications ­ · Ana M. Brati, Dragana B. Maji, Jelena T. Samardzi and Vesna R. Maksimovi, Functional analysis of the buckwheat metallothionein promoter: tissue specificity pattern and up-regulation under complex stress stimuli, J. Plant Phys. doi:10.1016/j.jplph.2008.12.002 2009 · Bojana Banovic, Nada Surbanovski, Miroslav Konstantinovic, Vesna Maksimovic, Basic RNases of wild almond (Prunus webbii): Cloning and characterization of six new S-RNase and one ``non-S RNase genes, J. Plant Phys. 166, 395-402, 2008. · M. Milisavljevi, G. Timotijevi, S. Radovi, M. Konstantinovi and V. Maksimovi. Two types of aspartic proteinases from buckwheat seed­gene structure and expression analysis. J. Plant Phys. 165, 983-990 (2008) · Nada Surbanovski, Kenneth R. Tobutt, Miroslav Konstantinovic, Vesna Maksimovic, Daniel J. Sargent, Vladimir Stevanovic, Encarnacion Ortega, Radovan I. Boskovic) Self-incompatibility of Prunus tenella and evidence that reproductively isolated species of Prunus have different SFB alleles coupled with an identical S-RNase allele. The Plant Journal 50, 723­734 (2007) · Zivko S. Jovanovi , Jelena D. Milosevi, Svetlana R. Radovi Antioxidative enzymes in the response of buckwheat ( Fagopyrum esculentum Moench) to UV-B radiation , J. Agric. Food Chem., 54, 9472-9478(2006) · Brkljaci J., Maji D., M iljus - uki J., Brati A., Konstantinovi M., Milisavljevi M. and Vesna R. Maksimovi The metallothionein-like gene from buckwheat ­ structural and functional analysis of the promoter region. Int. J. Plant Sci., 166: 889900 (2005) · Milisavljevic MD, Konstantinovic MM, Brkljacic JM, Maksimovic VR. Isolation and computer analysis of the 5 -regulatory region of the seed storage protein gene from buckwheat ( Fagopyrum esculentum Moench) J. Agric. Food Chem. 53: 2076-2080 (2005) · Milisavljevic, M., Timotijevi, G., Radovi, S., Brkljaci, J., Konstantinovi, M., Maksimovi, V. Vicilin-like storage globulin from buckwheat seed. J. Agric. Food Chem. 52, 5258-5262 (2004) · Miljus-Djuki "); array_files[148]=new Array(0,4,"http://www.imgge.bg.ac.yu/Srb/Files/publications06.pdf","2009-07-18","54K"," Microsoft Word - Document1 ","","","Laboratory for Molecular Genetics of Industrial Microorganisms - Publications - · ben Omar, N., Valenzuela, A. S., Abriouel, H., López, R. L., Veljovic, K., Caamero, M. M., Kojic, M., Topisirovic, L., Gálvez, A.: Virulence factors, antibiotic resistance, and bacteriocins in enterococci from artisan foods of animal origin. Food Control 20 (2009) 381-385. Strahinic, I., Kojic, M., Tolinacki, M., Fira, D. and Topisirovic, L. Molecular characterization of plasmids pS7a and pS7b from Lactococcus lactis subsp. lactis bv. diacetylactis S50 as a base for the construction of mobilizable cloning vectors. J. Appl. Microbiol. 106 (2009) 78-88. Lozo, J., Begovic, J., Jovcic, B., Golic, N., Topisirovic, L.: Effect of methionine and cysteine deprivation on the growth of different natural isolate of Lactobacillus spp. in chemically defined media. Arch. Biol. Sci. 60 (2008) 509-517. Nikolic, M., Terzic-Vidojevic, A., Jovcic, B., Begovic, J., Golic, N., Topisirovic, L.: Phenotypic and genotypic characterization of the microflora in the autochthonous homemade semi hard goats milk cheese. Int. J. Food Microbiol. 122 (2008) 162-170. Kojic, M., Jovcic, B., Begovic, J., Fira, D., Topisirovic, L.: Large chromosomal inversion correlated with spectinomycin resistance in Lactococcus lactis subsp. lactis bv. diacetylactis S50. Can. J. Microbiol. 54 (2008) 143-149. Busarcevic, M., Kojic M., Dalgalarrondo, M., Chobert, J. M., Haertlé, T., Topisirovic, L.: Purification of bacteriocin LS1 produced by human oral isolate Lactobacillus salivarius BGHO1. Oral. Microbiol. Immunol. 23 (2008) 254-258. Savic, D., Jokovic, N., Topisirovic, L.: Multivariate statistical methods in discrimination of lactobacilli based on their FTIR spectra. Dairy Sci. Tech. 88 (2008) 273-290. Jokovic, N., Nikolic, M., Begovic, J., Jovcic, B., Savic, D. and Topisirovic, L.: A survey of the lactic acid bacteria isolated from Serbian artisanal dairy product kajmak. Int. J Food Microbiol. 127 (2008) 305-311. Jovcic, B., Begovic, J., Lozo, J., Topisirovic, L., Kojic, M.: Post "); array_files[149]=new Array(0,4,"http://www.imgge.bg.ac.yu/Srb/Files/publications05.pdf","2009-07-18","39K"," Microsoft Word - Document1 ","","","Laboratory for Molecular Genetics of Actinomycetes - Publications Ili ­ Tomi, T., Mori, I., Conn, G. L., Vasiljevi, B.: Aminoglycoside resistance genes sgm and kgmB protect bacterial but not yeast small ribosomal subunits in vitro despite high conservation of the rRNA site. Res. in Microbiol. 159 (2008) 658 ­ 662. · · Savi, M., Ili ­ Tomi, T., Macmaster, R., Vasiljevi, B., Conn, G. L.: Critical Residues for Cofactor Binding and Catalytic Activity in the Aminoglycoside Resistance Methyltransferase Sgm. J. Bacteriol. 190 (2008) 5855 ­ 5861. · Koji, M., Vojnovi, S., Vukov, N., Vasiljevi, B.: Overexpression of Sgm 5 UTR mRNA reduces gentamicin resistance in both Escherichia coli and Micromonospora melanosporea cells. Arch. Biol.Sci. 59 (2007) 273 ­ 280. · Savi, M., Brati I., Vasiljevi, B.: Streptomyces durmitorensis sp. nov., a producer of an FK506-like immunosuppressant. International Journal of Systematic and Evolutionary Microbiology 57 (2007) 2119-2124. · Savi, M., Vasiljevi, B.: Targeting polyketide synthase gene pool within actinomycetes: new degenerate primers. J. Ind. Microbiol. Biotechnol. 33 (2006) 423430. · Markovi, S., Vojnovi, S., Jovanovi, M., Vasiljevi, B.: Different expression level of two KgmB-His fusion proteins. Journal of the Serbian Chemical Society 70 (2005) 1401-1407. · Ili Tomi, T., Markovi, S., Vasiljevi, B.: Expression and purification of the Sgm protein from E. coli. Journal of the Serbian Chemical Society 70 (2005) 817-822. · Skoko, N., Vujovi, J., Savi, M., Papi, N., Vasiljevi, B., Ljubijanki, G.: Construction of Saccharomyces cerevisiae strain FAV20 useful in detection of immunosuppressants produced by soil actinomycetes. Journal of Microbiological Methods 61 (2005) 137-140. · Vaji, S., Anastasov, N., Vasiljevi, B.: The kgmB gene, encoding ribosomal RNA methylase from Streptomyces tenebrarius, is autogenously regulated. Archives of Microbiology. 182 (2004) 475-481. · Koji, M., Milojevi, N., Vasiljevi, B.: Gentamicin-resistance determinants confer background-dependent hygromycin B resis "); array_files[150]=new Array(0,4,"http://www.imgge.bg.ac.yu/Srb/Files/publications04.pdf","2009-07-18","52K"," Microsoft Word - Document1 ","","","Laboratory for Human Molecular Genetics - Publications Petrovi, I., Kovacevi-Grujici, N. and Stevanovi, M. ZBP-89 and Sp3 downregulate while NF-Y up-regulates SOX18 promoter activity in HeLa cells. 2008. Molecular Biology Reports, DOI 10.1007/s11033-008-9272-x. · · Kovacevi Grujici, N., Yokoyama, K. K. and Stevanovi, M. Trans-activation of the human SOX3 promoter by MAZ in NT2/D1 cells. 2008. Archives of Biological Sciences, Belgrade, 60, 379-387. · Nikcevi, G., Savi, T., Kovacevi-Grujici, N. and Stevanovi, M. Up-regulation of the SOX3 gene expression by retinoic acid: characterization of the novel promoterresponse element and the retinoid receptors involved. 2008. Journal of Neurochemistry, 107, 1206-1215. · Krsti, A., Mojsin, M., Kovacevi-Grujici, N. and Stevanovi, M. PCR amplification and sequence analysis of the rat Sox3 gene. 2008. Archives of Biological Sciences, Belgrade, 60, 525-530. · Djordjevi, V., Denci-Fekete, M., Jovanovi, J., Drakuli, D., Stevanovi, M., Jankovi, G. and Goti, M. Pattern of trisomy 1q in hematological malignancies: a single institution experience. 2008. Cancer Genet Cytogenet. 186, 12-18. · Cuturilo, G., Drakulic, D., Stevanovic, M., Jovanovic, I., Djukic, M., Miletic-Grkovic, S. and Atanaskovic-Markovic, M. A rare association of interrupted aortic arch type C and microdeletion 22q11.2. 2008. Eur J Pediatr. 167, 1195-1198. · Krsti, A., Mojsin, M. and Stevanovi, M. Regulation of SOX3 gene expression is driven by multiple NF-Y binding elements. 2007. Archives of Biochemistry and Biophysics, 467, 163-173. · Kovacevi Grujici, N., Mojsin, M., Djurovi, J., Petrovi, I. and Stevanovi, M. Comparison of promoter regions of SOX3, SOX14 and SOX18 orthologs in mammals. 2008. DNA Sequence, 19, 185-194. · Drakuli D., Nikcevi, G., Djordjevi, V. and Stevanovi M. Generation of a whole chromosome painting probe from monochromosomal hybrid cells by the Alu polymerase chain reaction.2007. Archives of Biological Sciences, 59, 89-95. · Nikcevi, G., Kovacevi Grujici, N., Stevanovi, M. Activation of the HSV "); array_files[151]=new Array(0,4,"http://www.imgge.bg.ac.yu/Srb/Files/publications03.pdf","2009-07-18","56K"," Microsoft Word - Document1 ","","","Laboratory for Molecular Biology - Publications · M. Stankovic, A. Nikolic, A. Divac, A. Tomovic, N. Petrovic-Stanojevic, M. Andjelic, V. Dopudja-Pantic, M. Surlan, I. Vujicic, D. Ponomarev, M. Mitic-Milikic, J. Kusic,D. Radojkovic. The CFTR M470V gene variant as a potential modifier of COPD severity ­ study of Serbian population. Genet Test. 2008;12(3):357 Stefanovic D, Kusic J, Divac A, Tomic B. Formation of noncanonical DNA structures mediated by human ORC4, a protein component of the origin recognition complex Biochemistry. 2008 Aug 19;47(33):8760-7. Zaimidou S, van Baal S, Smith TD, Mitropoulos K, Ljujic M, Radojkovic D, Cotton RG, Patrinos GP. (2008) A(1)ATVar: a relational database of human SERPINA1 gene variants leading to alpha(1)-antitrypsin deficiency and application of the VariVis software. Hum Mutat. 2008 Nov 19. [Epub ahead of print] Radojkovic M, Ristic S, Colovic M, Cemerikic-Martinovic V, Radojkovic D, Krtolica K. Molecular characteristics and prognostic significance of Bcl-2/IgH gene rearrangement in Serbian follicular lymphoma patients. Neoplasma. 2008; 55(5):4217. Castellani C, Cuppens H, Macek M Jr, Cassiman JJ, Kerem E, Durie P, Tullis E, Assael BM, Bombieri C, Brown A, Casals T, Claustres M, Cutting GR, Dequeker E, Dodge J, Doull I, Farrell P, Ferec C, Girodon E, Johannesson M, Kerem B, Knowles M, Munck A, Pignatti PF, Radojkovic D, Rizzotti P, Schwarz M, Stuhrmann M, Tzetis M, Zielenski J, Elborn JS. Consensus on the use and interpretation of cystic fibrosis mutation analysis in clinical practice. J Cyst Fibros. 2008 May;7(3):179-96. Review. Nisevic I, Dinic J, Nikolic A, Djordjevic V, Lukic S, Ugljesic M, Andjelic-Jelic M, Petrovic-Stanojevic N, Radojkovic D. MTHFR C677T polymorphism in chronic pancreatitis and pancreatic adenocarcinoma. Cell Biochem Funct. 2008;26(6):659-63. Ljujic M, Topic A, Divac A, Nikolic A, Petrovic-Stanojevic N, Surlan M, MiticMilikic M, Radojkovic D. Isoelectric focusing phenotyping and denaturing gradient gel electrophoresis genotyping: a comparison of two met "); array_files[152]=new Array(0,4,"http://www.imgge.bg.ac.yu/Srb/Files/publications01.pdf","2009-07-18","44K"," Microsoft Word - Document1 ","","","Laboratory for Molecular Hematology - Publications · Colovic M, Pavlovic S, Kraguljac N, Colovic N, Jankovic G, Sefer D, Tosic N. Acquired amegakaryocytic thrombocytopenia associated with proliferation of gamma/delta TCR T-lymphocytes and a BCR-ABL (p210) fusion transcript. Eur J Haematol. 2004 Nov;73(5):372-375. Poznani J, Perisi Lj, Urosevi J, Petrucev B, ureinovi T, Tosi N, KrivokapiDokmanovi L, Jani D, Cvorkov-Drazi M, Bunjevacki G, Pavlovi S. Biochemical phenotype and origin of the three most common beta-thalassemia mutations in Serbia. Yug Med Bioch 2004. Kollia P, Kalamaras A, Chassanidis C, Samara M, Vamvakopoulos NK, Radmilovi M, Pavlovi S, Papadakis MN, Patrinos GP. Compound heterozygosity for the Cretan type of non-deletional heriditary persistence of fetal hemoglobin and betathalassemia or Hb Sabine confirms the functional role of the A gamma -158 CT mutation in gamma-globin gene transcription. Blood Cells Mol Dis. 2008 Nov-Dec; 41(3):263-4. Poznani J, Perisi Lj, Urosevi J, Petrucev B, Tosi N, Belej J, KrivokapiDokmanovi L , Jani D, Cvorkov - Drazi M, Bunjevacki G, Pavlovi S. Correlation between Xmn I b - globin gene polymorphism and fetal hemoglobin level in b thalassemia patients in Serbia. Bilt Hematol 2003; 31 (2/3): 53-101. Pavlovic S, Urosevic J, et al. Molecular basis and origin of thalassemia syndromes in Serbia. Acta Haematologica. 2004; 486. Joji NJ, Urosevi J, Boji B, Pavlovi S. Determination of TPMT genotype in the patients with IBD before and during azathioprine therapy. Gastroentherohepatology 2003; 22 (1-2): 5-9. S Pavlovi, N Kovacevi Grujici, T Mitrovi. Identification and characterization of rat b DRE binding factors involved in erythroid-specific induction of transcription. Acta Veterinaria 2003; 53: 67-76. Pavlovic S, Kuzmanovic M, Urosevic J, Poznanic J, Zoranovic T, Djordjevic V, Rasovic N, Bunjevacki G, Cvorkov-Drazic M, Colovic M. Severe central nervous system thrombotic events in hemoglobin Sabine patient. European J Haematol 2003; 71:1-4. Pavlovi S., Urosevi J., Jani D., Kriv "); array_files[153]=new Array(0,4,"http://www.imgge.bg.ac.yu/Srb/Files/molecular_diagnostics.pdf","2009-07-18","1710K"," Microsoft Word - IMGGIwebsitereport.doc ","","","Molecular Diagnostics of Human Diseases The first ICGEB course entitled: Theoretical and Practical Course: Molecular Diagnostics of Human Diseases (16-21 May 2005), ever held in Serbia and Montenegro, was aimed to provide and overview of current knowledge in the field of molecular genetics of human diseases and current technology and applications of molecular techniques for their diagnostics via a mixture of lectures and laboratory work. That was achieved trough combination of lectures given by top level speakers and laboratory sessions. Theoretical part included 10 lectures given by the top speakers in the field, covering following subjects: the genetic and molecular basis of human inherited diseases, European distribution of CFTR gene mutations, mutation databases in modern molecular genetics, basic principles and application of FISH, modifier genes in cystic fibrosis, the role of RNA processing defects, molecular diagnostics of leukemias, molecular pathology of thalasemias, quality assurances in molecular genetic testing and gene therapy. The extensive practical part of the course gave participants the opportunity of hands-on experience in the major techniques of molecular methods for diagnostics of genetic diseases. The laboratory sessions included the following methods: DNA and RNA extraction, PCR and RT PCR, restriction enzyme digestion, agarose and acrylamide electrophoresis, DGGE, SSCP, reverse dot blot, FISH and automatic DNA sequencing. The course venue included library for presentation and laboratory area as well as attractive garden terrace that enabled a relaxed atmosphere and easy mixing of participants. As well as setting up the busy schedule, the organizer also found time to ararnge all together evening with traditional food and drink. This was a late evening, but no mercy was shown and the next day started as usuall at 8:15. The course was attended 18 participants from 9 different countries. The course was considered very interesting and successful by a vast majority of the participants, lectur "); array_files[154]=new Array(0,4,"http://www.imgge.bg.ac.yu/Srb/Files/Festival.pdf","2009-07-18","649K","Festival.pdf ","",""," "); array_files[155]=new Array(0,4,"http://www.imgge.bg.ac.yu/Files/svetskabanka.pdf","2009-07-18","3189K","svetskabanka.pdf ","",""," "); array_files[156]=new Array(0,4,"http://www.imgge.bg.ac.yu/Files/publications08.pdf","2009-07-18","33K"," Microsoft Word - Document1 ","","","Laboratory for Plant Molecular Biology - Publications ­ · Ana M. Brati, Dragana B. Maji, Jelena T. Samardzi and Vesna R. Maksimovi, Functional analysis of the buckwheat metallothionein promoter: tissue specificity pattern and up-regulation under complex stress stimuli, J. Plant Phys. doi:10.1016/j.jplph.2008.12.002 2009 · Bojana Banovic, Nada Surbanovski, Miroslav Konstantinovic, Vesna Maksimovic, Basic RNases of wild almond (Prunus webbii): Cloning and characterization of six new S-RNase and one ``non-S RNase genes, J. Plant Phys. 166, 395-402, 2008. · M. Milisavljevi, G. Timotijevi, S. Radovi, M. Konstantinovi and V. Maksimovi. Two types of aspartic proteinases from buckwheat seed­gene structure and expression analysis. J. Plant Phys. 165, 983-990 (2008) · Nada Surbanovski, Kenneth R. Tobutt, Miroslav Konstantinovic, Vesna Maksimovic, Daniel J. Sargent, Vladimir Stevanovic, Encarnacion Ortega, Radovan I. Boskovic) Self-incompatibility of Prunus tenella and evidence that reproductively isolated species of Prunus have different SFB alleles coupled with an identical S-RNase allele. The Plant Journal 50, 723­734 (2007) · Zivko S. Jovanovi , Jelena D. Milosevi, Svetlana R. Radovi Antioxidative enzymes in the response of buckwheat ( Fagopyrum esculentum Moench) to UV-B radiation , J. Agric. Food Chem., 54, 9472-9478(2006) · Brkljaci J., Maji D., M iljus - uki J., Brati A., Konstantinovi M., Milisavljevi M. and Vesna R. Maksimovi The metallothionein-like gene from buckwheat ­ structural and functional analysis of the promoter region. Int. J. Plant Sci., 166: 889900 (2005) · Milisavljevic MD, Konstantinovic MM, Brkljacic JM, Maksimovic VR. Isolation and computer analysis of the 5 -regulatory region of the seed storage protein gene from buckwheat ( Fagopyrum esculentum Moench) J. Agric. Food Chem. 53: 2076-2080 (2005) · Milisavljevic, M., Timotijevi, G., Radovi, S., Brkljaci, J., Konstantinovi, M., Maksimovi, V. Vicilin-like storage globulin from buckwheat seed. J. Agric. Food Chem. 52, 5258-5262 (2004) · Miljus-Djuki "); array_files[157]=new Array(0,4,"http://www.imgge.bg.ac.yu/Files/publications06.pdf","2009-07-18","54K"," Microsoft Word - Document1 ","","","Laboratory for Molecular Genetics of Industrial Microorganisms - Publications - · ben Omar, N., Valenzuela, A. S., Abriouel, H., López, R. L., Veljovic, K., Caamero, M. M., Kojic, M., Topisirovic, L., Gálvez, A.: Virulence factors, antibiotic resistance, and bacteriocins in enterococci from artisan foods of animal origin. Food Control 20 (2009) 381-385. Strahinic, I., Kojic, M., Tolinacki, M., Fira, D. and Topisirovic, L. Molecular characterization of plasmids pS7a and pS7b from Lactococcus lactis subsp. lactis bv. diacetylactis S50 as a base for the construction of mobilizable cloning vectors. J. Appl. Microbiol. 106 (2009) 78-88. Lozo, J., Begovic, J., Jovcic, B., Golic, N., Topisirovic, L.: Effect of methionine and cysteine deprivation on the growth of different natural isolate of Lactobacillus spp. in chemically defined media. Arch. Biol. Sci. 60 (2008) 509-517. Nikolic, M., Terzic-Vidojevic, A., Jovcic, B., Begovic, J., Golic, N., Topisirovic, L.: Phenotypic and genotypic characterization of the microflora in the autochthonous homemade semi hard goats milk cheese. Int. J. Food Microbiol. 122 (2008) 162-170. Kojic, M., Jovcic, B., Begovic, J., Fira, D., Topisirovic, L.: Large chromosomal inversion correlated with spectinomycin resistance in Lactococcus lactis subsp. lactis bv. diacetylactis S50. Can. J. Microbiol. 54 (2008) 143-149. Busarcevic, M., Kojic M., Dalgalarrondo, M., Chobert, J. M., Haertlé, T., Topisirovic, L.: Purification of bacteriocin LS1 produced by human oral isolate Lactobacillus salivarius BGHO1. Oral. Microbiol. Immunol. 23 (2008) 254-258. Savic, D., Jokovic, N., Topisirovic, L.: Multivariate statistical methods in discrimination of lactobacilli based on their FTIR spectra. Dairy Sci. Tech. 88 (2008) 273-290. Jokovic, N., Nikolic, M., Begovic, J., Jovcic, B., Savic, D. and Topisirovic, L.: A survey of the lactic acid bacteria isolated from Serbian artisanal dairy product kajmak. Int. J Food Microbiol. 127 (2008) 305-311. Jovcic, B., Begovic, J., Lozo, J., Topisirovic, L., Kojic, M.: Post "); array_files[158]=new Array(0,4,"http://www.imgge.bg.ac.yu/Files/publications05.pdf","2009-07-18","39K"," Microsoft Word - Document1 ","","","Laboratory for Molecular Genetics of Actinomycetes - Publications Ili ­ Tomi, T., Mori, I., Conn, G. L., Vasiljevi, B.: Aminoglycoside resistance genes sgm and kgmB protect bacterial but not yeast small ribosomal subunits in vitro despite high conservation of the rRNA site. Res. in Microbiol. 159 (2008) 658 ­ 662. · · Savi, M., Ili ­ Tomi, T., Macmaster, R., Vasiljevi, B., Conn, G. L.: Critical Residues for Cofactor Binding and Catalytic Activity in the Aminoglycoside Resistance Methyltransferase Sgm. J. Bacteriol. 190 (2008) 5855 ­ 5861. · Koji, M., Vojnovi, S., Vukov, N., Vasiljevi, B.: Overexpression of Sgm 5 UTR mRNA reduces gentamicin resistance in both Escherichia coli and Micromonospora melanosporea cells. Arch. Biol.Sci. 59 (2007) 273 ­ 280. · Savi, M., Brati I., Vasiljevi, B.: Streptomyces durmitorensis sp. nov., a producer of an FK506-like immunosuppressant. International Journal of Systematic and Evolutionary Microbiology 57 (2007) 2119-2124. · Savi, M., Vasiljevi, B.: Targeting polyketide synthase gene pool within actinomycetes: new degenerate primers. J. Ind. Microbiol. Biotechnol. 33 (2006) 423430. · Markovi, S., Vojnovi, S., Jovanovi, M., Vasiljevi, B.: Different expression level of two KgmB-His fusion proteins. Journal of the Serbian Chemical Society 70 (2005) 1401-1407. · Ili Tomi, T., Markovi, S., Vasiljevi, B.: Expression and purification of the Sgm protein from E. coli. Journal of the Serbian Chemical Society 70 (2005) 817-822. · Skoko, N., Vujovi, J., Savi, M., Papi, N., Vasiljevi, B., Ljubijanki, G.: Construction of Saccharomyces cerevisiae strain FAV20 useful in detection of immunosuppressants produced by soil actinomycetes. Journal of Microbiological Methods 61 (2005) 137-140. · Vaji, S., Anastasov, N., Vasiljevi, B.: The kgmB gene, encoding ribosomal RNA methylase from Streptomyces tenebrarius, is autogenously regulated. Archives of Microbiology. 182 (2004) 475-481. · Koji, M., Milojevi, N., Vasiljevi, B.: Gentamicin-resistance determinants confer background-dependent hygromycin B resis "); array_files[159]=new Array(0,4,"http://www.imgge.bg.ac.yu/Files/publications04.pdf","2009-07-18","52K"," Microsoft Word - Document1 ","","","Laboratory for Human Molecular Genetics - Publications Petrovi, I., Kovacevi-Grujici, N. and Stevanovi, M. ZBP-89 and Sp3 downregulate while NF-Y up-regulates SOX18 promoter activity in HeLa cells. 2008. Molecular Biology Reports, DOI 10.1007/s11033-008-9272-x. · · Kovacevi Grujici, N., Yokoyama, K. K. and Stevanovi, M. Trans-activation of the human SOX3 promoter by MAZ in NT2/D1 cells. 2008. Archives of Biological Sciences, Belgrade, 60, 379-387. · Nikcevi, G., Savi, T., Kovacevi-Grujici, N. and Stevanovi, M. Up-regulation of the SOX3 gene expression by retinoic acid: characterization of the novel promoterresponse element and the retinoid receptors involved. 2008. Journal of Neurochemistry, 107, 1206-1215. · Krsti, A., Mojsin, M., Kovacevi-Grujici, N. and Stevanovi, M. PCR amplification and sequence analysis of the rat Sox3 gene. 2008. Archives of Biological Sciences, Belgrade, 60, 525-530. · Djordjevi, V., Denci-Fekete, M., Jovanovi, J., Drakuli, D., Stevanovi, M., Jankovi, G. and Goti, M. Pattern of trisomy 1q in hematological malignancies: a single institution experience. 2008. Cancer Genet Cytogenet. 186, 12-18. · Cuturilo, G., Drakulic, D., Stevanovic, M., Jovanovic, I., Djukic, M., Miletic-Grkovic, S. and Atanaskovic-Markovic, M. A rare association of interrupted aortic arch type C and microdeletion 22q11.2. 2008. Eur J Pediatr. 167, 1195-1198. · Krsti, A., Mojsin, M. and Stevanovi, M. Regulation of SOX3 gene expression is driven by multiple NF-Y binding elements. 2007. Archives of Biochemistry and Biophysics, 467, 163-173. · Kovacevi Grujici, N., Mojsin, M., Djurovi, J., Petrovi, I. and Stevanovi, M. Comparison of promoter regions of SOX3, SOX14 and SOX18 orthologs in mammals. 2008. DNA Sequence, 19, 185-194. · Drakuli D., Nikcevi, G., Djordjevi, V. and Stevanovi M. Generation of a whole chromosome painting probe from monochromosomal hybrid cells by the Alu polymerase chain reaction.2007. Archives of Biological Sciences, 59, 89-95. · Nikcevi, G., Kovacevi Grujici, N., Stevanovi, M. Activation of the HSV "); array_files[160]=new Array(0,4,"http://www.imgge.bg.ac.yu/Files/publications03.pdf","2009-07-18","56K"," Microsoft Word - Document1 ","","","Laboratory for Molecular Biology - Publications · M. Stankovic, A. Nikolic, A. Divac, A. Tomovic, N. Petrovic-Stanojevic, M. Andjelic, V. Dopudja-Pantic, M. Surlan, I. Vujicic, D. Ponomarev, M. Mitic-Milikic, J. Kusic,D. Radojkovic. The CFTR M470V gene variant as a potential modifier of COPD severity ­ study of Serbian population. Genet Test. 2008;12(3):357 Stefanovic D, Kusic J, Divac A, Tomic B. Formation of noncanonical DNA structures mediated by human ORC4, a protein component of the origin recognition complex Biochemistry. 2008 Aug 19;47(33):8760-7. Zaimidou S, van Baal S, Smith TD, Mitropoulos K, Ljujic M, Radojkovic D, Cotton RG, Patrinos GP. (2008) A(1)ATVar: a relational database of human SERPINA1 gene variants leading to alpha(1)-antitrypsin deficiency and application of the VariVis software. Hum Mutat. 2008 Nov 19. [Epub ahead of print] Radojkovic M, Ristic S, Colovic M, Cemerikic-Martinovic V, Radojkovic D, Krtolica K. Molecular characteristics and prognostic significance of Bcl-2/IgH gene rearrangement in Serbian follicular lymphoma patients. Neoplasma. 2008; 55(5):4217. Castellani C, Cuppens H, Macek M Jr, Cassiman JJ, Kerem E, Durie P, Tullis E, Assael BM, Bombieri C, Brown A, Casals T, Claustres M, Cutting GR, Dequeker E, Dodge J, Doull I, Farrell P, Ferec C, Girodon E, Johannesson M, Kerem B, Knowles M, Munck A, Pignatti PF, Radojkovic D, Rizzotti P, Schwarz M, Stuhrmann M, Tzetis M, Zielenski J, Elborn JS. Consensus on the use and interpretation of cystic fibrosis mutation analysis in clinical practice. J Cyst Fibros. 2008 May;7(3):179-96. Review. Nisevic I, Dinic J, Nikolic A, Djordjevic V, Lukic S, Ugljesic M, Andjelic-Jelic M, Petrovic-Stanojevic N, Radojkovic D. MTHFR C677T polymorphism in chronic pancreatitis and pancreatic adenocarcinoma. Cell Biochem Funct. 2008;26(6):659-63. Ljujic M, Topic A, Divac A, Nikolic A, Petrovic-Stanojevic N, Surlan M, MiticMilikic M, Radojkovic D. Isoelectric focusing phenotyping and denaturing gradient gel electrophoresis genotyping: a comparison of two met "); array_files[161]=new Array(0,4,"http://www.imgge.bg.ac.yu/Files/publications01.pdf","2009-07-18","44K"," Microsoft Word - Document1 ","","","Laboratory for Molecular Hematology - Publications · Colovic M, Pavlovic S, Kraguljac N, Colovic N, Jankovic G, Sefer D, Tosic N. Acquired amegakaryocytic thrombocytopenia associated with proliferation of gamma/delta TCR T-lymphocytes and a BCR-ABL (p210) fusion transcript. Eur J Haematol. 2004 Nov;73(5):372-375. Poznani J, Perisi Lj, Urosevi J, Petrucev B, ureinovi T, Tosi N, KrivokapiDokmanovi L, Jani D, Cvorkov-Drazi M, Bunjevacki G, Pavlovi S. Biochemical phenotype and origin of the three most common beta-thalassemia mutations in Serbia. Yug Med Bioch 2004. Kollia P, Kalamaras A, Chassanidis C, Samara M, Vamvakopoulos NK, Radmilovi M, Pavlovi S, Papadakis MN, Patrinos GP. Compound heterozygosity for the Cretan type of non-deletional heriditary persistence of fetal hemoglobin and betathalassemia or Hb Sabine confirms the functional role of the A gamma -158 CT mutation in gamma-globin gene transcription. Blood Cells Mol Dis. 2008 Nov-Dec; 41(3):263-4. Poznani J, Perisi Lj, Urosevi J, Petrucev B, Tosi N, Belej J, KrivokapiDokmanovi L , Jani D, Cvorkov - Drazi M, Bunjevacki G, Pavlovi S. Correlation between Xmn I b - globin gene polymorphism and fetal hemoglobin level in b thalassemia patients in Serbia. Bilt Hematol 2003; 31 (2/3): 53-101. Pavlovic S, Urosevic J, et al. Molecular basis and origin of thalassemia syndromes in Serbia. Acta Haematologica. 2004; 486. Joji NJ, Urosevi J, Boji B, Pavlovi S. Determination of TPMT genotype in the patients with IBD before and during azathioprine therapy. Gastroentherohepatology 2003; 22 (1-2): 5-9. S Pavlovi, N Kovacevi Grujici, T Mitrovi. Identification and characterization of rat b DRE binding factors involved in erythroid-specific induction of transcription. Acta Veterinaria 2003; 53: 67-76. Pavlovic S, Kuzmanovic M, Urosevic J, Poznanic J, Zoranovic T, Djordjevic V, Rasovic N, Bunjevacki G, Cvorkov-Drazic M, Colovic M. Severe central nervous system thrombotic events in hemoglobin Sabine patient. European J Haematol 2003; 71:1-4. Pavlovi S., Urosevi J., Jani D., Kriv "); array_files[162]=new Array(0,4,"http://www.imgge.bg.ac.yu/Files/molecular_diagnostics.pdf","2009-07-18","1710K"," Microsoft Word - IMGGIwebsitereport.doc ","","","Molecular Diagnostics of Human Diseases The first ICGEB course entitled: Theoretical and Practical Course: Molecular Diagnostics of Human Diseases (16-21 May 2005), ever held in Serbia and Montenegro, was aimed to provide and overview of current knowledge in the field of molecular genetics of human diseases and current technology and applications of molecular techniques for their diagnostics via a mixture of lectures and laboratory work. That was achieved trough combination of lectures given by top level speakers and laboratory sessions. Theoretical part included 10 lectures given by the top speakers in the field, covering following subjects: the genetic and molecular basis of human inherited diseases, European distribution of CFTR gene mutations, mutation databases in modern molecular genetics, basic principles and application of FISH, modifier genes in cystic fibrosis, the role of RNA processing defects, molecular diagnostics of leukemias, molecular pathology of thalasemias, quality assurances in molecular genetic testing and gene therapy. The extensive practical part of the course gave participants the opportunity of hands-on experience in the major techniques of molecular methods for diagnostics of genetic diseases. The laboratory sessions included the following methods: DNA and RNA extraction, PCR and RT PCR, restriction enzyme digestion, agarose and acrylamide electrophoresis, DGGE, SSCP, reverse dot blot, FISH and automatic DNA sequencing. The course venue included library for presentation and laboratory area as well as attractive garden terrace that enabled a relaxed atmosphere and easy mixing of participants. As well as setting up the busy schedule, the organizer also found time to ararnge all together evening with traditional food and drink. This was a late evening, but no mercy was shown and the next day started as usuall at 8:15. The course was attended 18 participants from 9 different countries. The course was considered very interesting and successful by a vast majority of the participants, lectur "); array_files[163]=new Array(0,4,"http://www.imgge.bg.ac.yu/Files/glisin.pdf","2009-07-18","167K"," Microsoft Word - VG-CV konacna verzija.doc ","","","CURRICULUM VITAE NAME: VLADIMIR R. GLISIN BORN: June 24, 1930, Novi Sad, Yugoslavia ADDRESS: Institute of Molecular Genetics and Genetic Engineering, Emeritus Director, Vojvode Stepe 444a, P.O.Box 446, 11001 Beograd, Yugoslavia EDUCATION: 1956 - Graduate of Chemistry, Faculty of Sciences and Mathematics, University of Belgrade. 1963 - Ph.D. in Biology, Faculty of Sciences and Mathematics University of Belgrade. PROFESSIONAL and SCIENTIFIC CAREER: 1960 - Research Fellow at the Institute for Biophysics of Macromolecules, Polish Academy of Sciences, Warsaw. Head of the laboratory, prof. David Shugar. 1961-1963 - Research Fellow in the Laboratory of prof. Paul Doty, Chemistry Department, Harvard University, Cambridge, Mass. USA. 1963-1967 - Continued appointment as a Research Fellow in Prof. Paul Dotys laboratory. 1966 - Head, Laboratory of Molecular Embryology, Marine Station, Kotor, Yugoslavia. 1967-1971 - Senior Scientist, Institute for Biological Research, Belgrade, Yugoslavia. 1968 ­ 1971 - Head, Laboratory of Molecular Biology, Institute for Biological Research, Belgrade, Yugoslavia. 1969-1970 - Visiting Associate Professor, Dept. of Chemistry, Harvard University. 1970 - Senior Foreign Scientist Award, National Science Foundation, USA 1971-1985 - Professor of Molecular Embryology, Center for Multidisciplinary Studies and Faculty of Sciences, University of Belgrade, Yugoslavia 1973-1974 - Visiting Professor, Dept. of Biochemistry, University of New Hampshire. 1975-1977 - Head, Laboratory of Molecular Biology and Embryology, Institute for Nuclear Sciences B. Kidric Belgrade, Yugoslavia. 1983-1992 Director, Affiliated Center in Belgrade, of the UNIDO Center for Genetic Engineering and Biotechnology 1985-1995 - Director, Institute of Molecular Genetics and Genetic Engineering (IMGGE), Belgrade, Yugoslavia. 1986-1992 - Special UNIDO Scientific Adviser for Asia, Africa and Europe for the New Biotechnology. 1988-1990 - Professor of Biochemistry, Faculty of Medicine, University of Kuwait. 1994 - present ­ Member, Sci "); array_files[164]=new Array(0,4,"http://www.imgge.bg.ac.yu/Files/Festival.pdf","2009-07-18","649K","Festival.pdf ","",""," "); file_count=165;